Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,760,260	T→G	10.0%	F93V (TTC→GTC)	pvdM →	dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,760,260	0	T	G	10.0%	95.4 / 2.7	37	F93V (TTC→GTC)	pvdM	dipeptidase
Reads supporting (aligned to +/- strand):  ref base T (21/12);  new base G (2/2);  total (23/14)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCGACAAGGACGGCTACCCCATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGG‑‑CACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGCGACATGCCCAGGAAGTGCGCTAC  >  NC_002947/4760116‑4760409                                                                                                                                                   |                                                                                                                                                      ttCGACAAGGACGGCTACCCCATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTAGG‑‑CACCGAGGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGAc                                                                                                                                                  >  1:696787/1‑150 (MQ=255)   cGACAAGGACGGCTACCCCATAGAGGTCGTGCGCCAGGACAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGG‑‑CACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCt                                                                                                                                                >  2:494368/1‑150 (MQ=255)      cAAGGACGGCTACCCCATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCTTCTC‑CCGAAACGGT‑CACGTAGGTTTCCCGAGCCCCACGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGc                                                                                                                                             >  1:316532/1‑150 (MQ=255)       aaGGACGGCTACCCCATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGG‑‑CACCGAAGGCCGCCAAGCTGACAAGGATGGCCCGGGCCAGGTCGAACTGGcc                                                                                                                                            >  1:485830/1‑150 (MQ=255)          gACGGCTACCCCATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGG‑‑CACCGAAGGCCGCCAAGCTGACAAGGATGGCCCGGGCCAGGTCGAACTGGcccgg                                                                                                                                         <  2:485830/150‑4 (MQ=255)           aCGGCTACCCCATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCCCTCTTCCGCTGGACGTCGG‑‑CACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGGTCGAACTGGcccggg                                                                                                                                        <  1:104982/150‑5 (MQ=255)                tACCCCATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGG‑‑CACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCcgcg                                                                                                                                   >  1:688607/1‑150 (MQ=255)                             tCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGACGCTGGACTTCGG‑‑CACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGt                                                                                                                      <  2:243904/150‑1 (MQ=255)                                   gcCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGG‑‑CACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGgcg                                                                                                                >  1:336822/1‑150 (MQ=255)                                         ccAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGG‑‑CACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGccc                                                                                                          <  1:23000/150‑1 (MQ=255)                                            aTGATCTGCAAGAGCGGATAATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGG‑‑CACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGa                                                                                                       <  2:825479/150‑1 (MQ=255)                                              gATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGG‑‑CACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGAcc                                                                                                     >  1:732682/1‑150 (MQ=255)                                                       gagCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGG‑‑CACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGGTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCGTGACCGTCTTCGCt                                                                                            >  2:245303/1‑150 (MQ=255)                                                                 tttCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGG‑‑CACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGt                                                                                  <  2:316532/150‑1 (MQ=255)                                                                  ttcgttcgACAGCCATCTCACCCTGCCGCTGGACTTCGG‑‑CACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGTCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGTCCTGACCGTCTTCGCTTGGCCCGAGTc                                                                                 >  2:416741/1‑150 (MQ=255)                                                                       tcgACAGCCATCTCACCCTGCCGCTGGACTTCGG‑‑CACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCcgc                                                                                                                                    <  1:473981/94‑1 (MQ=255)                                                                       tcgACAGCCATCTCACCCTGCCGCTGGACTTCGG‑‑CACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCcgc                                                                                                                                    >  2:473981/1‑94 (MQ=255)                                                                         gACAGCCATCTCACCCTGCCGCTGGACTTCGG‑‑CACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGAcc                                                                          >  2:79948/1‑150 (MQ=255)                                                                           cAGCCATCTCACCCTGCCGCTGGACTTCGG‑‑CACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCgg                                                                        >  1:212564/1‑150 (MQ=255)                                                                              ccATCTCACCCTGCCGCTGGACTTCGG‑‑CACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGa                                                                     <  2:336822/150‑1 (MQ=255)                                                                              ccATCTCACCCTGCCGCTGGACTTCGG‑‑CACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGa                                                                     <  2:515590/150‑1 (MQ=255)                                                                                     ccccTGCCGCTGGACTTCGG‑‑CACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGc                                                              <  2:696787/149‑1 (MQ=255)                                                                                       ccTGCCGCTGGACTTCGG‑‑CACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACg                                                                <  1:175532/146‑1 (MQ=255)                                                                                       ccTGCCGCTGGACTTCGG‑‑CACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGCCCTGGACCGGCGACAACg                                                                >  2:175532/1‑146 (MQ=255)                                                                                                        g‑‑CACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACg                                           >  2:717505/1‑150 (MQ=255)                                                                                                           cACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGACCTGACCTTCTTCGCTGGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACgg                                          >  2:523799/1‑150 (MQ=255)                                                                                                             ccGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGtt                                        >  2:560463/1‑150 (MQ=255)                                                                                                               gAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTtc                                      >  1:506090/1‑150 (MQ=255)                                                                                                                  ggCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTtcgtc                                   >  1:276694/1‑150 (MQ=255)                                                                                                                      gcgAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGTCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGAtt                               >  1:242646/1‑149 (MQ=255)                                                                                                                            cTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGc                         <  2:506090/150‑1 (MQ=255)                                                                                                                               aCAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGCGAc                      >  1:281495/1‑150 (MQ=255)                                                                                                                                     aTGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGCGACATGccc                >  1:413964/1‑150 (MQ=255)                                                                                                                                             ggCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGCGACATGCCCAGGAAGTg        <  1:500433/150‑1 (MQ=255)                                                                                                                                               ccAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGCGACATGCCCAGGAAGtctc      >  2:171472/1‑147 (MQ=255)                                                                                                                                               ccAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGCGACATGCCCAGGAAGTgcg      >  1:70912/1‑150 (MQ=255)                                                                                                                                                cAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGCGACATGCCCAGGAAGTgcgc     <  2:70912/150‑1 (MQ=255)                                                                                                                                                   ttCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGCGACATGCCCAGGAAGTGCGCTAc  >  1:128435/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                      TTCGACAAGGACGGCTACCCCATAGAGGTCGTGCGCCAGGCCAATGATCTGCAAGAGCGGATCATTTCGTTCGACAGCCATCTCACCCTGCCGCTGGACTTCGG‑‑CACCGAAGGCCGCGAAGCTGACAAGGATGGCCCGGGCCAGTTCGACCTGGCCAAAGCCGCGCGCGGGCGACTGTCCGGCGCTGCCCTGACCGTCTTCGCTTGGCCCGAGTCCTGGACCGGCGACAACGGCCCGCACCACCCCACCGACGGTTTCGTCGATGCCGGGCGACATGCCCAGGAAGTGCGCTAC  >  NC_002947/4760116‑4760409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑