| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,765,984 | A→G | 10.3% | N198D (AAC→GAC) | fpvA → | TonB‑dependent outer membrane ferripyoverdine receptor FpvA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,765,984 | 0 | A | G | 10.3% | 96.8 / 3.2 | 39 | N198D (AAC→GAC) | fpvA | TonB‑dependent outer membrane ferripyoverdine receptor FpvA |
| Reads supporting (aligned to +/- strand): ref base A (19/16); new base G (2/2); total (21/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.40e-01 | |||||||||||
CGCGACCAAACTGCCACTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCGAGATCGTGCGGGGCGCCACCGGCATGATGCA > NC_002947/4765836‑4766132 | cgcgACCAAACTGCCACTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCaa > 2:458999/1‑150 (MQ=255) cTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTcc > 2:696000/1‑150 (MQ=255) ccTGCGCGAAACCCCGCAGGCCGTGACGGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGGACTTTTCCCGCgg > 1:416642/1‑150 (MQ=255) cTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGc > 1:819877/1‑150 (MQ=255) tGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCt > 1:227115/1‑150 (MQ=255) tGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGCCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCt > 2:118696/1‑150 (MQ=255) gcgAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTAc < 1:575831/134‑1 (MQ=255) gcgAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTAc > 2:575831/1‑134 (MQ=255) gagcgcGTCGCCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCaacaac < 2:819877/145‑1 (MQ=255) cGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGAt < 1:696000/150‑1 (MQ=255) gTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCACGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATg < 1:447372/150‑1 (MQ=255) cGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGa > 1:249707/1‑150 (MQ=255) gTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGAt < 1:125242/150‑1 (MQ=255) ggACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACaa < 1:102735/150‑1 (MQ=255) gacCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGcc < 2:227115/150‑1 (MQ=255) gacCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGACGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCcca < 1:286146/136‑1 (MQ=255) gacCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGACGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCcca > 2:286146/1‑136 (MQ=255) cAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAAcc > 1:201775/1‑112 (MQ=255) cAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAAcc < 2:201775/112‑1 (MQ=255) cAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGAc > 1:139809/1‑150 (MQ=255) cAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGAc > 1:88111/1‑150 (MQ=255) acgacgaCGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCg > 2:586717/2‑150 (MQ=255) acgTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATgg < 2:88111/150‑1 (MQ=255) gTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGgcg > 1:745890/1‑150 (MQ=255) ggTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGcc < 1:88961/150‑1 (MQ=255) gTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGccc > 2:477364/1‑150 (MQ=255) gCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGAc < 1:802085/150‑1 (MQ=255) cACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACcg > 2:514555/1‑150 (MQ=255) tGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCga < 1:477364/150‑1 (MQ=255) tgttggCCGGGCGCGCGGCCGACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGAc < 2:709976/127‑1 (MQ=255) tGTTGGCCGGGCGCGCGGCCGACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGAc > 1:709976/1‑131 (MQ=255) tGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCGAGATCGTGCggg < 2:249707/150‑1 (MQ=255) ttggCCGGGCGCGCGGCCGACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCGAGATCGTGCGGGgc > 1:171109/3‑150 (MQ=255) ttggCCGGGCGCGCGGCCGACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCGAGATCGTGCGGGgc < 2:171109/148‑1 (MQ=255) cGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCGAGATCGTGCGGGgcg > 2:111879/1‑150 (MQ=255) gcCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGAt > 2:582299/1‑59 (MQ=255) gcCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGAt < 1:582299/59‑1 (MQ=255) cGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCGAGATCGTGCGGGGCGCCACCGGCatga < 2:468980/150‑1 (MQ=255) cAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCGAGATCGTGCGGGGCGCCACCGGCATGATGCa > 1:767693/1‑150 (MQ=255) cAACAGCGAGTACTTTTCCCGCAGCTTTCCCATCAACAACCTGATGTTCGATTGACTGCCCACCACCTACAACGCCGACTGGGACGCAGCGGGCGACAGAGCGCCCTACGACCGCGGCGAGATCGTGCGGGGCGCCACCGGCATGATGCa > 2:204078/1‑150 (MQ=255) | CGCGACCAAACTGCCACTGACCCTGCGCGAAACCCCGCAGGCCGTCACCGTGATCACCCGCCAGCGCATGGACGACCAGTCGATGCGTAGCCTCGACGACGTGGTCCAGGCCACTCCTGGTCTGCGCATGTCGGCCGCGCGCCCGGCCAACAGCGAGTACTTTTCCCGCGGCTTTCCCATCAACAACCTGATGTTCGATGGCCTGCCCACCACCTACAACGCCGACTGGGTCGCCGCGGCCGACATGGCGCCCTACGACCGCGTCGAGATCGTGCGGGGCGCCACCGGCATGATGCA > NC_002947/4765836‑4766132 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |