Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,765,978 C→G 10.2% P196A (CCG→GCG)  fpvA → TonB‑dependent outer membrane ferripyoverdine receptor FpvA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,765,9780CG10.2% 116.3 / 4.5 40P196A (CCG→GCG) fpvATonB‑dependent outer membrane ferripyoverdine receptor FpvA
Reads supporting (aligned to +/- strand):  ref base C (20/15);  new base G (2/2);  total (22/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

GCAGACCGCGACCAAACTGCCACTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCGAGATCGTGCGGGGCG  >  NC_002947/4765830‑4766116
                                                                                                                                                    |                                                                                                                                          
gCAGACCGCGACCAAACTGCCACTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGccc                                                                                                                                           <  2:13643/150‑1 (MQ=255)
gCAGACCGCGACCAAACTGCCACTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGccc                                                                                                                                           >  1:13643/1‑150 (MQ=255)
  aGACCGCGACCAAACTGCCACTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCgg                                                                                                                                         >  2:125242/1‑150 (MQ=255)
      cgcgACCAAACTGCCACTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCaa                                                                                                                                     >  2:458999/1‑150 (MQ=255)
                      cTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTcc                                                                                                                     >  2:696000/1‑150 (MQ=255)
                           ccTGCGCGAAACCCCGCAGGCCGTGACGGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGGACTTTTCCCGCgg                                                                                                                >  1:416642/1‑150 (MQ=255)
                            cTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGc                                                                                                               >  1:819877/1‑150 (MQ=255)
                             tGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCt                                                                                                              >  1:227115/1‑150 (MQ=255)
                             tGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGCCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCt                                                                                                              >  2:118696/1‑150 (MQ=255)
                                gcgAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTAc                                                                                                                           <  1:575831/134‑1 (MQ=255)
                                gcgAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTAc                                                                                                                           >  2:575831/1‑134 (MQ=255)
                                      ccccGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCg                                                                                                                                          <  1:49293/113‑1 (MQ=255)
                                      ccccGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCg                                                                                                                                          >  2:49293/1‑113 (MQ=255)
                                           gagcgcGTCGCCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCaacaac                                                                                                <  2:819877/145‑1 (MQ=255)
                                                cGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGAt                                                                                           <  1:696000/150‑1 (MQ=255)
                                                 gTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCACGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATg                                                                                          <  1:447372/150‑1 (MQ=255)
                                                      cGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGa                                                                                     >  1:249707/1‑150 (MQ=255)
                                                       gTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGAt                                                                                    <  1:125242/150‑1 (MQ=255)
                                                                           ggACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACaa                                                                <  1:102735/150‑1 (MQ=255)
                                                                               gacCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGcc                                                            <  2:227115/150‑1 (MQ=255)
                                                                               gacCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGACGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCcca                                                                          <  1:286146/136‑1 (MQ=255)
                                                                               gacCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGACGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCcca                                                                          >  2:286146/1‑136 (MQ=255)
                                                                                  cAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAAcc                                                                                               >  1:201775/1‑112 (MQ=255)
                                                                                  cAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAAcc                                                                                               <  2:201775/112‑1 (MQ=255)
                                                                                  cAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGAc                                                         >  1:88111/1‑150 (MQ=255)
                                                                                  cAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGAc                                                         >  1:139809/1‑150 (MQ=255)
                                                                                                  acgacgaCGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCg                                         >  2:586717/2‑150 (MQ=255)
                                                                                                        acgTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATgg                                   <  2:88111/150‑1 (MQ=255)
                                                                                                          gTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGgcg                                 >  1:745890/1‑150 (MQ=255)
                                                                                                            ggTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGcc                               <  1:88961/150‑1 (MQ=255)
                                                                                                             gTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGccc                              >  2:477364/1‑150 (MQ=255)
                                                                                                                   gCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGAc                        <  1:802085/150‑1 (MQ=255)
                                                                                                                     cACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACcg                      >  2:514555/1‑150 (MQ=255)
                                                                                                                           tGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCga                <  1:477364/150‑1 (MQ=255)
                                                                                                                                      tgttggCCGGGCGCGCGGCCGACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGAc                        <  2:709976/127‑1 (MQ=255)
                                                                                                                                      tGTTGGCCGGGCGCGCGGCCGACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGAc                        >  1:709976/1‑131 (MQ=255)
                                                                                                                                      tGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCGAGATCGTGCggg     <  2:249707/150‑1 (MQ=255)
                                                                                                                                        ttggCCGGGCGCGCGGCCGACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCGAGATCGTGCGGGgc   <  2:171109/148‑1 (MQ=255)
                                                                                                                                        ttggCCGGGCGCGCGGCCGACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCGAGATCGTGCGGGgc   >  1:171109/3‑150 (MQ=255)
                                                                                                                                         cGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCGAGATCGTGCGGGgcg  >  2:111879/1‑150 (MQ=255)
                                                                                                                                                  gcCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGAt                                                                                    >  2:582299/1‑59 (MQ=255)
                                                                                                                                                  gcCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGAt                                                                                    <  1:582299/59‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                          
GCAGACCGCGACCAAACTGCCACTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCGAGATCGTGCGGGGCG  >  NC_002947/4765830‑4766116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑