Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,824,317 G→C 12.1% S2306R (AGC→AGG)  pvdL ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,824,3170GC12.1% 117.1 / 6.3 42S2306R (AGC→AGG) pvdLnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base G (18/18);  new base C (3/2);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

ACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGCGGGTACTCGGGG  >  NC_002947/4824174‑4824461
                                                                                                                                               |                                                                                                                                                
aCCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTggcg                                                                                                                                            >  2:123233/1‑150 (MQ=255)
  cGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTggcggc                                                                                                                                          >  1:642625/1‑150 (MQ=255)
       ggCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGt                                                                                                                                     <  2:67713/150‑1 (MQ=255)
          aGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGCTCATTTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCtt                                                                                                                                  <  2:286210/150‑1 (MQ=255)
              cATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAg                                                                                                                              <  2:642625/150‑1 (MQ=255)
                      gttCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGc                                                                                                                      >  1:404004/3‑150 (MQ=255)
                         cGCCATGGCTCACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGCCCAGCGTTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTgg                                                                                                                   <  2:332738/150‑1 (MQ=255)
                                      caccacGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAgg                                                                                                      >  1:177324/1‑150 (MQ=255)
                                      caccacGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAgg                                                                                                      <  1:123233/150‑1 (MQ=255)
                                         cacGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAg                                                                                                   >  1:288661/1‑150 (MQ=255)
                                              ccTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTTCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGCCTggcggcc                                                                                                                                         <  1:105618/107‑2 (MQ=255)
                                              ccTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGc                                                                                              >  1:842410/1‑150 (MQ=255)
                                              ccTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGCCTggcggcc                                                                                                                                         >  2:105618/1‑106 (MQ=255)
                                                 tGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCa                                                                                           <  2:351389/150‑1 (MQ=255)
                                                   ggTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAgg                                                                                         <  1:125969/150‑1 (MQ=255)
                                                    gTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGCCTggcggcctaca                                                                                                                                     >  1:57581/1‑100 (MQ=255)
                                                    gTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGCCTggcggcctaca                                                                                                                                     <  2:57581/105‑6 (MQ=255)
                                                     tttGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGAgt                                                                                       >  1:570936/1‑150 (MQ=255)
                                                          cGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCa                                                                                                        >  1:598166/1‑128 (MQ=255)
                                                          cGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCa                                                                                                        <  2:598166/128‑1 (MQ=255)
                                                                    tGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACg                                                                        >  1:808802/1‑150 (MQ=255)
                                                                    tGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACg                                                                        >  1:301875/1‑150 (MQ=255)
                                                                         tgtAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACgctggc                                                                   <  2:177324/150‑1 (MQ=255)
                                                                             gATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGag                                                               <  1:742272/150‑1 (MQ=255)
                                                                             gATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGag                                                               >  1:129262/1‑150 (MQ=255)
                                                                              aTCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGagc                                                              >  1:54265/1‑150 (MQ=255)
                                                                               tCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGagca                                                             <  2:301875/150‑1 (MQ=255)
                                                                                   gTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCaa                                                         >  2:3335/1‑150 (MQ=255)
                                                                                    tAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAg                                                        >  1:109752/1‑150 (MQ=255)
                                                                                        ccTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGa                                                    >  2:511751/1‑150 (MQ=255)
                                                                                                  cGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTc                                          <  1:3335/150‑1 (MQ=255)
                                                                                                     cAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTc                                       <  1:511751/150‑1 (MQ=255)
                                                                                                       ttcTGAGGTTGTCCAGCGGTACCTCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCCTAAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGa                                     <  2:180996/148‑1 (MQ=255)
                                                                                                             ggTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTACAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGATCAGCAAGCCGATGCGGCTGTCTTCGATCATGt                               >  1:81879/1‑150 (MQ=255)
                                                                                                              gTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTa                              <  2:54265/150‑1 (MQ=255)
                                                                                                                    cAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAg                        >  2:367793/1‑150 (MQ=255)
                                                                                                                     aGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAgg                       <  2:842410/150‑1 (MQ=255)
                                                                                                                          tGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTc                  <  2:808802/150‑1 (MQ=255)
                                                                                                                                     atgccgccAGTGTGGCGGCATCGTCTTCCAGGCTGCAGCTGGTCAGGCCTTCCGGCAGCTAGCCCAGACTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATACGGCTGGCTTCGATCATATAGCCCAGGCGGTCGAGCGGGTACt       >  2:162478/3‑150 (MQ=255)
                                                                                                                                      tgccgccAGCCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGCGGGTACTc      >  1:805644/2‑150 (MQ=255)
                                                                                                                                        ccgccAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCCACCCATTCCTTGTGAGCAGCAAGCCGAGGCGGCTGTCTTCGATTATGTAGCGCAAGCGGTCGAGCGGGTACTCgg    >  1:444482/1‑150 (MQ=255)
                                                                                                                                          gccAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGAGCATGTAGCGCAGGCGGTCGAGCGGTTACTCgggg  <  2:288661/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
ACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGCGGGTACTCGGGG  >  NC_002947/4824174‑4824461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑