Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,931,245 C→T 17.1% G57D (GGC→GAC)  cheY ← two‑component system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,931,2450CT17.1% 89.0 / 12.4 41G57D (GGC→GAC) cheYtwo‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base C (16/18);  new base T (3/4);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.11e-01

GCTTGACCACATAACCATTGACGCCAGCCTGGGCCGCTTCAATGATCTGGTCGCGCTTGGCTTCAGCGGTCACCATCAAGACCGGCATGCTTTTCAGCTTGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGTTTTCCAGCATCGGCAACGCCGTGGTGCCGTCATCGGCTTCGTCGGTGTTGGTGAAACCCAGGTCACGCAACAGGTTCTTGATGATCCGCCGCATCGTCGAAAAG  >  NC_002947/4931102‑4931391
                                                                                                                                               |                                                                                                                                                  
gCTTGACCACATAACCATTGACGCCAGCCTGGGCCGCTTCAATGATCTGGTCGCGCTTGGCTTCAGCGGTCACCATCAAGACCGGCATGCTTTTCAGCTTGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCa                                                                                                                                              <  2:120199/150‑1 (MQ=255)
gCTTGACCACATAACCATTGACGCCAGCCTGGGCCGCTTCAATGATCTGGTCGCGCTTGGCTTCAGCGGTCACCATCAAGACCGGCATGCTTTTCAGCTTGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCa                                                                                                                                              <  1:415143/150‑1 (MQ=255)
 cTTGACCACATAACCATTGACGCCAGCCTGGGCCGCTTCAATGATCTGGTCGCGCTTGGCTTCAGCGGTCACCATCAAGACCGGCATGCTTTTCAGCTTGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGGGCATGTCCGGCAt                                                                                                                                             <  2:484973/150‑1 (MQ=255)
        acaTAACCATTGACGCCAGCCTGGGCCGCTTCAATGATCTGGTCGCGCTTGGCTTCAGCGGTCACCATCAAGACCGGCATGCTTTTCAGCTTGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTc                                                                                                                                         <  2:711832/147‑1 (MQ=255)
        acaTAACCATTGACGCCAGCCTGGGCCGCTTCAATGATCTGGTCGCGCTTGGCTTCAGCGGTCACCATCAAGACCGGCATGCTTTTCAGCTTGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTc                                                                                                                                         >  1:711832/1‑147 (MQ=255)
          aTAACCATTGACGCCAGCCTGGGCCGCTTCAATGATCTGGTCGCGCTTGGCTTCAGCGGTCACCATCAAGACCGGCATGCTTTTCAGCTTGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTCCAGTc                                                                                                                                    <  2:729037/150‑1 (MQ=255)
            aaCCATTGACGCCAGCCTGGGCCGCTTCAATGATCTGGTCGCGCTTGGCTTCAGCGGTCACCATCAAGACCGGCATGCTTTTCAGCTTGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGCACATGCACGGCATGTTCCAGTCgg                                                                                                                                  >  2:375044/1‑150 (MQ=255)
                 ttGACGCCAGCCTGGGCCGCTTCAATGATCTGGTCGCGCTTGGATCAACAGGTCACCATCAAGACCGGCATGCTTTTCAGCTTGTCGCTGGCACGAACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTCCAGTCGGTCAcc                                                                                                                             >  1:794424/1‑150 (MQ=255)
                                  cGCTTCAATGATCTGGGCGCGCTTGGTTTCAGCGGTCACCATCAAATCCGGTATGATTTTCAACTTGTCGCTGGCACGCACTTTACCCAGCAGGTCGATGCCGGACATGCACGGCCTGTTCCAGTCGGTCACCAGGAAGTCGTAGTGAcc                                                                                                            <  1:375044/150‑1 (MQ=255)
                                         aTGATCTGGTCGCCATTGACTTCAACGGTCACCATCAAGACCGGCATGCTTTTCAGCTTGTCGCTGTCACGCACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGTTTTcc                                                                                                     <  2:394817/150‑1 (MQ=255)
                                          tGATCTGGTCGCGCTTGGCTTCAGCGGTCACCATCAAGACCGGCATGCTTTTCAGCTTGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTa                                                                                                                  >  1:522166/1‑136 (MQ=255)
                                          tGATCTGGTCGCGCTTGGCTTCAGCGGTCACCATCAAGACCGGCATGCTTTTCAGCTTGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTa                                                                                                                  <  2:522166/136‑1 (MQ=255)
                                                            cTTCAGCGGTCACCATCAAGACCGGCATGCTTTTCAGCTTGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAg                                                                                                                 >  2:790047/1‑119 (MQ=255)
                                                            cTTCAGCGGTCACCATCAAGACCGGCATGCTTTTCAGCTTGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAg                                                                                                                 <  1:790047/119‑1 (MQ=255)
                                                             ttCAGCGGTCACCATCAAGACCGGCATGCTTTTCAGCTTGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGTTTTCCAGCATCGGCAACGCCgtggt                                                                                 >  1:638538/1‑150 (MQ=255)
                                                                       aCCATCAAGACCGGCATGCTTTTCAGCTTGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGTTTTcc                                                                                                     >  2:2544/1‑120 (MQ=255)
                                                                       aCCATCAAGACCGGCATGCTTTTCAGCTTGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGTTTTcc                                                                                                     <  1:2544/120‑1 (MQ=255)
                                                                          aTCAAGACCGGCATGCTTTTCAGCTTGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGTTTTCCAGCATCGGCAACGCCGTGGTGCCGTCATCGGCt                                                                    >  2:232122/1‑150 (MQ=255)
                                                                               gACCGGCATGCTTTTCAGCTTGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGTTTTCCAGCATCGGCAACGCCGTGGTGCCGTCATCGGCTtcgtc                                                               <  2:95294/150‑1 (MQ=255)
                                                                                           tttCAGCTTGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTCCAGTc                                                                                                                                    >  1:750266/1‑69 (MQ=255)
                                                                                           tttCAGCTTGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTCCAGTc                                                                                                                                    <  2:750276/69‑1 (MQ=255)
                                                                                           tttCAGCTTGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTCCAGTc                                                                                                                                    <  2:750266/69‑1 (MQ=255)
                                                                                           tttCAGCTTGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTCCAGTc                                                                                                                                    >  1:750276/1‑69 (MQ=255)
                                                                                             tCAGCTTGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGTTTTCCAGCATCGGCAACGCCGTGGTGCCGTCATCGGCTTCGTCGGTGTTGGTGaaca                                                 <  2:638538/150‑3 (MQ=255)
                                                                                               aGCTTGTCGCTGGCACGGGTTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGTTTTCCAGCATCGGCAACGCCGTGGTGCCGTCATCGGCTTCGTCGGTGTTGGTGAAAccc                                               <  2:794424/150‑1 (MQ=255)
                                                                                                  ttGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGt                                                                                                          >  2:29211/1‑88 (MQ=255)
                                                                                                  ttGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGt                                                                                                          <  1:29211/88‑1 (MQ=255)
                                                                                                             acgcacTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGTTTTCCAGCATCGGCAACGCCGTGGTGCCGTCATCGGCTTCGTCGGTGTTGGTGAAACCCAGGTCACGCAACAg                                 >  2:489846/1‑150 (MQ=255)
                                                                                                               gcacTTTACGCAGCAGGTCGATGCCGGGCATGTCCGGCAtc                                                                                                                                            >  1:489988/1‑40 (MQ=21)
                                                                                                               gcacTTTACGCAGCAGGTCGATGCCGGGCATGTCCGGCAtc                                                                                                                                            <  2:489988/41‑2 (MQ=21)
                                                                                                                               gaacaTGCCGGGCATGTCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGTTTTCCAGCATCGGCAACGCCGTGGTGCCGTCATCGGCTTCGTCGGTGTTGGTGAAACCCAGGTCACGCAACAGGTTc                             >  1:391893/5‑136 (MQ=255)
                                                                                                                               gaacaTGCCGGGCATGTCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGTTTTCCAGCATCGGCAACGCCGTGGTGCCGTCATCGGCTTCGTCGGTGTTGGTGAAACCCAGGTCACGCAACAGGTTc                             <  2:391893/132‑1 (MQ=255)
                                                                                                                               gTCGATGCCGGACATGCCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGTTTTCCAGCATCGGCAACGCCGTGGTGCCGTCATCGGCTTCGTCGGTGTTGGTGaaa                                                  >  2:677587/1‑115 (MQ=255)
                                                                                                                               gTCGATGCCGGACATGCCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGTTTTCCAGCATCGGCAACGCCGTGGTGCCGTCATCGGCTTCGTCGGTGTTGGTGaaa                                                  <  1:677587/115‑1 (MQ=255)
                                                                                                                                  gATGCCGGACATGCCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGTTTTCCAGCATCGGCAACGCCGTGGTGCCGTCATCGGCTTCGTCGGTGTTGGTGAAACCCAGGTCACGCAACAGGTTCTTGATGATCCGCCGCAt            >  1:290244/1‑150 (MQ=255)
                                                                                                                                   aTGCCGGGCATGTCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGTTTTCCAGCATCGGCAACGCCGTGGTGCCGTCATCGGCTTCGTCGGTGTTGGTGAAACCCAGGTCACGCAACAGGTTCTTGATGATCCGCCGCAtc           >  2:259072/1‑150 (MQ=255)
                                                                                                                                      ccGGACATGCCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGTTTTCCAGCATCGGCAACGCCGTGGTGCCGTCATCGGCTTCGTCGGTGTTGGTGAAACCCAGGTCACGCAACAGGTTCTTGATGATCCGCCGCAtcgtc        <  2:290244/150‑1 (MQ=255)
                                                                                                                                       cGGACATGCCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGTTTTCCAGCATCGGCAACGCCGTGGTGCCGTCATCGGCTTCGTCGGTGTTGGTGAAACCCAGGTCACGCAACAGGTTCTTGATGATCCGCCGCAtcgtcg       >  1:366101/1‑150 (MQ=255)
                                                                                                                                          aCATGCCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGTTTTCCAGCATCGGCAACGCCGTGGTGCCGTCATCGGCTTCGTCGGTGTTg                                                        <  2:426894/98‑1 (MQ=255)
                                                                                                                                          aCATGCCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGTTTTCCAGCATCGGCAACGCCGTGGTGCCGTCATCGGCTTCGTCGGTGTTg                                                        >  1:426894/1‑98 (MQ=255)
                                                                                                                                           cATGTCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGTTTTCCAGCATCGGCAACGCCGTGGTGCCGTCATCGGCTTCGTCGGTGTTGGTGAAACCCAGGTCACGCAACAGGTTCTTGATGATCCGCCGCATCGTCGaaaa   <  1:259072/150‑1 (MQ=255)
                                                                                                                                            aatcccGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGTTTTCCAGCATCGGCAACGCCGTGGTGCCGTCATCGGCTTCGTCGGTGTTGGTGAAACCCAGGTCACGCAACAGGTTCTTGATGATCCGCCGCATCGTCGAAAAg  <  1:723749/147‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
GCTTGACCACATAACCATTGACGCCAGCCTGGGCCGCTTCAATGATCTGGTCGCGCTTGGCTTCAGCGGTCACCATCAAGACCGGCATGCTTTTCAGCTTGTCGCTGGCACGCACTTTACGCAGCAGGTCGATGCCGGACATGCCCGGCATGTTCCAGTCGGTCACCAGGAAGTCGTAGTGACCGTTTTCCAGCATCGGCAACGCCGTGGTGCCGTCATCGGCTTCGTCGGTGTTGGTGAAACCCAGGTCACGCAACAGGTTCTTGATGATCCGCCGCATCGTCGAAAAG  >  NC_002947/4931102‑4931391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑