Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,131,909 T→G 11.5% L72R (CTG→CGG)  PP_4517 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,131,9090TG11.5% 101.9 / 6.5 43L72R (CTG→CGG) PP_4517hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (18/20);  new base G (2/3);  total (20/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

GCGCTGGACTATGTCGCCGCACACGGCTACCTCACCGCTCTTTCGATCAACGCCGAGGACGTGCCCGAGCGCGAATGGATCGACGCCCTGTTCGCCGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAATTCGACCTGCCCTGCGACCTCGACCTGACCGATGAGCCGGACGATTCCGACCTGCGCGGCTGGTGCATCGGCTTCATGGAAGGCGTGTTCCTGCGGGA  >  NC_002947/5131761‑5132056
                                                                                                                                                      |                                                                                                                                                     
gcgcTGGACTATGTCGCCGCACACGGCTACCTCACCGCTCTTTCGATCAACGCCGAGGACGTGCCCGAGCGCGAATGGATCGACGCCCTGTTCGCCGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCtg                                                                                                                                                      >  2:698061/1‑150 (MQ=255)
    tGGACTATGTCGCCGCACACGGCTACCTCACCGCTCTTTCGATCAACGCCGAGGACGTGCCCGAGCGCGAATGGATCGACGCCCTGTTCGCCGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCtggtgg                                                                                                                                                  >  1:845872/1‑150 (MQ=255)
       aCTATGTCGCCGCACACGGCTACCTCACCGCTCTTTCGATCAACGCCGAGGACGTGCCCGAGCGCGAATGGATCGACGCCCTGTTCGCCGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCAGGGTGgcct                                                                                                                                               <  2:82877/150‑3 (MQ=255)
        cTATGTCGCCGCACACGGCTACCTCACCGCTCTTTCGATCAACGCCGAGGACGTGCCCGAGCGCGAATGGATCGACGCCCTGTTCGCCGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCAGGGTGgcctc                                                                                                                                              <  2:408019/150‑4 (MQ=255)
         tATGTCGCCGCACACGGCTACCTCACCGCTCTTTCGATCAACGCCGAGGACGTGCCCGAGCGCGAATGGATCGACGCCCTGTTCGCCGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTc                                                                                                                                             <  1:263075/150‑1 (MQ=255)
            gTCGCCGCACACGGCTACCTCACCGCTCTTTCGATCAACGCCGAGGACGTGCCCGAGCGCGAATGGATCGACGCCCTGTTCGCCGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAg                                                                                                                                          >  1:528863/1‑150 (MQ=255)
            gTCGCCGCACACGGCTACCTCACCGCTCTTGCGATCAACGCCGAGGACGTGCCCGAGCGCGAATGGACCGCCGCCCGGTTCGCCGAGGATGGGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAg                                                                                                                                          <  1:104870/150‑1 (MQ=255)
               gccgcACACGGCTACCTCACCGCTCTTTCGATCAACGCCGAGGACGTGCCCGAGCGCGAATGGATCGACGCCCTGTTCGCCGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGca                                                                                                                                       <  1:129864/150‑1 (MQ=255)
                ccgcACACGGCTACCTCACCGCTCTTTCGATCAACGCCGAGGACGTGCCCGAGCGCGAATGGATCGACGCCCTGTTCGCCGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGcac                                                                                                                                      >  1:702464/1‑150 (MQ=255)
                    acacGGCTACCTCACCGCTCTTTCGATCAACGCCGAGGACGTGCCCGAGCGCGAATGGATCGACGCCCTGTTCGCCGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACAt                                                                                                                                  >  1:747763/1‑150 (MQ=255)
                      acGGCTACCTCACCGCTCTTTCGATCAACGCCTATTACGTGCCCGAGCGCGAATGGATCGACGCCCTGTTCGCCGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTg                                                                                                                                <  2:747763/150‑1 (MQ=255)
                        ggCTACCTCACCGCTCTTTCGATCAACGCCGAGGACGTGCCCGAGCGCGAATGGATCGACGCCCTGTTCGCCGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTGcc                                                                                                                              >  2:627796/1‑150 (MQ=255)
                              cTCACCGCTCTGTCGATCAACGCCGAGGACGTGCCCGAGCGCGAATGGATCGACTCCCTGTTCGCCGAGGAGCCGCCCTACGCCAGCGAAGCCCAGCGCACCGAGACCCAG‑GCCA‑CCCGGGCGGCGCTCAAGGCACACATTGCCCGcccc                                                                                                                        >  2:393539/1‑148 (MQ=255)
                                            gATCAACGCCGAGGACGTGCCCGAGCGCGAATGGATCGACGCCCTGTTCGCCGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGTCCCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGcgacga                                                                                                        >  1:649215/1‑150 (MQ=255)
                                                     cGAGGACGTGCCCGAGCGCGAATGGATCGACGCCCTGTTCGCCGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAATTCGa                                                                                               <  1:627796/150‑1 (MQ=255)
                                                      gAGGACGTGCCCGAGCGCGAATGGATCGACGCCCTGTTCGCCGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAATTCGAc                                                                                              <  1:382929/150‑1 (MQ=255)
                                                                        gAATGGATCGACGCCCTGTTCGCCGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAGCGCCA‑CCAGGGTGgcctcg                                                                                                                                             <  1:247455/88‑5 (MQ=255)
                                                                        gAATGGATCGACGCCCTGTTCGCCGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAGCGCCA‑CCAGGGTGgcctcg                                                                                                                                             >  2:247455/1‑84 (MQ=255)
                                                                          aTGGATCGACGCCCTGTTCGCCGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGAGGACGAATACCACCTGCCCTGcgagctccacct                                                                          >  2:130497/1‑150 (MQ=255)
                                                                          aTGGATCGACGCCCTGTTCGCCGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAATTCGACCTGCCCTGcgacctcgacct                                                                          <  2:845872/150‑1 (MQ=255)
                                                                             gATCGACGCCCTGTTCGCCGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGCTGGCGCACAAGGCACACGTTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAAGTCGACCTGCCCGTCGTCCTCGTGCTGAc                                                                       >  2:393849/1‑150 (MQ=255)
                                                                                   cGCACTGTTCGCCGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAATTCGACCTGCCCTGCGACCTCGACCTGACCGATGa                                                                 <  1:393539/150‑1 (MQ=255)
                                                                                         gTTCTCCGAGGTGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCTACGAAGAATTCGACCTGCCCTGCGACCTCGACCTGACCGATGAGCCGGa                                                           <  1:130497/150‑1 (MQ=255)
                                                                                             gCCGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAATTCGACCTGCCCTGCGACCTCGACCTGACCGATGAGCCGGa                                                           >  2:174207/1‑146 (MQ=255)
                                                                                             gCCGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAATTCGACCTGCCCTGCGACCTCGACCTGACCGATGAGCCGGa                                                           <  1:174207/146‑1 (MQ=255)
                                                                                               cGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAATTCGACCTGCCCTGCGACCTCGACCTGACCGATGAGCCGGACGATTc                                                     >  1:127422/1‑150 (MQ=255)
                                                                                                 aggagCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAATTCGACCTGCCCTGCGACCTCGACCTGACCGATGAGCCGGACGATTCCg                                                   <  1:393849/150‑1 (MQ=255)
                                                                                                   gagCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAATTCGACCTGCCCTGCGACCTCGACCTGACCGATGAGCCGGACGATTCCGAc                                                 <  2:528863/150‑1 (MQ=255)
                                                                                                                ccAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAATTCGACCTGCCCTGCGACCTCGACCTGACCGATGAGCCGGACGATTCCGACCTGCGCGGCTGGt                                    <  2:200451/150‑1 (MQ=255)
                                                                                                                   gCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAATTCGACCTGCCCTGCGACCTCGACCTGACCGATGAGCCGGACGAtt                                                      <  1:133371/129‑1 (MQ=255)
                                                                                                                   gCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAATTCGACCTGCCCTGCGACCTCGACCTGACCGATGAGCCGGACGAtt                                                      >  2:133371/1‑129 (MQ=255)
                                                                                                                     gAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACgaa                                                                                                       <  1:630715/78‑1 (MQ=255)
                                                                                                                     gAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACgaa                                                                                                       >  2:630715/1‑78 (MQ=255)
                                                                                                                     gAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAATTCGACCTGCCCTGCGACCTCGACCTGACCGATGAGCCGGACGATTCCGACCTGCGCGGCTGGTGCATc                               <  2:127422/150‑1 (MQ=255)
                                                                                                                     aaaGCCCAGCGCACCGCCATCG‑G‑GCCATGCGTGGTGGTGGGGAAGGCACACATTGCCCGCCAGCATTCGCCATC‑‑TTTAGAATTCGACCTGCCCTGCGACCTCGACCTGACCGATGAGCCGGACGATTCCGACCTGCGCGGCTGGTGCATc                               <  1:787255/149‑1 (MQ=255)
                                                                                                                           cAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAATTCGACCTGCCCTGCGACCTCGACCTGACCGATGAGCCGGACGATTCCGACCTGCGCGGCTGGTGCATCGGCTTc                         >  2:761073/1‑150 (MQ=255)
                                                                                                                                   cGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAATTCGACCTGCCCTGCGACCTCGACCTGACCGATGAGCCGGACGATTCCGACCTGCGCGGCTGGTGCATc                               <  2:250251/136‑1 (MQ=255)
                                                                                                                                   cGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAATTCGACCTGCCCTGCGACCTCGACCTGACCGATGAGCCGGACGATTCCGACCTGCGCGGCTGGTGCATc                               >  1:250251/1‑136 (MQ=255)
                                                                                                                                          gAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAATTCGACCTGCCCTGCGACCTCGACCTGACCGATGCGACCGTCTATTCCGACCTGCGCGGCTGGTGCATCGGCTTCATGGAAGGCGTGTTc          >  1:692332/1‑150 (MQ=255)
                                                                                                                                                 a‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAATTCGACCTGCCCTGCGACCTCGACCTGACCGATGAGCCGGACGATTCCGa                                                  >  1:806919/1‑104 (MQ=255)
                                                                                                                                                 a‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAATTCGACCTGCCCTGCGACCTCGACCTGACCGATGAGCCGGACGATTCCGa                                                  <  2:806919/104‑1 (MQ=255)
                                                                                                                                                   cccTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAATTCGACCTGCCCTGCGACCTCGACCTGACCGATGAGCCGGACGATTCCGACCTGCGCGGCTGGTGCATCGGCTTCATGGAAGGCGTGTTCCTGCggg   >  1:288065/1‑150 (MQ=255)
                                                                                                                                                    ccTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGACGAATTCGACCTGCCCTGCGACCTCGACCTGACCGATGAGCCGGACGATTCCGACCTGCGCGGCTGGTGCATCGGCTTCATGGAAGGCGTGTTCCTGCGGGa  >  1:548486/1‑150 (MQ=255)
                                                                                                                                                    ccTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAATTCGACCTGCCCTGCGACCTCGACCTGACCGATGAGCCGGACGATTCCg                                                   >  1:75253/1‑101 (MQ=255)
                                                                                                                                                    ccTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAATTCGACCTGCCCTGCGACCTCGACCTGACCGATGAGCCGGACGATTCCg                                                   <  2:75253/101‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GCGCTGGACTATGTCGCCGCACACGGCTACCTCACCGCTCTTTCGATCAACGCCGAGGACGTGCCCGAGCGCGAATGGATCGACGCCCTGTTCGCCGAGGAGCCGCACTACGCCAGCGAAGCCCAGCGCACCGAGATCGAG‑GCCA‑CCCTGGTGGCGCTCAAGGCACACATTGCCCGCCAGC‑‑TGGCCAGCGACGAAGAATTCGACCTGCCCTGCGACCTCGACCTGACCGATGAGCCGGACGATTCCGACCTGCGCGGCTGGTGCATCGGCTTCATGGAAGGCGTGTTCCTGCGGGA  >  NC_002947/5131761‑5132056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑