Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 384,786 C→G 11.6% R421G (CGC→GGC)  PP_0320 → methyl‑accepting chemotaxis transducer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947384,7860CG11.6% 118.6 / 8.6 43R421G (CGC→GGC) PP_0320methyl‑accepting chemotaxis transducer
Reads supporting (aligned to +/- strand):  ref base C (20/18);  new base G (3/2);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.77e-01

GAGTACCTCCGAGCGGCTGCGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTCAGCCAGCTGGCCGA  >  NC_002947/384638‑384934
                                                                                                                                                    |                                                                                                                                                    
gAGTACCTCCGAGCGGCTGCGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCg                                                                                                                                                     <  2:119643/150‑1 (MQ=255)
       tCCGAGCGGCTGCGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGcc                                                                                                                                              >  2:291471/1‑150 (MQ=255)
             cGGCTGCGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCGGCATTGCGGGCGAt                                                                                                                                        <  2:195629/150‑1 (MQ=255)
                       gggCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAgg                                                                                                                              >  2:296130/1‑150 (MQ=255)
                                          ccAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCacgaga                                                                                                           >  1:470697/1‑150 (MQ=255)
                                          ccAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACAGTAACGCGGCGCGCGCGCAAAGCCGGCGATGCGGGCCAGGCGTCGCAGTCTGCacgaga                                                                                                           >  2:239779/1‑150 (MQ=255)
                                            aGGTGGTGGACAACACCGCCGAGCGCTCCGCGTGTCCTCTTCAGATATCCGGAATGGTCGCAACTGCTGTACCTGGGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCacgagacg                                                                                                         <  1:615718/150‑1 (MQ=255)
                                             ggtggtGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCacgagacga                                                                                                        <  2:470697/150‑1 (MQ=255)
                                                  tggACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGt                                                                                                   >  1:819405/1‑150 (MQ=255)
                                                    gACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCTCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTg                                                                                                 <  1:485414/150‑1 (MQ=255)
                                                     acaacaCCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGc                                                                                                <  1:49711/150‑1 (MQ=255)
                                                       aacaCCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAg                                                                                              >  2:96097/1‑150 (MQ=255)
                                                             gccgAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGt                                                                                                                     >  1:622916/1‑121 (MQ=255)
                                                             gccgAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGt                                                                                                                     <  2:622916/121‑1 (MQ=255)
                                                                 aGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCGCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAgg                                                                                    >  1:53955/1‑150 (MQ=255)
                                                                 aGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAgg                                                                                    <  2:560171/150‑1 (MQ=255)
                                                                    gcTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAggtgg                                                                                 >  1:308470/1‑150 (MQ=255)
                                                                             gcCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGCCGATCTTCATTGCGTAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGAt                                                                        <  2:450698/150‑1 (MQ=255)
                                                                                     gagaTGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGt                                                                <  1:96097/150‑1 (MQ=255)
                                                                                       gaTGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGTGAGGTGGTGCAACGATCCATTCGTgg                                                              <  1:117023/150‑1 (MQ=255)
                                                                                        aTGACCGAAATGGTCGCCACTGCTGTACACGAGATTGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGc                                                             >  2:388385/1‑150 (MQ=255)
                                                                                          gACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTc                                                                                                                    >  1:763161/1‑93 (MQ=255)
                                                                                          gACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTc                                                                                                                    <  2:763161/93‑1 (MQ=255)
                                                                                               aaaTGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAgg                                                      >  2:707600/1‑150 (MQ=255)
                                                                                                     tCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCacgagac                                                                                                          <  1:5995/92‑1 (MQ=255)
                                                                                                     tCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCacgagac                                                                                                          >  2:5995/1‑92 (MQ=255)
                                                                                                     tCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGt                                                >  1:301789/1‑150 (MQ=255)
                                                                                                       gCCACTGCTGTACACGAGATGGGCCTGACCATCGTTAACAACGCCCGCAATGCCGGCGATGCGGCCCAGGCGACGCATGTTACACGAGACGAGGCGTTGCAGGCGCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCg                                              >  1:496177/1‑150 (MQ=255)
                                                                                                        ccACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCgg                                                                                                                  >  2:554354/1‑81 (MQ=255)
                                                                                                        ccACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCgg                                                                                                                  <  1:554354/81‑1 (MQ=255)
                                                                                                          aCTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGc                                           <  2:53955/150‑1 (MQ=255)
                                                                                                            tgctgTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGa                                         >  1:352461/1‑150 (MQ=255)
                                                                                                                 tACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGc                                           >  1:197767/1‑143 (MQ=255)
                                                                                                                 tACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGc                                           <  2:197767/143‑1 (MQ=255)
                                                                                                                              ccTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGa                       >  1:741865/1‑150 (MQ=255)
                                                                                                                               cTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGAGATCGGCAAGGCGGCCGAt                      <  1:781869/150‑1 (MQ=255)
                                                                                                                                   ccGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCgg                  <  2:819405/150‑1 (MQ=255)
                                                                                                                                     gTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCGTGTCGGGCGACATCGGAAAGGCGGCCGATGCGGtt                >  1:359632/1‑149 (MQ=255)
                                                                                                                                      tGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTca               <  1:291471/150‑1 (MQ=255)
                                                                                                                                             cATCGCCGGCATTGCGGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGAGCGACATCGGCAAGGCGGCCGATGCGGTCAGCCAGCt        >  1:510947/1‑150 (MQ=255)
                                                                                                                                              aTCGCCGGCATTGCGGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTcagccagccg       >  1:761023/1‑148 (MQ=255)
                                                                                                                                               tCGCCGGCATTGCGGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTCAGCCAGCTgg      <  2:514999/150‑1 (MQ=255)
                                                                                                                                               tCGCCGGCATTGCGGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTCAGCCAGCTgg      >  1:514999/1‑150 (MQ=255)
                                                                                                                                                   ccGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTCAGCCAGCTGGCcga  <  1:296130/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAGTACCTCCGAGCGGCTGCGACGGGCCGTGGAGCAGGTAACCCAGGTGGTGGACAACACCGCCGAGCGCTCCGGGCGCCAGCAGGAGATGACCGAAATGGTCGCCACTGCTGTACACGAGATGGGCCTGACCGTGCAGGACATCGCCCGCAATGCCGGCGATGCGGCCCAGGCGTCGCAGTCGGCACGAGACGAGGCGTTGCAGGCCCGCGAGGTGGTGCAACGATCCATTCGTGGCATCGAGGGCATGTCGGGCGACATCGGCAAGGCGGCCGATGCGGTCAGCCAGCTGGCCGA  >  NC_002947/384638‑384934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑