Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,768,309 T→G 8.7% E183A (GAG→GCG)  PP_4218 ← lipase/esterase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,768,3090TG 8.7% 103.1 / 2.4 43E183A (GAG→GCG) PP_4218lipase/esterase family protein
Reads supporting (aligned to +/- strand):  ref base T (22/16);  new base G (2/2);  total (24/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

GCACCGTGGCGTATTGCTGGTAGAACCACTCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAGCGCCAGGCATCCAGGGCATCGTCACTGGCT  >  NC_002947/4768166‑4768458
                                                                                                                                               |                                                                                                                                                     
gCACCGTGGCGTATTGCTGGTAGAACCACTCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCt                                                                                                                                                 >  1:457413/1‑150 (MQ=255)
   ccGTGGCGTATTGCTGGTAGAACCACTCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCGCGAGCTgcc                                                                                                                                              <  1:185484/150‑2 (MQ=255)
     gcggCGTATTGCTGGTAGAACCACTCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGCCACCGGGTCGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCa                                                                                                                                            >  2:813824/3‑150 (MQ=255)
       ggCGTATTGCTGGTAGAACCACTCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGc                                                                                                                                          <  2:272801/150‑1 (MQ=255)
        gCGTATTTCTGGTAGAACCACTCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTACCGGCAGCTCGCGCTGCGCAGcc                                                                                                                                         >  2:826577/1‑150 (MQ=255)
                cTGGTAGAACCACTCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCCACTGGtt                                                                                                                                 >  2:128517/1‑150 (MQ=255)
                cTGGTAGAACCACTCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGtt                                                                                                                                 <  2:801675/150‑1 (MQ=255)
                cTGGTAGAACCACTCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTACCGGCAGCTCGCGCTGCGCAGCCAACTGGtt                                                                                                                                 <  1:826577/150‑1 (MQ=255)
                      gAACCACTCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAg                                                                                                                           >  1:371699/1‑150 (MQ=255)
                      gAACCACT‑CAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGa                                                                                                                          >  1:387904/1‑150 (MQ=255)
                         ccACTCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAAt                                                                                                                        >  2:325486/1‑150 (MQ=255)
                            cTCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGt                                                                                                                     <  2:822598/150‑1 (MQ=255)
                             tCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTa                                                                                                                    <  1:288316/150‑1 (MQ=255)
                                      tGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTa                                                                                                           >  1:599062/1‑150 (MQ=255)
                                           ttCAAGCAGGTAACCGCTACCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGAGCATTACCTGCAGGCGCGGTGCGGGCACCGCGCGCTGCGCAGCCAACTGGTTGGCCAGAACGGTAGCCAGGCTaccacc                                                                                                      >  2:685802/1‑150 (MQ=255)
                                                gCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCCGGCTACCACCGacac                                                                                                 >  2:410507/1‑150 (MQ=255)
                                                    gTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTc                                                                                             >  1:206796/1‑150 (MQ=255)
                                                               cATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCAGAGGTCACCGGGGAGCTCATTAACTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTCGTTGGCCAGGGTGGTAGGCGGGCTACCACCGACCCGGTCGCCCcccaccc                                                                                  >  2:82843/1‑149 (MQ=255)
                                                                aTAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGc                                                                                 <  2:830555/150‑1 (MQ=255)
                                                                aTAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGGAGCCAGGCTACCACCGACACTGTCGCCCACCACCGc                                                                                 >  1:293150/1‑150 (MQ=255)
                                                                            cGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTgcgc                                                                     >  2:550875/1‑150 (MQ=255)
                                                                              aaGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCaa                                                                   >  2:749023/1‑149 (MQ=255)
                                                                                  ccgccgGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGAt                                                               >  1:78600/1‑150 (MQ=255)
                                                                                          aaCTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAgcg                                                       >  2:119226/1‑150 (MQ=255)
                                                                                              ggCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTc                                                   <  1:119226/150‑1 (MQ=255)
                                                                                               gccctggTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCg                                                  >  1:29868/6‑150 (MQ=255)
                                                                                               gCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCg                                                  <  2:371699/150‑1 (MQ=255)
                                                                                                 aTCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGc                                                >  1:578540/1‑150 (MQ=255)
                                                                                                   cGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCt                                              <  1:325486/150‑1 (MQ=255)
                                                                                                     gTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCTGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGt                                                                                                                     <  1:653323/77‑1 (MQ=255)
                                                                                                     gTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCTGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGt                                                                                                                     >  2:653323/1‑77 (MQ=255)
                                                                                                         ccGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCTAGCGCCTCGGCCTGCTCGa                                        <  1:629792/150‑1 (MQ=255)
                                                                                                                gATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGaccaacca                                 <  2:682363/150‑1 (MQ=255)
                                                                                                                 aTCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAg                                >  1:461678/1‑150 (MQ=255)
                                                                                                                      caCCTGCAGGCGCGGTGCCGGCAGCCCGCGCTGCGCAGCCAACTGGTTCGCCAGAATGGTAGCCAGGCTACCACCGACACCGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAGCGCCa                           <  2:599062/149‑1 (MQ=255)
                                                                                                                              ggCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGacc                                      <  2:490973/131‑1 (MQ=255)
                                                                                                                              ggCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGacc                                      >  1:490973/1‑131 (MQ=255)
                                                                                                                               gcgcGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAGCGCCAGGCATCCAg                  >  1:461985/1‑150 (MQ=255)
                                                                                                                                cgcgGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAGCGCCAGGCATCCAgg                 <  2:461678/150‑1 (MQ=255)
                                                                                                                                       tgcgCAGCGCGAGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAGCGCCAGGCATCCAGGGCATCGt          >  1:78299/4‑150 (MQ=255)
                                                                                                                                         ggCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAGCGCCAGGCATCCAGGGCATCGTCa        <  1:550875/150‑1 (MQ=255)
                                                                                                                                          gCAGCGCGAGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAGCGCCAGGCATCCAGGGCATCGTCAc       <  2:78299/150‑1 (MQ=255)
                                                                                                                                             gCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAGCGCCAGGCATCCAGGGCATCGTCACTgg    <  2:457413/150‑1 (MQ=255)
                                                                                                                                               tCGCACTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCTATGCCCAACGCCTCGGCCTGCTCGACCAACCAGCGCCAGGCATCCAGGGCATCGTCACTGGCt  >  2:77724/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
GCACCGTGGCGTATTGCTGGTAGAACCACTCCAGGGTCTGCGCTTCAAGCAGGTAACCGCTGCCATAGCGCTGCACCGAAGGCCGCCGGCAACTGGCATCGGTCACCGGGTAGATCATTACCTGCAGGCGCGGTGCCGGCAGCTCGCGCTGCGCAGCCAACTGGTTGGCCAGAATGGTAGCCAGGCTACCACCGACACTGTCGCCCACCACCGCCAGTCGCTGCGCATCGATGCCCAGCGCCTCGGCCTGCTCGACCAACCAGCGCCAGGCATCCAGGGCATCGTCACTGGCT  >  NC_002947/4768166‑4768458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑