Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,820,857 T→C 7.7% M3460V (ATG→GTG)  pvdL ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,820,8570TC 7.7% 145.2 / 2.2 52M3460V (ATG→GTG) pvdLnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base T (19/29);  new base C (2/2);  total (21/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

CACGTCGCGGTCGCCGCTGTAGCGCGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGATGTAATCGCGGTAACGCGGT  >  NC_002947/4820708‑4821005
                                                                                                                                                     |                                                                                                                                                    
cACGTCGCGGTCGCCGCTGTAGCGCGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCAt                                                                                                                                                      <  1:686327/150‑1 (MQ=255)
   gTCGCGGTCGCCGCTGTAGCGCGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGcc                                                                                                                                                   <  2:681251/150‑1 (MQ=255)
    tCGCGGTCGCCGCTGTAGCGCGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCg                                                                                                                                                  <  2:670667/150‑1 (MQ=255)
     cgcgGTCGCCGCTGTAGCGAGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGc                                                                                                                                                 <  1:471677/150‑1 (MQ=255)
      gcgGTCGCCGCTGTAGCGCGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCt                                                                                                                                                >  2:218555/1‑150 (MQ=255)
              cgcTGTAGCGCGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTAACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCC‑ATGACCACGCCGGTAGCGGcatg                                                                                                                                       >  1:450347/1‑147 (MQ=255)
               gcTGTAGCGCGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCACGCCGGTAGCGGCATg                                                                                                                                       <  1:141251/150‑1 (MQ=255)
               gcTGTAGCGCGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCACGCCGGTAGCGGCATg                                                                                                                                       <  2:14699/150‑1 (MQ=255)
               gcTGTAGCGCGACAGCACCAGGGCCCAGGCAGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTg                                                                                                                                       <  1:732362/150‑1 (MQ=255)
                     gcgcGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATg                                                                                                                                 <  2:433276/150‑1 (MQ=255)
                            aGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGTTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAgc                                                                                                                          >  1:788056/1‑150 (MQ=255)
                                 cAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCCTGGATTTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGAGGGCATGGCGCAgcgggcg                                                                                                                     >  1:53713/1‑150 (MQ=255)
                                 cAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAgcgggcg                                                                                                                     <  2:837151/150‑1 (MQ=255)
                                       ccAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCt                                                                                                               >  1:317330/1‑150 (MQ=255)
                                          ggCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCgg                                                                                                            <  2:332156/150‑1 (MQ=255)
                                             cgccTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGAt                                                                                                         <  1:714446/150‑1 (MQ=255)
                                                      gAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGc                                                                                                >  2:255842/1‑150 (MQ=255)
                                                       aaaGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCa                                                                                               >  1:479302/1‑150 (MQ=255)
                                                        aaGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCAt                                                                                                                                  <  1:421009/114‑1 (MQ=255)
                                                        aaGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCAt                                                                                                                                  >  2:421009/1‑114 (MQ=255)
                                                            gtTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTc                                                                                          <  2:317330/150‑1 (MQ=255)
                                                               gACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGaa                                                                                       <  2:479302/150‑1 (MQ=255)
                                                                   gTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCg                                                                                   <  1:174402/150‑1 (MQ=255)
                                                                   gTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCg                                                                                   <  2:788056/150‑1 (MQ=255)
                                                                   gTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCg                                                                                   <  2:272158/150‑1 (MQ=255)
                                                                   gTGAGCTGATGGGCCCGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGACGCCg                                                                                   <  2:595299/150‑1 (MQ=255)
                                                                     gAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGc                                                                                 >  1:798645/1‑150 (MQ=255)
                                                                       gCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCa                                                                               <  1:255842/150‑1 (MQ=255)
                                                                         tGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAgg                                                                             <  1:218555/150‑1 (MQ=255)
                                                                                cctgggccAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGTCGGGTATAACAATCGCCGACGACCCTGCCGCCACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCCCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCt                                                                      <  2:320154/150‑1 (MQ=255)
                                                                                      ccAGTTCGCGCAGACGCACGCTATTATTCATATCAAGGCGGGTATAACAAACGCCGACGATCGTGCCGCTAACGGCGTGGTCAAGGGGCAGAGGGCGGTCGCTCGTGATGGCTGTGGTGCGTTCGGAGCCGCCCAGGTTGGCCTGccacc                                                                >  2:117420/1‑150 (MQ=255)
                                                                                       cAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGc                                                                                                                                                 <  2:387373/68‑1 (MQ=255)
                                                                                       cAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGc                                                                                                                                                 >  1:387373/1‑68 (MQ=255)
                                                                                       cAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGccacca                                                               >  2:455540/1‑150 (MQ=255)
                                                                                         gTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAgg                                                             <  2:677166/150‑1 (MQ=255)
                                                                                          ttCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGc                                                            <  1:836674/150‑1 (MQ=255)
                                                                                                       acgcCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTcc                                               >  2:377085/1‑150 (MQ=255)
                                                                                                            atcatTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGcc                                          >  2:500283/1‑150 (MQ=255)
                                                                                                                      tcAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGc                                >  2:589412/1‑150 (MQ=255)
                                                                                                                        aaGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCt                                                                      >  2:32029/1‑110 (MQ=255)
                                                                                                                        aaGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCt                                                                      <  1:32029/110‑1 (MQ=255)
                                                                                                                                tataACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGa                      >  1:366863/1‑150 (MQ=255)
                                                                                                                                 ataACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGAt                     <  2:205706/150‑1 (MQ=255)
                                                                                                                                 ataACAACCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGAt                     <  1:117420/150‑1 (MQ=255)
                                                                                                                                     cAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCa                               >  1:703494/1‑136 (MQ=255)
                                                                                                                                     cAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCa                               <  2:703494/136‑1 (MQ=255)
                                                                                                                                      aaTCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGt                                                        >  2:124435/1‑110 (MQ=255)
                                                                                                                                      aaTCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGt                                                        <  1:124435/110‑1 (MQ=255)
                                                                                                                                       aTCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGATGTAATc               <  1:455540/150‑1 (MQ=255)
                                                                                                                                          gCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGATGTAATcgcg            <  2:798645/150‑1 (MQ=255)
                                                                                                                                             catgacCACGCCGGTAGCGGCATGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGATGTAATCGCGGTa         >  1:102803/4‑150 (MQ=255)
                                                                                                                                                   cATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGATGTAATCGCGGTAACGCgg   <  2:700552/150‑1 (MQ=255)
                                                                                                                                                    aTGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGATGTAATCGCGGTAACGCGGt  >  2:789761/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CACGTCGCGGTCGCCGCTGTAGCGCGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGATGTAATCGCGGTAACGCGGT  >  NC_002947/4820708‑4821005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑