Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,902,717 T→C 10.7% V196A (GTG→GCG) ‡ PP_4310 → hydantoin racemase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,902,7170TC10.7% 140.5 / 5.4 54V196A (GTG→GCG) ‡PP_4310hydantoin racemase
Reads supporting (aligned to +/- strand):  ref base T (30/18);  new base C (3/3);  total (33/21)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

CTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATGCGCTTCGGCCGCTAGAGAT  >  NC_002947/4902569‑4902863
                                                                                                                                                    |                                                                                                                                                  
ctcgaaatggacGCCGAACCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTg                                                                                                                                                   >  2:460364/4‑150 (MQ=255)
  ggaactggaaGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGcc                                                                                                                                                 >  2:695304/1‑150 (MQ=255)
         gaaGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGgtggtg                                                                                                                                          >  2:638884/1‑150 (MQ=255)
         gaaGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGgtggtg                                                                                                                                          >  1:801921/1‑150 (MQ=255)
          aaGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCACGCCggtgcgtt                                                                                                                                         <  1:156472/150‑5 (MQ=255)
             ccGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATg                                                                                                                                      >  1:27867/1‑150 (MQ=255)
                    gCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAg                                                                                                                               >  1:640531/1‑150 (MQ=255)
                      tGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTg                                                                                                                             >  2:630050/1‑150 (MQ=255)
                            ccGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCgg                                                                                                                       >  2:291019/1‑150 (MQ=255)
                             cGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGt                                                                                                                      <  1:695304/150‑1 (MQ=255)
                                                    ccGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTgc                                                                                               >  2:428402/1‑150 (MQ=255)
                                                    ccGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTATGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGc                                                                                                             <  2:789161/136‑1 (MQ=255)
                                                    ccGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTATGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGc                                                                                                             >  1:789161/1‑136 (MQ=255)
                                                            gcCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGt                                                                                       >  1:427587/1‑150 (MQ=255)
                                                                    cgacgaCAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCAc                                                                               <  1:291019/150‑1 (MQ=255)
                                                                     gacgacAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACg                                                                              >  1:23978/1‑150 (MQ=255)
                                                                           aaGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCaaa                                                                        >  1:155718/1‑150 (MQ=255)
                                                                           aaGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCaaa                                                                        <  1:352592/150‑1 (MQ=255)
                                                                                 gAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTgcgc                                                                  <  2:640531/150‑1 (MQ=255)
                                                                                 gAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTgcgc                                                                  >  2:808565/1‑150 (MQ=255)
                                                                                  aGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCa                                                                 <  1:808565/150‑1 (MQ=255)
                                                                                       aTCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTAt                                                            <  1:630050/150‑1 (MQ=255)
                                                                                            ccTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTg                                                                                                   <  1:721193/106‑1 (MQ=255)
                                                                                            ccTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTg                                                                                                   >  2:721193/1‑106 (MQ=255)
                                                                                            ccTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGAc                                                       >  1:562449/1‑150 (MQ=255)
                                                                                            ccTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGAc                                                       >  1:323563/1‑150 (MQ=255)
                                                                                            ccTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGAc                                                       >  2:536080/1‑150 (MQ=255)
                                                                                             cTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACg                                                      >  2:35183/1‑150 (MQ=255)
                                                                                                ggTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGgtgt                                                                                                                                  <  2:168308/71‑1 (MQ=255)
                                                                                                ggTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGCGGATGgtgt                                                                                                                                  >  1:168308/1‑71 (MQ=255)
                                                                                                     tgGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGTTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACgg                                                <  1:458708/148‑1 (MQ=255)
                                                                                                     tgGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGTTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACgg                                                >  2:458708/1‑148 (MQ=255)
                                                                                                     tgGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGc                                              <  2:531436/150‑1 (MQ=255)
                                                                                                      ggcggcATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCg                                             <  1:460364/150‑1 (MQ=255)
                                                                                                       gcggcATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCga                                            >  1:665791/1‑150 (MQ=255)
                                                                                                            aTGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGaaa                                       >  2:296882/1‑150 (MQ=255)
                                                                                                            aTGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGaaa                                       >  1:367995/1‑150 (MQ=255)
                                                                                                                  gggCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAgtggtg                                 <  2:647750/150‑1 (MQ=255)
                                                                                                                    gCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTggg                               <  2:155718/150‑1 (MQ=255)
                                                                                                                     cTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGc                              <  2:801921/150‑1 (MQ=255)
                                                                                                                      tGGACGAGCAGATTCGCCACCGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCTTTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAgtg                                    <  1:762698/143‑1 (MQ=255)
                                                                                                                      tGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCTTTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAgtg                                    >  2:762698/1‑143 (MQ=255)
                                                                                                                               aGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATgc                    >  2:802693/1‑150 (MQ=255)
                                                                                                                                 aTTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGGAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATgcgc                  >  2:618438/1‑150 (MQ=255)
                                                                                                                                     gCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATGCGCTTCg              >  2:320658/1‑150 (MQ=255)
                                                                                                                                      ccAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATGCGCTTCgg             >  2:178149/1‑150 (MQ=255)
                                                                                                                                      atccaccACCGGCACGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATGCGCTTCgg             >  2:514152/7‑150 (MQ=255)
                                                                                                                                          cGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATGCGCTTCGGCCGc         <  2:562449/150‑1 (MQ=255)
                                                                                                                                          accACCGGCACGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAgtg                                    <  2:579138/121‑1 (MQ=255)
                                                                                                                                          accACCGGCACGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAgtg                                    >  1:579138/3‑123 (MQ=255)
                                                                                                                                            cACCGGCACGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATGCGCTTCGGCCGCTa       >  1:150788/1‑150 (MQ=255)
                                                                                                                                              ccGGCACGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATGCGCTTCGGCCGCTaga     <  2:150788/150‑1 (MQ=255)
                                                                                                                                                ggCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATGCGCTTCGGCCGCTAgaga   <  2:665791/150‑1 (MQ=255)
                                                                                                                                                 gCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATGCGCTTCGGCCGCTAGAGAt  >  2:842163/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGGGTTTGAGCACGTCCAAAGTGCGCACCTATGCGACGCCACGGGCGAAGAAAGTGGTGGGCTGGCCGATGCGCTTCGGCCGCTAGAGAT  >  NC_002947/4902569‑4902863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑