| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,077,270 | G→A | 16.2% | intergenic (+17/+91) | PP_4470 → / ← mgtE | Arc domain‑containing transcriptional regulator/magnesium transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,077,270 | 0 | G | A | 16.2% | 81.2 / 8.6 | 37 | intergenic (+17/+91) | PP_4470/mgtE | Arc domain‑containing transcriptional regulator/magnesium transporter |
| Reads supporting (aligned to +/- strand): ref base G (13/18); new base A (3/3); total (16/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
AGCCCTGAACTGTCCCTGCATGAGCGCGAGTTGCTGCAGCGTTTCCGCCAACTGTCCCACCGCCAGCAGAACGCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGTCTGACTGGCAGCAAGGCCATTTCCCTGCGGGATTGTTCCCACAGGGAGGATTGGCTTACAACAGGAACAGCGTGGCCAGCCCAAGGAAGA > NC_002947/5077128‑5077395 | aGCCCTGAACTGTCCCTGCATGAGCGCGAGTTGCTGCAGCGTTTCCGCCAACTGTCCCACCGCCAGCAGAACGCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGc < 1:685302/150‑1 (MQ=255) cTGCATGAGCGCGAGTTGCTGCAGCGTTTCCGCCAACTGTCCCACCGCCAGCAGAACGCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACAAAAAAGCCAGCGTAAGCTGGc < 2:351046/149‑1 (MQ=255) cTGCATGAGCGCGAGTTGCTGCAGCGTTTCCGCCAACTGTCCCACCGCCAGCAGAACGCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACAAAAAAGCCAGCGTAAGCTGGc > 1:351046/1‑149 (MQ=255) cATGAGCGCGAGTTGCTGCAGCGTTTCCGCCAACTGTCCCACCGCCAGCAGAACGCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCtttt > 1:454668/1‑150 (MQ=255) cATGAGCGCGAGTTGCTGCAGCGTTTCCGCCAACTGTCCCACCGCCAGCAGAACGCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCtttt < 2:669126/150‑1 (MQ=255) aTGAGCGCGAGTTGCTGCAGCGTTTCCGCCAACTGTCCCACCGCCAGCAGAACGCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCTTACGCTGGCttttt < 2:59584/150‑1 (MQ=255) tGAGCGCGAGTTGCTGCAGCGTTTCCGCCAACTGTCCCACCGCCAGCAGAACGCCTTGGTCGCCCTCATCGCTCACGATTCGGAAATGGCCGCAAACAACTGGCGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCtttttt < 2:120621/150‑1 (MQ=255) aGCGCGAGTTGCTGCAGCGTTTCCGCCAACTGTCCCACCGCCAGCAGAACGCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTtgt < 1:511242/150‑1 (MQ=255) cGCCAACTGTCCCACCGCCAGCAGAACGCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCTGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGTCTGACTGGCAGCAAGGc > 2:417820/1‑150 (MQ=255) cGCCAACTGTCCCACCGCCAGCAGAACGCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGACCACGAAAAAGTCAGCGTAAGCTGGCTTTTTTGTGGGCGTCTGACTGGCAGCAAGGc < 2:760151/150‑1 (MQ=255) cGCCAACTGTCCCACCGCCAGCAGAACGCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGTGTCTGACTGGCAGCAAGGc > 2:561931/1‑150 (MQ=255) cGCCAACTGTCCCACCGCCAGCAGAACGCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGTCTGACTGGCAGCAAGGc > 2:505078/1‑150 (MQ=255) gCCAACTGTCCCACCGCCAGCAGAACGCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACAAAAAAGCCAGCTTACGCTGGCtttttcgt > 2:346311/1‑123 (MQ=39) gCCAACTGTCCCACCGCCAGCAGAACGCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACAAAAAAGCCAGCTTACGCTGGCTTTTTcgt < 1:346311/126‑1 (MQ=39) tCCCACCGCCAGCAGAACGCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCTGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGTCTGACTGGCAGCAAGGCCATTTCCCt < 1:417820/150‑1 (MQ=255) tCCCACCGCCAGCAGAACGCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGTCTGACTGGCCGCAAGGCCATTTCCCt > 1:288708/1‑150 (MQ=255) ccACCGCCAGCAGAACGCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGTCTGACTGGCAGCAAGGCCATTTCCCTGc > 1:807573/1‑150 (MQ=255) ccACCGCCAGCAGAACGCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACAAAAAAGCCAGCTTACGCTGGCtttttc < 2:812084/114‑2 (MQ=39) ccACCGCCAGCAGAACGCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACAAAAAAGCCAGCTTACGCTGGCtttttc > 1:812084/1‑113 (MQ=39) aCCGCCAGCAGAACGCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGTCTGACTGGCAGCAAGGCCATTTCCCTGCgg > 1:212401/1‑150 (MQ=255) gcagAACGCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGTCTGACTGGCAGCAAGGCCATTTCCCTGCGGGATTGtt < 1:561931/150‑1 (MQ=255) cagAACGCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGTCTGACTGGCAGCAAGGCCATTTCCCTGCGGGATTGTTc < 2:807573/150‑1 (MQ=255) gCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGTCTGACTGGCAGCAAGGCCATTTCCCTGCGGGAtt > 2:331885/1‑140 (MQ=255) gCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGTCTGACTGGCAGCAAGGCCATTTCCCTGCGGGAtt < 1:331885/140‑1 (MQ=255) cTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGTCTGACTGGCAGCAAGGCCATTTCCCTGCGGGATTGTTCCCACAggg > 1:490919/1‑150 (MQ=255) gTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGTCTGACTg < 2:347687/107‑1 (MQ=255) gTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGTCTGACTg > 1:347687/1‑107 (MQ=255) cGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGTCTGACTGGCAGCAAGGCCATTTCCCTGCGGGATTGTTCCCACAGGGAGGAtt < 1:791445/150‑1 (MQ=255) gCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGTCTGACTGGCAGCAAGGCCATTTCCCTGCGGGATTGTTCCCACAGGGAGGATTGGCTTacaac < 1:505078/150‑1 (MQ=255) tCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGt > 2:353079/1‑86 (MQ=255) tCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGt < 1:353079/86‑1 (MQ=255) tgGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGTCTGACTGGCAGCAAGGCCATTTCCCTGCgg < 1:510640/108‑1 (MQ=255) tgGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGTCTGACTGGCAGCAAGGCCATTTCCCTGCgg > 2:510640/1‑108 (MQ=255) ggAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGTCTGACTGGCAGCAAGGCCATTTCCCTGCGGGATTGTTCCCACAGGGAGGATTGGCTTACAACAGGAACAGCGt < 2:454668/150‑1 (MQ=255) aaaTGGCCTCAGACGCCTGCTGAGGGCTGCAGCGAACGCGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGTCTGACTGGCAGCAAGGTCATTTCCCTGCGGCATTGTTCCCACAGGGAGGATTGGCTTACAACAGGTACAGCGTgg < 2:212401/150‑1 (MQ=255) cAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGTCTGACTGGCAGCAAGGCCATTTCCCTGCGGGATTGTTCCCACAGGGAGGATTGGCTTACAACAGGAACAGCGTGGCCAGCCCaa < 2:490919/150‑1 (MQ=255) cctcctGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGTCTGACTGGCAGCAAGGCCATTTCCCTGCGGGATTGTTCCCACAGGGAGGATTGGCTTACAACAGGAACAGCGTGGCCAGCCCAAGGAAGa > 1:13473/1‑150 (MQ=255) | AGCCCTGAACTGTCCCTGCATGAGCGCGAGTTGCTGCAGCGTTTCCGCCAACTGTCCCACCGCCAGCAGAACGCCTTGGTCGCCCTCATCGCTCACGATGTGGAAATGGCCGCAGACGCCTCCTGAGGGCTGCAGCGAACACGAAAAAGCCAGCGTAAGCTGGCTTTTTTGTGGGCGTCTGACTGGCAGCAAGGCCATTTCCCTGCGGGATTGTTCCCACAGGGAGGATTGGCTTACAACAGGAACAGCGTGGCCAGCCCAAGGAAGA > NC_002947/5077128‑5077395 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |