Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,152,176 G→A 13.4% intergenic (‑205/+1209) PP_5696 ← / ← PP_4537 hypothetical protein/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,152,1760GA13.4% 63.0 / 3.9 30intergenic (‑205/+1209)PP_5696/PP_4537hypothetical protein/membrane protein
Reads supporting (aligned to +/- strand):  ref base G (15/11);  new base A (2/2);  total (17/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.05e-01

TGATAAATGCCGTTCCGGAACTCGGGCATCGTGTGTTCAAGGCCGCAAAATGAATCGGCGGGCAAGCCATTGCGCATAAGGAAGAACTTCATCGACTTGGTGTTTAGCTCGGTCGTACAGGTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAGGTAGAGATCCACCATAGGCTTGAAAAGCACACCGTCGGTGTACAAATCGGAGATCGTTGTCTGGGCAACCACTCGATGCAGGGCCGCCCGCTTCAGTTGCTGCTCG  >  NC_002947/5152032‑5152325
                                                                                                                                                |                                                                                                                                                     
tgATAAATGCCGTTCCGGAACTCGGGCATCGTGTGTTCAAGGCCGCAAAATGAATCGGCGGGCAAGCCATTGCGCATAAGGAAGAACTTCATCGACTTGGTGTTTAGCTCGGTCGTACAGGTAACGCCAGTGCAGTCCAGCATGACCAGc                                                                                                                                                  >  1:482474/1‑150 (MQ=255)
           gTTCCGGAACTCGGGCATCGTGTGTTCAAGGCCGCAAAATGAATCGGCGGGCAAGCCATTGCGCATAAGGAAGAACTTCATCGACTTGGTGTTTAGCTCGGTCGTACAGGTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTa                                                                                                                                       <  1:24604/150‑1 (MQ=255)
                           aTCGTGTGTTCAAGGCCGCAAAATGAATCGGCGGGCAAGCCATTGCGCATAAGGAAGAACTTCATCGACTTGGTGTTTAGCTCGGTCGTACAGGTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAgg                                                                                                                       <  1:13898/150‑1 (MQ=255)
                           aTCGTGTGTTCAAGGCCGCAAAATGAATCGGCGGGCAAGCCATTGCGCATAAGGAAGAACTTCATCGACTTGGTGTTTAGCTCGGTCGTACAGGTAACGCCAGTGCAGTCCAGCATGACCAGCTCACGCCCGTACTGATGCTGGGTCAgg                                                                                                                       <  2:482474/150‑1 (MQ=255)
                                     cAAGTCCGCAAAATGAATCGGCGGGCAAGCCATTGCGCATAAGGAAGAACTTCATCGACTTGGTGTTTAGCTCGGTCGTACAGGTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGa                                                                                                             <  1:164933/150‑1 (MQ=255)
                                      aaGGCCGCAAAATGAATCGGCGGGCAAGCCATTGCGCATAAGGAAGAACTTCATCGACTTGGTGTTTAGCTCGGTCGTACAGGTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAg                                                                                                            >  1:107870/1‑150 (MQ=255)
                                      aaGGCCGCAAAATGAATCGGCGGGCAAGCCATTGCGCATAAGGAAGAACTTCATCGACTTGGTGTTTAGCTCGGTCGTACAGGTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAg                                                                                                            >  2:613393/1‑150 (MQ=255)
                                            gCAAAATGAATCGGCGGGCAAGCCATTGCGCATAAGGAAGAACTTCATCGACTTGGTGTTTAGCTCGGTCGTACAGGTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAGGTagag                                                                                                      >  1:2912/1‑150 (MQ=255)
                                                      tCGGCGGGCAAGCCATTGCGCATAAGGAAGAACTTCATCGACTTGGAATTTTGCTCGGTCGTACAGGTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAGGTAGAGATCCACCATa                                                                                            >  1:239421/1‑150 (MQ=255)
                                                           gggCAAGCCATTGCGCATAAGGAAGAACTTCATCGACTTGGTGTTTAGCTCGGTCGTACAGGTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAGGTGGAGATCCACCATAGGCtt                                                                                       >  2:228032/1‑150 (MQ=255)
                                                                     ttGCGCATAAGGAAGAACTTCATCGACTTGGTGTTTAGCTCGGTCGTACAGGTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAGGTAGCGATCCACCATAGACTTTAAAAAcaca                                                                             >  2:689147/1‑150 (MQ=255)
                                                                            tAAGGAAGAACTTCATCGACTTGGTGTTTAGCTCGGTCGTACAGGTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAGGTGGAGATCCACCATAGGCTTGAAAAGCACACCGTCgg                                                                      <  1:228032/150‑1 (MQ=255)
                                                                             aaGGAAGAACTTCATCGACTTGGTGTTTAGCTCGGTCGTACAGGTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAGGTAGAGATCCACCATAGGCTTGAAAAGCACACCGTCGgt                                                                     <  2:107870/150‑1 (MQ=255)
                                                                                       ttCATCGACTTGGTGTTTAGCTCGGTCGTACAGGTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAGGTAGAGATCCACCATAGGCTTGAAAAGCACACCGTCGGTGTACAAATCg                                                           >  2:440232/1‑150 (MQ=255)
                                                                                          aTCGACTTGGTGTTTAGCTCGGTCGTACAGGTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAGGTAGAGATCCACCATAGGCTTGAAAAGCACACCGTCGGTGTACAAATCGgag                                                        >  2:758799/1‑150 (MQ=255)
                                                                                                ttGGTGTTTAGCTCGGTCGTACAGGTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGa                                                                                                             <  1:374670/91‑1 (MQ=255)
                                                                                                ttGGTGTTTAGCTCGGTCGTACAGGTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGa                                                                                                             >  2:374670/1‑91 (MQ=255)
                                                                                                  ggTGTTTAGCTCGGTCGTACAGGTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAGGTAGAGATCCACCATAGGCTTGAAAAGCACACCGTCGGTGTACAAATCGGAGATCGTTGt                                                >  2:321701/1‑150 (MQ=255)
                                                                                                      tttAGCTCGGTCGTACAGGTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAGGTAGAGATCCACCATAGGCTTGAAAAGCACACCGTCGGTGTACAAATCGGAGATCGTTGTCTgg                                            <  1:321701/150‑1 (MQ=255)
                                                                                                               gTCGTACAGGTAACGCCAGTGCAGTCCAGCATGGCCAGCTCAAGGCCGTACTGATGCTGGGTCAGGTGATTGCTGAGGTAGAGATCCACCATAGGCTTGAAAAGCACACCGTCGGTGTACAAATCGGAGATCGTTGTCTGGGCAACCACt                                   >  1:835898/1‑150 (MQ=255)
                                                                                                                tCGTACAGGTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAGGTAGAGATCCACCATAGGCTTGAAAAGCACACCGTCGGTGTACAAATCGGAGATCGTTGTCTGGGCAACCACTc                                  >  2:588981/1‑150 (MQ=255)
                                                                                                                  gTACAGGTAACGCCAGTGCAGCCCACTGTGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAGGTAGAGATCCACCATAGGCTTGAAAAGCACACCGTCGGTGTACAAATCGGAGATCGTTGTCTGGGCAACCACTCGa                                <  2:835898/150‑1 (MQ=255)
                                                                                                                   tACAGGTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAGGTAGAGATCCACCATAGGCTTGAAAAGCACACCGTCGGTGTACAAATCGGAGATCGTTGTCTGGGCAACCACTCGAt                               <  2:2912/150‑1 (MQ=255)
                                                                                                                       ggTAACGCCAGTGCAGTCCAGCATGACCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAGGTAGAGATCCACCATAGGCTTGAAAAGCACACCGACGgtgtgctt                                                               >  2:541936/1‑110 (MQ=255)
                                                                                                                       ggTAACGCCAGTGCAGTCCAGCATGACCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAGGTAGAGATCCACCATAGGCTTGAAAAGCACACCGACGgtgtgctt                                                               <  1:541936/114‑5 (MQ=255)
                                                                                                                        gTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAGGTAGAGATCCACCATAGGCTTGAAAAGca                                                                               >  2:478460/1‑97 (MQ=255)
                                                                                                                        gTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAGGTAGAGATCCACCATAGGCTTGAAAAGca                                                                               <  1:478460/97‑1 (MQ=255)
                                                                                                                        gTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAGGTAGAGATCCACCATAGGCTTGAAAAGCACACCGTCGGTGTACAAATCGGAGATCGTTGTCTGGGCAACCACTCGATGCAgg                          <  1:758799/150‑1 (MQ=255)
                                                                                                                        gTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAGGTAGAGATCCACCATAGGCTTGAAAAGCACACCGTCGGTGTACAAATCGGAGATCGTTGTCTGGGCAACAACTCGATGCAgg                          >  1:387342/1‑150 (MQ=255)
                                                                                                                                           gCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAGGTAGAGATCCACCATAGGCTTGAAAAGCACACCGTCGGTGTACAAATCGGAGATCGTTGTCTGGGCAACCACTCGATGCAGGGCCGCCCGCTTCAGTtgct       >  2:302594/1‑150 (MQ=255)
                                                                                                                                                gCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAGGTAGAGATCCACCATAGGCTTGAAAAGCACACCGTCGGTGTACAAATCGGAGATCGTTGTCTGGGCAACCACTCGATGCAGGGCCGCCCGCTTCAGTTGCTGCTcg  >  1:769535/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
TGATAAATGCCGTTCCGGAACTCGGGCATCGTGTGTTCAAGGCCGCAAAATGAATCGGCGGGCAAGCCATTGCGCATAAGGAAGAACTTCATCGACTTGGTGTTTAGCTCGGTCGTACAGGTAACGCCAGTGCAGTCCAGCATGGCCAGCTCACGCCCGTACTGATGCTGGGTCAGGTGATTGCTGAGGTAGAGATCCACCATAGGCTTGAAAAGCACACCGTCGGTGTACAAATCGGAGATCGTTGTCTGGGCAACCACTCGATGCAGGGCCGCCCGCTTCAGTTGCTGCTCG  >  NC_002947/5152032‑5152325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑