| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,015,961 | A→T | 11.1% | intergenic (‑176/+58) | PP_5679 ← / ← PP_4421 | hypothetical protein/aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,015,961 | 0 | A | T | 11.1% | 100.5 / 5.2 | 36 | intergenic (‑176/+58) | PP_5679/PP_4421 | hypothetical protein/aminotransferase |
| Reads supporting (aligned to +/- strand): ref base A (15/17); new base T (2/2); total (17/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01 | |||||||||||
GATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGTGTGCGATGCTGATGATGCGATCCACTTCCTGTTCGGTCGTGACAAGA > NC_002947/5015815‑5016106 | gatgaATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGTTAGCACTAC‑AAAAAAAAAAAGCCCGGCTAAAgg > 1:394529/1‑150 (MQ=255) atgaATAAGCCCGCACCGGTCTCCGGCGGGGTCTTTTACGTTCGGTGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAAGCCCGCCTAAAGGc > 1:19264/1‑150 (MQ=255) ccGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTaaaa > 1:156232/1‑150 (MQ=255) ccGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTaaaa > 1:160349/1‑150 (MQ=255) aCCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGaa < 1:565746/150‑1 (MQ=255) ggtacccGGCGCGGGCTTTAACTTTCCGGGCCTTGGACAGAATCTGACCCGAAAGTACGTCCTTGTGAAATGATGACAGCGTCTTGGCTTTGATTGTGATAGAAATAC‑AAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAagag < 2:393293/145‑1 (MQ=255) cgGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGc < 1:605154/150‑1 (MQ=255) tttCGGGGCCTTGGACAGTATCTGAACGGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTAATAGCAATAC‑AAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTg < 2:730349/150‑1 (MQ=255) gCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAAGCCCGCCTAAAGGCGGGCCTAAAACGAAGAGGGAAATTCAGCTGGCATCATTGCCaaaga > 1:819726/1‑147 (MQ=255) ggACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTTTTGGGTTTCATCGGAAGAGGACGAC‑AAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATg < 1:101520/150‑1 (MQ=255) gACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAgg > 2:237524/1‑116 (MQ=255) gACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAgg < 1:237524/116‑1 (MQ=255) gACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATgg < 1:136595/150‑1 (MQ=255) aCAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGc < 2:19264/150‑1 (MQ=255) ccGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAAAGGGTAATTCAGCTGGTATCATTGCCAACGTTTATGGTTCAGACCTTCaa > 1:309585/1‑149 (MQ=255) aaCGTCCTTGTGAACTAAAGAAATCGTCTTGGCTTTGATTGTAATAAAAAAAA‑AAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGATGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCtcatca < 2:160349/149‑1 (MQ=255) ttGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGc > 1:767278/1‑150 (MQ=255) gaCAGCGTCTTGGCTTTGATTGTGCTAGCACTAC‑AAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCtt > 1:240733/1‑150 (MQ=255) aGCGTCTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAAGCCCGCCTTTAGGCGGGCtt < 1:439267/63‑1 (MQ=25) aGCGTCTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAAGCCCGCCTTTAGGCGGGCtt > 2:439267/1‑63 (MQ=25) cGTCTTGGCTTAGAAAGTGCTCGACATAG‑AACACAACAAAGCCCGCCTAAATGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCgtgt < 2:309585/150‑1 (MQ=255) cTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGTGTGc < 1:3062/150‑1 (MQ=255) cTTGGCTTTGATTGTGATAGCACTACAAAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGTGTGc > 2:3062/1‑150 (MQ=255) tttGATTGTGATAGCACTAC‑AAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAgg > 1:182199/1‑63 (MQ=255) tttCATTGTGATAGCACTAC‑AAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAgg < 2:182199/63‑1 (MQ=255) tGATTGTGATAGCACTAC‑AAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGTGTGCGATGCtgat < 2:240733/150‑1 (MQ=255) aTAGCACTACAAAAAAAAAAAAGCCCCCCTAAAGGCGGGCTTAAAACAAAAAGGGTAATTCACCTGGCACCATTGCCAACGTTGATGGCTCAGACCTTCACCCCATCAAGAACCCCCACCCAACCTTCGTGTCGGTTGTTGATGAGGCGa > 2:680696/1‑150 (MQ=255) cACTAC‑AAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCaa > 1:633663/1‑104 (MQ=255) cACTAC‑AAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCaa < 2:633663/104‑1 (MQ=255) aaaaaaaaaGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGTGTGCGATGCTGATGATGCGATCCACTTCCTGtt < 2:767278/150‑1 (MQ=255) aaaaaaaGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGTGTGCGATGCTGATGATGCGATCCACTTCCTGTTCg < 1:680696/150‑1 (MQ=255) gCCCGCCTTTAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGTGTGCGATGCTGATGATGCGATCCACTTCCTGTTCGGTc < 2:403999/146‑1 (MQ=255) gCCCGCCTTTAGGCGGGCTTAAAACGAAGAGGGCAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGTGTGCGATGCTGATGATGCGATCCACTTCCTGTTCGGTc > 1:403999/1‑146 (MQ=255) gCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGTGTGCGATGCTGATGATGCGATCCACTTc < 2:582221/132‑1 (MQ=255) gCCTAAAGGCGGGCTTAAAACGAAGAGGCTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGTGTGCGATGCTGATGATGCGATCCACTTc > 1:582221/1‑132 (MQ=255) ccTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGTGTGCGATGCTGATGATGCGATCCACTTCCTGTTCGGTCGTGACAaga > 2:369810/1‑150 (MQ=255) | GATGAATAAGCCCGCACCGGTCTCCGGCGCGGGCTTTTACTTTCCGGGCCTTGGACAGTATCTGACCCGAAAGTACGTCCTTGTGAACTGATGACAGCGTCTTGGCTTTGATTGTGATAGCACTAC‑AAAAAAAAAAAGCCCGCCTAAAGGCGGGCTTAAAACGAAGAGGGTAATTCAGCTGGCATCATTGCCAACGTTGATGGCTCAGACCTTCAGCTCATCAAGGACGCTCAGCACAGCTTCGTGTGCGATGCTGATGATGCGATCCACTTCCTGTTCGGTCGTGACAAGA > NC_002947/5015815‑5016106 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |