| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 26,844 | A→T | 16.7% | intergenic (+40/+145) | PP_0021 → / ← PP_0022 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 26,844 | 0 | A | T | 16.7% | 71.0 / 8.2 | 30 | intergenic (+40/+145) | PP_0021/PP_0022 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (14/11); new base T (3/2); total (17/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01 | |||||||||||
CTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGGTGTC > NC_002947/26706‑26988 | cTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGTAAACCACTGTGGttacag < 1:637970/150‑5 (MQ=255) gacGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTATAAAATAAAAAAAAGt < 2:744742/148‑1 (MQ=255) ccGGTTGATCATCCTTTTCGGTGTCTTGGGAGATGTAAGAGGGCCCGGAAGGACCTGGGCATTTTCAATGCTCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTg < 1:656633/150‑1 (MQ=16) ccGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACGGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTg < 1:428410/150‑1 (MQ=255) tGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑ATCCACAGTGAAAAAAAAAAAAAAAAAGTGGTTTa < 2:602801/150‑1 (MQ=255) ttATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGtt < 1:166233/139‑1 (MQ=255) ttATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGtt > 2:166233/1‑139 (MQ=255) ttATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGc > 2:439179/1‑150 (MQ=255) aTAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGccc > 1:667341/1‑150 (MQ=255) gCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACaa < 1:519927/116‑1 (MQ=255) gCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACaa > 2:519927/1‑116 (MQ=255) gggCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGctcct > 1:265705/1‑150 (MQ=255) ctgGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGTAAACCACTGTGG‑TTACAGTg > 1:201902/1‑74 (MQ=17) ctgGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGTAAACCACTGTGG‑TTACAGTg < 2:201902/74‑1 (MQ=17) tCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTAGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGc > 2:251412/1‑150 (MQ=255) aCCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGTTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGGCAGCGCGCCCCTCATTGATTCGTTCAGTCGGGCGGCGAACACCTGTGCTGCTCGGGGc > 1:622365/1‑150 (MQ=39) ccACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGcc > 1:2915/1‑150 (MQ=255) cACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCt > 1:686930/1‑150 (MQ=255) cTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGtt < 1:303684/112‑1 (MQ=255) cTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGtt > 2:303684/1‑112 (MQ=255) ttttttCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGcc < 1:514638/150‑1 (MQ=255) tCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTg < 1:134907/143‑1 (MQ=255) tCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTg > 2:134907/1‑143 (MQ=255) aCTGTAAACCACTGTGG‑TTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCAcgccgc > 2:220581/1‑150 (MQ=255) ctgtaaaCCACTGTGG‑TTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCcgcg > 1:577698/6‑150 (MQ=255) tGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCggg < 2:255229/133‑1 (MQ=255) tGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCggg > 1:255229/1‑133 (MQ=255) gT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGgt > 1:683415/1‑150 (MQ=255) t‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGgtg > 2:522121/1‑150 (MQ=255) aCCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGGTGTc < 2:683415/150‑1 (MQ=255) | CTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGGTGTC > NC_002947/26706‑26988 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |