Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,848,199 G→A 13.8% L81L (CTG→CTA) ‡ PP_4263 → cytochrome biogenesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,848,1990GA13.8% 84.4 / 5.1 36L81L (CTG→CTA) ‡PP_4263cytochrome biogenesis protein
Reads supporting (aligned to +/- strand):  ref base G (12/19);  new base A (3/2);  total (15/21)
Fisher's exact test for biased strand distribution p-value = 6.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

ACTAACCTTGGCCATCCCCCCCGAACAACGCGGCAAGCGCCTGCGCCTGTTGCTGGCGTACAACCTGGGGCGCATTTTCAGCTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCACTGCTGCTGATTGCCATGGGCCTGTACCTGGCCGGCTGGTGGAGCGGGCTGACCCGTATCGAGGCACTGGGCCGCGGGCTGTGGCGGCATATCCAGCCGGTGGCGTCGCGATTGCTGC  >  NC_002947/4848052‑4848338
                                                                                                                                                     |                                                                                                                                           
aCTAACCTTGGCCATCCCCCCCGAACAACGCGGCAAGCGCCTGCGCCTGTTGCTGGCGTACAACCTGGGGCGCATTTTCAGCTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAg‑cgctggc                                                                                                                                           >  2:660776/1‑150 (MQ=255)
     ccTTGCCCATCCCACCCGAACAACGCGGCAAGGGCCTGCGCCTGTTGCTGGCGTACAACCTAGGGCGCATTTTCAGCTATGCGTGCTCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGACGTGGCCAACAGCCCGGCAG‑CGCTGGGGCTGc                                                                                                                                      <  2:177007/150‑1 (MQ=255)
      cTTGGCCATCCCCCCCGAACAACGCGGCAAGCGCCTGCGCCTGTTGCTGGCGTACAACCTGGGGCGCATTTTCAGCTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCCAGCGCTGcc                                                                                                                                     >  1:592303/1‑149 (MQ=255)
         ggCCATCCCCCCCGAACAACGCGGCAAGCGCCTGCGCCTGTTGCTGGCGTACAACCTGGGGCGCATTTTCAGCTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGggt                                                                                                                                  <  1:823160/150‑1 (MQ=255)
         ggCCATCCCCCCCGAACAACGCGGCAAGCGCCTGCGCCTGTTGCTGGCGTACAACCTGGGGCGCATTTTCAGCTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCCAGCGCTGCcggg                                                                                                                                  <  2:592303/150‑2 (MQ=255)
            cATCCCCCCCGAACAACGCGGCAAGCGCCTGCGCCTGTTGCTGGCGTACAACCTGGGGCGCATTTTCAGCTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGggtggt                                                                                                                               >  2:853180/1‑150 (MQ=255)
                  ccccGAACAACGCGGCAAGCGCCTGCGCCTGTTGCTGGCGTACAACCTGGGGCGCATTTTCAGCTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGc                                                                                                                         >  2:190705/1‑150 (MQ=255)
                  ccccGAACAACGCGGCAAGCGCCTGCGCCTGTTGCTGGCGTACAACCTGGGGCGCATTTTCAGCTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGc                                                                                                                         >  2:530799/1‑150 (MQ=255)
                  ccccGAACAACGCGGCAAGCGCCTGCGCCTGTTGCTGGCGTACAACCTGGGGCGCATTTTCAGCTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGCGTGGTCGCGGc                                                                                                                         >  2:601759/1‑150 (MQ=255)
                    ccGAACAACGCGGCAAGCGCCTGCGCATGTTGCTAGCGTACAACCTGGGGCGCATTTTCAGCTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCAc                                                                                                                       >  1:810302/1‑150 (MQ=255)
                          aacGCGGCAAGCGCCTGCGCCTGTTGCAGGCGTAAAACCTGGGGCCCATTTTCAGCTATGCCTGCGCCGGCCTGTTGCTTGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCActgctgc                                                                                                                 <  2:180620/150‑1 (MQ=255)
                             gcgGCAAGCGCCTGCGCCTGTTGCTGGCGTACAACCTGGGGCGCATTTTCAGCTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCACTGCTGCTGa                                                                                                              <  1:766139/150‑1 (MQ=255)
                                       cctgcgcctgTTGCTGGCGTACAACCTGGGGCGCCTTTTCCGCTATGCCTTCGCCGGCCGGTTGCTGGGCCT‑GCCGCTTGGGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCACTGCTGCTGATTGCCATggg                                                                                                    <  1:405832/150‑1 (MQ=255)
                                        ctgcgcctgTTGCTGGCGTACAACCTGGGGCGCATTTTCAGCTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCACTGCTGCTGATTGCCATGGGc                                                                                                   <  1:853180/150‑1 (MQ=255)
                                        ctgcgcctgTTGCTGGCGTACAACCTGGGGCGCATTTTCAGCTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCACTGCTGCTGATTGCCATGGGc                                                                                                   <  1:530799/150‑1 (MQ=255)
                                          gcgcctgTTGCTGGCGTACAACCTGGGGCGCATTTTCAGCTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCACTGCTGCTGATTGCCATGGGCCt                                                                                                 <  1:483262/150‑1 (MQ=255)
                                                 ttGCTGGCGTACAACCTGGGCCTCACCTTCATCGATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCACTGCTGCTGATTGCCATGGGCCTGTACCTg                                                                                          <  1:402914/150‑1 (MQ=255)
                                                     tGGCGTACAACCTGGGGCGCATTTTCAGCTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCACTGCTGCTGATTGCCATGGGCCTGTACCTGGCCg                                                                                      >  1:733528/1‑150 (MQ=255)
                                                     tGGCGTACAACCTGGGGCGCATTTTCAGCTATGCCCGCGCAGGCGTGTGGCTGGGCGGGCCCGGCT‑GTGGCGTGGCCTGCAG‑CCGGAAGACGGCAGCGGTGCTGGTGGTCGCGGCACTGCTGCTGATTGCCATGGACCTGTTCGTGGCCg                                                                                      >  1:619367/1‑150 (MQ=255)
                                                               ccTGGGGCGCATTTTCAGCTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCACTGCTGCTGATTGCCATGGGCCTGTACCTGGCCGGCTGGTGGAg                                                                            >  1:420942/1‑150 (MQ=255)
                                                                 tGGGGCGCATTTTCAGCTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCACTGCTGCTGATTGCCATGGGCCTGTACCTGGCCGGCTGGTGGAGCg                                                                          >  1:669768/1‑150 (MQ=255)
                                                                    ggCGCATTTTCAGCTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCACTGCTGCTGATTGCCATGGGCCTGTACCTGGCCGGCTGGTGGAGCGGGc                                                                       <  1:660776/150‑1 (MQ=255)
                                                                       gcATTTTCAGCTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCACTGCTGCTGATTGCCATGGGCCTGTACCTGGCCGGCTGGTGGAGCGGGCTGa                                                                    <  2:810302/150‑1 (MQ=255)
                                                                        cATTTTCAGCTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCACTGCTGCTGATTGCCATGGGCCTGTACCTGGCCGGCTGGTGGAGCGGGCTGAc                                                                   <  1:460239/150‑1 (MQ=255)
                                                                         aTTTTCAGCTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCAc                                                                                                                       >  1:121271/1‑97 (MQ=255)
                                                                         aTTTTCAGCTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCAc                                                                                                                       <  2:121271/97‑1 (MQ=255)
                                                                             tCAGCTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCACTGCTGCTGATTGCCATGGGCCTGTACCTGGCCGGCTGGTGGAGCGGGCTGACCCGTa                                                              <  2:371433/150‑1 (MQ=255)
                                                                                 cTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCACTGCTGCTGATTGCCATGGGCCTGTACCTGGCCGGCTGGTGGAGCGGGCTGACCCGTATCGa                                                          <  1:748935/150‑1 (MQ=255)
                                                                                       cTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCACTGCTGCTGATTGCCATGGGCCTGTACCTGGCCGGCTGGTGGAGCGGGCTGACCCGTATCGAGGCACt                                                    >  1:92951/1‑150 (MQ=255)
                                                                                           gcCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCACTGCTGCTGATTGCCATGGGCCTGTACCTGGCCGGCTGGTGGAGCGGGCTGACCCGt                                                               <  1:769380/135‑1 (MQ=255)
                                                                                           gcCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCACTGCTGCTGATTGCCATGGGCCTGTACCTGGCCGGCTGGTGGAGCGGGCTGACCCGt                                                               >  2:769380/1‑135 (MQ=255)
                                                                                                          ggCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCACTGCTGCTGATTGCCATGGGCCTGTACCTGGCCGGCTGGTGGAGCGGGCTGACCCGTATCGAGGCACTGGGCCGCGGGCTGTggcgg                                 <  2:420942/150‑1 (MQ=255)
                                                                                                           gCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCACTGCTGCTGATTGCCATGGGCCTGTACCTGGCCGGCTGGTGGAGCGGGCTGACCCGTATCGAGGCACTGGGCCGCGGGCTGTggcggc                                <  2:523026/150‑1 (MQ=255)
                                                                                                               ggCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCACTGCTGCTGATTGCCATGGGCCTGTACCTGGCCGGCTGGTGGAGCGGGCTGACCCGTATCGAGGCACTGGGCCGCGGGCTGTGGCGGCatat                            >  2:633291/1‑150 (MQ=255)
                                                                                                                                          ccgCAG‑CGCCAGCGCTGCGGGTGGTCGCGGCACTGCTGCTGATTGCCATGGGCCTGTACCTGGCCGGCTGGTGGAGCGGGCTGACCCGTATCGAGGCACTGGGCCGCGGGCTGTGGCGGCat                              <  2:344641/120‑1 (MQ=255)
                                                                                                                                          ccgCAG‑CGCCAGCGCTGCGGGTGGTCGCGGCACTGCTGCTGATTGCCATGGGCCTGTACCTGGCCGGCTGGTGGAGCGGGCTGACCCGTATCGAGGCACTGGGCCGCGGGCTGTGGCGGCat                              >  1:344641/3‑122 (MQ=255)
                                                                                                                                          cGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCACTGCTGCTGATTGCCATGGGCCTGTACCTGGCCGGCTGGTGGAGCGGGCTGACCCGTATCGAGGCACTGGGCCGCGGGCTGTGGCGGCATATCCAGCCGGTGGCGTCGCGATtgctgc  <  1:633291/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                           
ACTAACCTTGGCCATCCCCCCCGAACAACGCGGCAAGCGCCTGCGCCTGTTGCTGGCGTACAACCTGGGGCGCATTTTCAGCTATGCCTGCGCCGGCCTGTTGCTGGGCCTGGCCGGCT‑GGGCCGTGGCCAGCAGCCCGGCAG‑CGCTGGCGCTGCGGGTGGTCGCGGCACTGCTGCTGATTGCCATGGGCCTGTACCTGGCCGGCTGGTGGAGCGGGCTGACCCGTATCGAGGCACTGGGCCGCGGGCTGTGGCGGCATATCCAGCCGGTGGCGTCGCGATTGCTGC  >  NC_002947/4848052‑4848338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑