| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,705,460 | T→A | 15.0% | intergenic (‑152/+25) | kdpF ← / ← PP_4162 | potassium‑transporting ATPase subunit F/membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,705,460 | 0 | T | A | 15.0% | 75.4 / 5.8 | 33 | intergenic (‑152/+25) | kdpF/PP_4162 | potassium‑transporting ATPase subunit F/membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (13/14); new base A (2/3); total (15/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 | |||||||||||
GTGGATCTCCCCGTGTTACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACA > NC_002947/4705311‑4705609 | gtgGATCTCCCCGTGTTACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGGGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCtt > 2:638876/1‑150 (MQ=255) tgGATCTCCCCGTGTTACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGGTGGGGTCTGCCTGGAACGTCCTCTTCGCGGGCTTg > 2:472607/1‑150 (MQ=255) gATCTCCCCGTGTTACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCGTCTTCGCGGGCTTGcc > 1:686097/1‑150 (MQ=255) ccggggtTAGGGCTGGGGCGGTTTTGGGGAAATTGTCGGGGGGAGGGGCGCAAAGGGCCGAAGGGGGGGGGGGGGTGTTGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCAAGCCCGCGaa < 2:627650/145‑1 (MQ=255) cccGTGTTACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCCTTTCGCGGGCCAGCCcgctca > 2:204517/1‑147 (MQ=255) cccGTGTTACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCCAGATCGGGGGATGGGGGTTGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGaa > 1:531212/1‑150 (MQ=255) ccGTGTTACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGaaa > 1:587264/1‑150 (MQ=255) gggtTACGGCTTGGGCCGCTTTGGGGAAAGAGTCCGGGGGAAAGGAGTAAAGGGGGGAGATGGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCAGCCTGTAACGGCCCTTTCGCGGGCAAGCCCGCGaagag < 1:561596/148‑4 (MQ=25) ggtTAGGTCTTGGGTGGTTTTGGTGAAATTGTCGGGGGGAGGGGGGTAAGGGGGCGAGGGGGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCCTTTCGCGGGCAAGCCCGCGaagagg < 1:204517/149‑5 (MQ=19) ttACGGTTGGGGCCGCTGGGGTGAAATTGCCCGAGGGGGTGGCGTAAGGGGGCGAGACCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGcc < 2:740674/150‑1 (MQ=255) ttACGGCTTGGGCCGCTTTGGGGAAATTGTCCGGGGGAGAGGCGTAAAGGGGCGGGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGcc < 2:587264/150‑1 (MQ=255) cgccgtGGGCCGCTTTGGGGAAATTGTCCGAAGGAGAGGCGTAACGGGGCGAGGCGGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGtt < 1:29321/145‑1 (MQ=255) gggCCGCTTTGGGGAAAGTGGCCGAGGGAGAGGCGTAAAGGGGCGAGATGGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGc < 2:382972/150‑1 (MQ=255) gAAATTGTCCGAGGGAGAGGCGTAAAGGGGCTAGATCGGGGGGGGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTgga > 1:19705/1‑149 (MQ=255) tCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGgctgct > 1:486221/1‑150 (MQ=255) ggggggAGGGGGGAAAAGGGGCGAGATGGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCtgctgc < 2:686097/147‑1 (MQ=255) gggAGGGGGGTAAGGGGGGGAGGTGGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGcgcc < 2:827832/150‑1 (MQ=255) ggCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTa < 2:531212/150‑1 (MQ=255) ggCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGTGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCtccagt > 1:56716/1‑150 (MQ=255) gagaTGGGGGGGGGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGACGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCtccagtcca < 1:236039/150‑1 (MQ=255) agaTCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCtccagtccag > 1:520951/1‑150 (MQ=255) gaTCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCcagc > 2:529336/1‑150 (MQ=255) gggggtgggTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTa < 2:56716/150‑1 (MQ=255) tgtATAAAGAAAGCGTACACATCAGCGTTGTGGTCTGCCTGTAACGCCCTCTTCGCGGGCCGGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTAcc < 1:162622/149‑1 (MQ=255) tataAAGAATGCGTAAAAATCAGCGTTGTGGTCTGAATGGAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGc < 1:472607/150‑1 (MQ=255) taAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCa < 1:638876/150‑1 (MQ=255) tgtgGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGc < 2:486221/150‑1 (MQ=255) tgGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCa > 2:363050/1‑150 (MQ=255) aaCGGCCTCTTCGCGGGCTTGCACGCGACAGAGCCATTACATTCTTATTGTTGTTGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAg < 2:520951/150‑1 (MQ=255) gCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCTGCTGCTTCGCCGCGTTACGGCTCCAGTCCAGCAGCACGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgcaa > 2:15913/1‑150 (MQ=255) gCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgcaa > 1:310659/1‑150 (MQ=255) cctttCGCGGGCAAGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgcaagc > 1:51406/4‑150 (MQ=255) ctTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAg < 2:466601/94‑1 (MQ=255) ctTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAg > 1:466601/1‑94 (MQ=255) ttGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGACGTTGCTTCGCCGCGTTACGGGTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAac > 2:29920/1‑150 (MQ=255) tGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAaca < 2:310659/150‑1 (MQ=255) | GTGGATCTCCCCGTGTTACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACA > NC_002947/4705311‑4705609 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |