Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,820,862 C→G 8.5% S3458T (AGC→ACC)  pvdL ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,820,8620CG 8.5% 139.1 / 5.1 47S3458T (AGC→ACC) pvdLnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base C (18/25);  new base G (2/2);  total (20/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01

CGCGGTCGCCGCTGTAGCGCGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGATGTAATCGCGGTAACGCGGT  >  NC_002947/4820713‑4821005
                                                                                                                                                     |                                                                                                                                               
cgcgGTCGCCGCTGTAGCGAGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGc                                                                                                                                                 <  1:471677/150‑1 (MQ=255)
 gcgGTCGCCGCTGTAGCGCGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCt                                                                                                                                                >  2:218555/1‑150 (MQ=255)
         cgcTGTAGCGCGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTAACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCC‑ATGACCACGCCGGTAGCGGcatg                                                                                                                                       >  1:450347/1‑147 (MQ=255)
          gcTGTAGCGCGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCACGCCGGTAGCGGCATg                                                                                                                                       <  1:141251/150‑1 (MQ=255)
          gcTGTAGCGCGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCACGCCGGTAGCGGCATg                                                                                                                                       <  2:14699/150‑1 (MQ=255)
          gcTGTAGCGCGACAGCACCAGGGCCCAGGCAGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTg                                                                                                                                       <  1:732362/150‑1 (MQ=255)
                gcgcGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATg                                                                                                                                 <  2:433276/150‑1 (MQ=255)
                       aGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGTTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAgc                                                                                                                          >  1:788056/1‑150 (MQ=255)
                            cAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCCTGGATTTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGAGGGCATGGCGCAgcgggcg                                                                                                                     >  1:53713/1‑150 (MQ=255)
                            cAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAgcgggcg                                                                                                                     <  2:837151/150‑1 (MQ=255)
                                  ccAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCt                                                                                                               >  1:317330/1‑150 (MQ=255)
                                     ggCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCgg                                                                                                            <  2:332156/150‑1 (MQ=255)
                                        cgccTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGAt                                                                                                         <  1:714446/150‑1 (MQ=255)
                                                 gAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGc                                                                                                >  2:255842/1‑150 (MQ=255)
                                                  aaaGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCa                                                                                               >  1:479302/1‑150 (MQ=255)
                                                   aaGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCAt                                                                                                                                  <  1:421009/114‑1 (MQ=255)
                                                   aaGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCAt                                                                                                                                  >  2:421009/1‑114 (MQ=255)
                                                       gtTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTc                                                                                          <  2:317330/150‑1 (MQ=255)
                                                          gACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGaa                                                                                       <  2:479302/150‑1 (MQ=255)
                                                              gTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCg                                                                                   <  2:788056/150‑1 (MQ=255)
                                                              gTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCg                                                                                   <  1:174402/150‑1 (MQ=255)
                                                              gTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCg                                                                                   <  2:272158/150‑1 (MQ=255)
                                                              gTGAGCTGATGGGCCCGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGACGCCg                                                                                   <  2:595299/150‑1 (MQ=255)
                                                                gAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGc                                                                                 >  1:798645/1‑150 (MQ=255)
                                                                  gCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCa                                                                               <  1:255842/150‑1 (MQ=255)
                                                                    tGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAgg                                                                             <  1:218555/150‑1 (MQ=255)
                                                                           cctgggccAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGTCGGGTATAACAATCGCCGACGACCCTGCCGCCACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCCCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCt                                                                      <  2:320154/150‑1 (MQ=255)
                                                                                 ccAGTTCGCGCAGACGCACGCTATTATTCATATCAAGGCGGGTATAACAAACGCCGACGATCGTGCCGCTAACGGCGTGGTCAAGGGGCAGAGGGCGGTCGCTCGTGATGGCTGTGGTGCGTTCGGAGCCGCCCAGGTTGGCCTGccacc                                                                >  2:117420/1‑150 (MQ=255)
                                                                                  cAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGc                                                                                                                                                 >  1:387373/1‑68 (MQ=255)
                                                                                  cAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGc                                                                                                                                                 <  2:387373/68‑1 (MQ=255)
                                                                                  cAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGccacca                                                               >  2:455540/1‑150 (MQ=255)
                                                                                    gTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAgg                                                             <  2:677166/150‑1 (MQ=255)
                                                                                     ttCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGc                                                            <  1:836674/150‑1 (MQ=255)
                                                                                                  acgcCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTcc                                               >  2:377085/1‑150 (MQ=255)
                                                                                                       atcatTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGcc                                          >  2:500283/1‑150 (MQ=255)
                                                                                                                 tcAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGc                                >  2:589412/1‑150 (MQ=255)
                                                                                                                   aaGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCt                                                                      >  2:32029/1‑110 (MQ=255)
                                                                                                                   aaGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCt                                                                      <  1:32029/110‑1 (MQ=255)
                                                                                                                           tataACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGa                      >  1:366863/1‑150 (MQ=255)
                                                                                                                            ataACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGAt                     <  2:205706/150‑1 (MQ=255)
                                                                                                                            ataACAACCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGAt                     <  1:117420/150‑1 (MQ=255)
                                                                                                                                cAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCa                               >  1:703494/1‑136 (MQ=255)
                                                                                                                                cAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCa                               <  2:703494/136‑1 (MQ=255)
                                                                                                                                 aaTCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGt                                                        <  1:124435/110‑1 (MQ=255)
                                                                                                                                 aaTCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGt                                                        >  2:124435/1‑110 (MQ=255)
                                                                                                                                  aTCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGATGTAATc               <  1:455540/150‑1 (MQ=255)
                                                                                                                                     gCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGATGTAATcgcg            <  2:798645/150‑1 (MQ=255)
                                                                                                                                        catgacCACGCCGGTAGCGGCATGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGATGTAATCGCGGTa         >  1:102803/4‑150 (MQ=255)
                                                                                                                                              cATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGATGTAATCGCGGTAACGCgg   <  2:700552/150‑1 (MQ=255)
                                                                                                                                               aTGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGATGTAATCGCGGTAACGCGGt  >  2:789761/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
CGCGGTCGCCGCTGTAGCGCGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGATGTAATCGCGGTAACGCGGT  >  NC_002947/4820713‑4821005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑