Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,765,973 C→G 10.0% A194G (GCG→GGG)  fpvA → TonB‑dependent outer membrane ferripyoverdine receptor FpvA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,765,9730CG10.0% 106.2 / 4.7 40A194G (GCG→GGG) fpvATonB‑dependent outer membrane ferripyoverdine receptor FpvA
Reads supporting (aligned to +/- strand):  ref base C (20/16);  new base G (2/2);  total (22/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

ATGCAGACCGCGACCAAACTGCCACTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCGAGATCGTGCGGGGCG  >  NC_002947/4765828‑4766116
                                                                                                                                                 |                                                                                                                                               
aTGCAGACCGCGACCAAACTGCCACTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCgcgcgc                                                                                                                                             >  1:468980/1‑150 (MQ=255)
  gCAGACCGCGACCAAACTGCCACTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGccc                                                                                                                                           <  2:13643/150‑1 (MQ=255)
  gCAGACCGCGACCAAACTGCCACTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGccc                                                                                                                                           >  1:13643/1‑150 (MQ=255)
    aGACCGCGACCAAACTGCCACTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCgg                                                                                                                                         >  2:125242/1‑150 (MQ=255)
        cgcgACCAAACTGCCACTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCaa                                                                                                                                     >  2:458999/1‑150 (MQ=255)
                        cTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTcc                                                                                                                     >  2:696000/1‑150 (MQ=255)
                             ccTGCGCGAAACCCCGCAGGCCGTGACGGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGGACTTTTCCCGCgg                                                                                                                >  1:416642/1‑150 (MQ=255)
                              cTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGc                                                                                                               >  1:819877/1‑150 (MQ=255)
                               tGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCt                                                                                                              >  1:227115/1‑150 (MQ=255)
                               tGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGCCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCt                                                                                                              >  2:118696/1‑150 (MQ=255)
                                  gcgAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTAc                                                                                                                           >  2:575831/1‑134 (MQ=255)
                                  gcgAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTAc                                                                                                                           <  1:575831/134‑1 (MQ=255)
                                        ccccGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCg                                                                                                                                          <  1:49293/113‑1 (MQ=255)
                                        ccccGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCg                                                                                                                                          >  2:49293/1‑113 (MQ=255)
                                             gagcgcGTCGCCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCaacaac                                                                                                <  2:819877/145‑1 (MQ=255)
                                                  cGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGAt                                                                                           <  1:696000/150‑1 (MQ=255)
                                                   gTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCACGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATg                                                                                          <  1:447372/150‑1 (MQ=255)
                                                        cGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGa                                                                                     >  1:249707/1‑150 (MQ=255)
                                                         gTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGAt                                                                                    <  1:125242/150‑1 (MQ=255)
                                                                             ggACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACaa                                                                <  1:102735/150‑1 (MQ=255)
                                                                                 gacCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGcc                                                            <  2:227115/150‑1 (MQ=255)
                                                                                 gacCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGACGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCcca                                                                          <  1:286146/136‑1 (MQ=255)
                                                                                 gacCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGACGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCcca                                                                          >  2:286146/1‑136 (MQ=255)
                                                                                    cAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAAcc                                                                                               <  2:201775/112‑1 (MQ=255)
                                                                                    cAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAAcc                                                                                               >  1:201775/1‑112 (MQ=255)
                                                                                    cAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGAc                                                         >  1:88111/1‑150 (MQ=255)
                                                                                    cAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGAc                                                         >  1:139809/1‑150 (MQ=255)
                                                                                                    acgacgaCGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCg                                         >  2:586717/2‑150 (MQ=255)
                                                                                                          acgTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATgg                                   <  2:88111/150‑1 (MQ=255)
                                                                                                            gTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGgcg                                 >  1:745890/1‑150 (MQ=255)
                                                                                                              ggTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGcc                               <  1:88961/150‑1 (MQ=255)
                                                                                                               gTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGccc                              >  2:477364/1‑150 (MQ=255)
                                                                                                                     gCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGAc                        <  1:802085/150‑1 (MQ=255)
                                                                                                                       cACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACcg                      >  2:514555/1‑150 (MQ=255)
                                                                                                                             tGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCga                <  1:477364/150‑1 (MQ=255)
                                                                                                                                        tgttggCCGGGCGCGCGGCCGACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGAc                        <  2:709976/127‑1 (MQ=255)
                                                                                                                                        tGTTGGCCGGGCGCGCGGCCGACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGAc                        >  1:709976/1‑131 (MQ=255)
                                                                                                                                        tGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCGAGATCGTGCggg     <  2:249707/150‑1 (MQ=255)
                                                                                                                                          ttggCCGGGCGCGCGGCCGACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCGAGATCGTGCGGGgc   <  2:171109/148‑1 (MQ=255)
                                                                                                                                          ttggCCGGGCGCGCGGCCGACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCGAGATCGTGCGGGgc   >  1:171109/3‑150 (MQ=255)
                                                                                                                                           cGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCGAGATCGTGCGGGgcg  >  2:111879/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
ATGCAGACCGCGACCAAACTGCCACTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCGAGATCGTGCGGGGCG  >  NC_002947/4765828‑4766116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑