Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,820,865 C→G 8.5% G3457A (GGT→GCT)  pvdL ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,820,8650CG 8.5% 142.1 / 3.3 47G3457A (GGT→GCT) pvdLnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base C (16/26);  new base G (2/2);  total (19/28)
Fisher's exact test for biased strand distribution p-value = 6.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

CGCTGTAGCGCGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGATGTAATCGCGGTAACGCGGTGGCACCGG  >  NC_002947/4820722‑4821013
                                                                                                                                               |                                                                                                                                                    
cgcTGTAGCGCGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTAACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCC‑ATGACCACGCCGGTAGCGGcatg                                                                                                                                               >  1:450347/1‑147 (MQ=255)
 gcTGTAGCGCGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCACGCCGGTAGCGGCATg                                                                                                                                               <  2:14699/150‑1 (MQ=255)
 gcTGTAGCGCGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCACGCCGGTAGCGGCATg                                                                                                                                               <  1:141251/150‑1 (MQ=255)
 gcTGTAGCGCGACAGCACCAGGGCCCAGGCAGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTg                                                                                                                                               <  1:732362/150‑1 (MQ=255)
       gcgcGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATg                                                                                                                                         <  2:433276/150‑1 (MQ=255)
              aGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGTTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAgc                                                                                                                                  >  1:788056/1‑150 (MQ=255)
                   cAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCCTGGATTTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGAGGGCATGGCGCAgcgggcg                                                                                                                             >  1:53713/1‑150 (MQ=255)
                   cAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAgcgggcg                                                                                                                             <  2:837151/150‑1 (MQ=255)
                         ccAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCt                                                                                                                       >  1:317330/1‑150 (MQ=255)
                            ggCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCgg                                                                                                                    <  2:332156/150‑1 (MQ=255)
                               cgccTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGAt                                                                                                                 <  1:714446/150‑1 (MQ=255)
                                        gAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGc                                                                                                        >  2:255842/1‑150 (MQ=255)
                                         aaaGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCa                                                                                                       >  1:479302/1‑150 (MQ=255)
                                          aaGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCAt                                                                                                                                          <  1:421009/114‑1 (MQ=255)
                                          aaGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCAt                                                                                                                                          >  2:421009/1‑114 (MQ=255)
                                              gtTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTc                                                                                                  <  2:317330/150‑1 (MQ=255)
                                                 gACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGaa                                                                                               <  2:479302/150‑1 (MQ=255)
                                                     gTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCg                                                                                           <  1:174402/150‑1 (MQ=255)
                                                     gTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCg                                                                                           <  2:272158/150‑1 (MQ=255)
                                                     gTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCg                                                                                           <  2:788056/150‑1 (MQ=255)
                                                     gTGAGCTGATGGGCCCGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGACGCCg                                                                                           <  2:595299/150‑1 (MQ=255)
                                                       gAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGc                                                                                         >  1:798645/1‑150 (MQ=255)
                                                         gCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCa                                                                                       <  1:255842/150‑1 (MQ=255)
                                                           tGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAgg                                                                                     <  1:218555/150‑1 (MQ=255)
                                                                  cctgggccAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGTCGGGTATAACAATCGCCGACGACCCTGCCGCCACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCCCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCt                                                                              <  2:320154/150‑1 (MQ=255)
                                                                        ccAGTTCGCGCAGACGCACGCTATTATTCATATCAAGGCGGGTATAACAAACGCCGACGATCGTGCCGCTAACGGCGTGGTCAAGGGGCAGAGGGCGGTCGCTCGTGATGGCTGTGGTGCGTTCGGAGCCGCCCAGGTTGGCCTGccacc                                                                        >  2:117420/1‑150 (MQ=255)
                                                                         cAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGccacca                                                                       >  2:455540/1‑150 (MQ=255)
                                                                           gTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAgg                                                                     <  2:677166/150‑1 (MQ=255)
                                                                            ttCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGc                                                                    <  1:836674/150‑1 (MQ=255)
                                                                                         acgcCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTcc                                                       >  2:377085/1‑150 (MQ=255)
                                                                                              atcatTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGcc                                                  >  2:500283/1‑150 (MQ=255)
                                                                                                        tcAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGc                                        >  2:589412/1‑150 (MQ=255)
                                                                                                          aaGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCt                                                                              <  1:32029/110‑1 (MQ=255)
                                                                                                          aaGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCt                                                                              >  2:32029/1‑110 (MQ=255)
                                                                                                                  tataACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGa                              >  1:366863/1‑150 (MQ=255)
                                                                                                                   ataACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGAt                             <  2:205706/150‑1 (MQ=255)
                                                                                                                   ataACAACCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGAt                             <  1:117420/150‑1 (MQ=255)
                                                                                                                       cAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCa                                       >  1:703494/1‑136 (MQ=255)
                                                                                                                       cAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCa                                       <  2:703494/136‑1 (MQ=255)
                                                                                                                        aaTCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGt                                                                >  2:124435/1‑110 (MQ=255)
                                                                                                                        aaTCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGt                                                                <  1:124435/110‑1 (MQ=255)
                                                                                                                         aTCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGATGTAATc                       <  1:455540/150‑1 (MQ=255)
                                                                                                                            gCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGATGTAATcgcg                    <  2:798645/150‑1 (MQ=255)
                                                                                                                               catgacCACGCCGGTAGCGGCATGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGATGTAATCGCGGTa                 >  1:102803/4‑150 (MQ=255)
                                                                                                                                     cATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGATGTAATCGCGGTAACGCgg           <  2:700552/150‑1 (MQ=255)
                                                                                                                                      aTGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGATGTAATCGCGGTAACGCGGt          >  2:789761/1‑150 (MQ=255)
                                                                                                                                              aCCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCTGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGATGTAATCGCGGTAACGCGGTGGCACCgg  <  1:36260/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CGCTGTAGCGCGACAGCACCAGGGCCCAGGCCGCCTGGGCGAAAGTGTTGACGGTGAGCTGATGGGCCTGGGCCAGTTCGCGCAGACGCACGCCATCATTCATCTCAAGGCGGGTATAACAATCGCCGACGACCATGCCGCTACCGGCGTGGTCATGGCGCAGCGGGCGGTCGCTCGGGATGGCTGTGGCACGTTCGAAGCCGGCCAGGTTGGCCTGCCACCAGGCACGTGCGTCGTCCAGGCCCTGGCGTTGCAGCCAACCGATGTAATCGCGGTAACGCGGTGGCACCGG  >  NC_002947/4820722‑4821013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑