| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,711,190 | A→G | 10.5% | V133A (GTT→GCT) | gpsA ← | glycerol‑3‑phosphate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,711,190 | 0 | A | G | 10.5% | 98.0 / 3.3 | 38 | V133A (GTT→GCT) | gpsA | glycerol‑3‑phosphate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (19/15); new base G (2/2); total (21/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.51e-01 | |||||||||||
TGGCGTAGACGCGGAAGGTGCGCCCGTGCAGTACGGCCTGTACCTGCTGGCACAGGTCTTCGTGCTCACTGGCCACCACTGTGGCAGTCAGCGCATGTTCGGCGATTTCGCGCGCCAGGTTGGGCCCCGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTGCGCTTCGATGCCCTTGGTCAGGCTGACCAGGCCCTTGCCGCGCAGCAGCTCGGCATGCGGTGCCAGCACGCTGCGCAAGGCACTC > NC_002947/4711056‑4711336 | tGGCGTAGACGCGGAAGGTGCGCCCGTGCAGTACGGCCTGTACCTGCTGGCACAGGTCTTCGTGCTCACTGGCCACCACTGTGGCAGTCAGCGCATGTTCGACCATTTTCGGCGTTCCATTGGCCCCCGACAAAACGCCTATTCGCGCCt > 2:93948/1‑150 (MQ=255) gCGTAGACGCGGAAGGTGCGCCCGTGCAGTACGGCCTGTACCTGCTGGCACAGGTCTTCGTGCTCACTGGCCACCACTGTGGCAGTCAGCGCATGTTCGGCGATTTCGCGCGCCAGGTTGGGCCCCGACAAAACGACGATGCGCGCCTCg > 1:386286/1‑150 (MQ=255) gTAGACGCGGAAGGTGCGCCCGTGCAGTACGGCCTGTACCTGCTGGCACAGGTCTTCGTGCTCACTGGCCACCACTGTGGCAGTCAGCGCATGTTCGGCGATTTCGCGCGCCAGGTTGGGCCCCGACAAAACGCCGATGCGCGCCTCggg > 1:831220/1‑150 (MQ=255) gACGCGGAAGGTGCGCCCGTGCAGTACGGCCTGTACCTGCTGGCACAGGTCTTCGTGCTCACTGGCCACCACTGTGGCAGTCAGCGCATGTTCGGCGATTTCGCGCGCCAGGTTGGGCCCCGACAAAACGCCGATGCGCGCCTCGGGGGc < 2:290959/150‑1 (MQ=255) aCGCGGAAGGTGCGCCCGTGCAGTACGGCCTGTACCTGCTGGCACAGGTCTTCGTGCTCACTGGCCACCACTGTGGCAGTCAGCGCATGTTCGGCGATTTCGCGCGCCAGGTTGGGCCCCGACAAAACGCCGATGCGCGCCTCGGGGGCg > 2:519163/1‑150 (MQ=255) ggTGCGCCCGTGCAGTACGGCCTGTACCTGCTGGCACAGGTCTTCGTGCTCACTGGCCACCACTGTGGCAGTCAGCGCATGTTCGGCGATTTCGCGCGCCAGGTTGGGCCCCGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTc < 2:784667/150‑1 (MQ=255) tGCGCCCGTGCAGTACGGCCTGTACCTGCTGGCACAGGTCTTCGTGCTCACTGGCCACCACTGTGGCAGTCAGCGCATGTTCGGCGATTTCGCGCGCCAGGTTGGGCCCCGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGa < 1:519163/150‑1 (MQ=255) tatccTGTGCCAGCAGGTACAGGTCTTCGTGCTCACTGGCCACCACTGTGGCAGTCAGCGCATGTTCGGCGATTTCGCGCGCCAGGTTGGGCCCCGACACAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCtcatca > 1:803265/4‑150 (MQ=255) ctgctgGCACAGGTCTTCGTGCTCACTGGCCACCACTGTGGCAGTCAGCGCATGTTCGGCGATTTCGCGCGCCAGGTTGTGCCCCGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCt > 1:668977/1‑150 (MQ=255) tgctgGCACAGGTCTTCGTGCTCACTGGCCACCACTGTGGCAGTCAGCGCATGTTCGGCGATTTCGCGCGCCAGGTTGTGCCCCGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCtt < 2:668977/150‑1 (MQ=255) tgGCACAGGTCTTCGTGCTCACTGGCCACCACTGTGGCAGTCAGCGCATGTTCGGCGATTTCGCGCGCCAGGTTGGGCCCCGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTgc < 1:93948/150‑1 (MQ=255) gCACAGGTCTTCGTGCTCACTGGCCACCACTGTGGCAGTCAGCGCATGTTCGGCGATTTCGCGCGCCAGGTTGGGCCCCGACAAAGCGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTgcgc > 1:248535/1‑150 (MQ=255) gCACAGGTCTTCGTGCTCACTGGCCACCACTGTGGCAGTCAGCGCATGTTCGGCGATTTCGCGCGCCAGGTTGGGCCCCGACAAAGCGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTgcgc < 2:248535/150‑1 (MQ=255) gCACAGGTCTTCGTGCTCACTGGCCACCACTGTGGCAGTCAGCGCATGTTCGGCGATTTCGCGCGCCAGGTTGGGCCCCGACAAAGCGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTgcgc < 2:248229/150‑1 (MQ=255) gCACAGGTCTTCGTGCTCACTGGCCACCACTGTGGCAGTCAGCGCATGTTCGGCGATTTCGCGCGCCAGGTTGGGCCCCGACAAAGCGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTgcgc > 1:248229/1‑150 (MQ=255) cacaGGTCTTCGTGCTCACTGGCCACCACTGTGGCAGTCAGCGCATGTTCGGCGATTTCGGGCGCCAGGTTGGGCCCCGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTGCGCt > 2:247874/1‑150 (MQ=255) caGGTCTTCGTGCTCACTGGCCACCACTGTGGCAGTCAGCGCATGTTCGGCGATTTCGCGCGCCAGGTTGGGCCCCGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTGCGCTTc < 1:63995/150‑1 (MQ=255) ttCGTGCTCACTGGCCACCACTGTGGCAGTCAGCGCATGTTCGGCGATTTCGCGCGCCAGGTTGGGCCCCGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTGCGCTTCGATGcc > 1:571875/1‑150 (MQ=255) aCTGGCCACCACTGTGGCAGTCAGCGCATGTTCGGCGATTTCGCGCGCCAGGTTGGGCCCCGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCt < 1:680082/119‑1 (MQ=255) aCTGGCCACCACTGTGGCAGTCAGCGCATGTTCGGCGATTTCGCGCGCCAGGTTGGGCCCCGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCt > 2:680082/1‑119 (MQ=255) gCCACCACTGTGGCAGTCAGCGCATGTTCGGCGATTTCGCGCGCCAGGTTGGGCCCCGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTGCGCTTCGATGCCCTTGGTCAgg > 2:558215/1‑147 (MQ=255) gCCACCACTGTGGCAGTCAGCGCATGTTCGGCGATTTCGCGCGCCAGGTTGGGCCCCGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTGCGCTTCGATGCCCTTGGTCAgg < 1:558215/147‑1 (MQ=255) tgtgGCAGTCAGCGCATGTTCGGCGATTTCACGCGCCCGGTTGAGTCCGGTGACGAGCAGGATGCGCGCTTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTGCGCTTCGATGCCCTTGGTCAGGCTGACCAGGcc > 1:16649/1‑150 (MQ=255) tgGCAGTCAGCGCATGTTCGGCGATTTCGCGCGCCAGGTTGGGCCCCGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTGCGCTTCGATGCCCTTGGTCAGGCTGACCAGGCCCt > 1:776875/1‑150 (MQ=255) gcgcATGTTCGGCGATTTCGCGCGCCAGGTTGGGCCCCGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTGCGCTTCGATGCCCTTGGTCAGGCTGACCAGGCCCTTGCCGCgca > 1:46548/1‑150 (MQ=255) gcgcATGTTCGGCGATTTCGCGCGCCAGGTTGGGCCCCGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTGCGCTTCGATGCCCTTGGTCAGCCTCACCAGCCCCTTGCCgcgcc > 2:832523/1‑149 (MQ=255) gcgcATGTTCGGCGATTTCGCGCGCCAGGTTGGGCCCCGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCAAGGATCTGGCTCATCAGCTTGAAGCTTTGCGCTTCGATGCCCTTGGTCAGGCTGACCAGGCCCTTGCCGCgca < 1:48603/150‑1 (MQ=255) gATTTCGCGCGCCAGGTTGGGCCCCGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTGCGCTTCGATGCCCTTGGTCAGGCTGACCAGGCCCTTg < 1:559307/130‑1 (MQ=255) gATTTCGCGCGCCAGGTTGGGCCCCGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTGCGCTTCGATGCCCTTGGTCAGGCTGACCAGGCCCTTg > 2:559307/1‑130 (MQ=255) gcgcgcCAGGTTGGGCCCCGACAAAATGGCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTGCGCTTCGATGCCCTTGGTCAGGCTGACCAGGCCCTTGCCGCGCAGCAGCTCGGCATGCGGTGc > 1:239766/1‑150 (MQ=255) gcCAGGTTGGGCCCCGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTGCGCTTCGATGccc > 2:807418/1‑96 (MQ=255) gcCAGGTTGGGCCCCGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCCTCGAGGATCTGGCTCATCAGCTTGAAGCTTTGCGCTTCGATGccc < 1:807418/96‑1 (MQ=255) ccccGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTGCGCTTCGATGCCCTTGGTCAGGCTGACCAGGCCCTTGCCGCGCAGCAGCTCGGCATGCGGTGCCAGCACGCTGCGCaa < 2:386286/150‑1 (MQ=255) ccccGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTGCGCTTCGATGCCCTTGGTCAGGCTGACCAGGCCCTTGCCGCGCAGCAGCTCGGCATGCGGTGCCAGCACGCTGCGCaa < 1:247874/150‑1 (MQ=255) ccGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCCCGAAATCATCGCGCCAGCTTGAAGCTTTGCGCTTCGATGCCCTTGGTCAGGCTGACCAGGCCCTTGCCGCGCAGCAGCTCGGCATGCGGTGCCAGCACGCTGCGCAAgg < 2:239766/150‑1 (MQ=255) cGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTGCGCTTCGATGCCCTTGGTCAgg > 1:835948/1‑91 (MQ=255) cGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTGCGCTTCGATGCCCTTGGTCAgg < 2:835948/91‑1 (MQ=255) gACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTGCGCTTCGATGCCCTTGGTCAGGCTGACCAGGCCCTTGCCGCGCAGCAGCTCGGCATGCGGTGCCAGCACGCTGCGCAAGGCa > 1:267993/1‑150 (MQ=255) aaaaCGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTGCGCTTCGATGCCCTTGGTCAGGCTGACCAGGCCCTTGCCGCGCAGCAGCTCGGCATGCGGTGCCAGCACGCTGCGCAAGGCACTc < 2:633567/150‑1 (MQ=255) | TGGCGTAGACGCGGAAGGTGCGCCCGTGCAGTACGGCCTGTACCTGCTGGCACAGGTCTTCGTGCTCACTGGCCACCACTGTGGCAGTCAGCGCATGTTCGGCGATTTCGCGCGCCAGGTTGGGCCCCGACAAAACGCCGATGCGCGCCTCGGGGGCGATCTCTTCGAGGATCTGGCTCATCAGCTTGAAGCTTTGCGCTTCGATGCCCTTGGTCAGGCTGACCAGGCCCTTGCCGCGCAGCAGCTCGGCATGCGGTGCCAGCACGCTGCGCAAGGCACTC > NC_002947/4711056‑4711336 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |