Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,159,939 C→A 9.3% K388N (AAG→AAT)  PP_4543 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,159,9390CA 9.3% 121.5 / 2.3 43K388N (AAG→AAT) PP_4543hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (18/21);  new base A (2/2);  total (20/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

GCGGCCTTTTCGCGGGTAAACCCGCTCCTACACAGGTTCGCTATCGT‑TGTAGGAGCGGGTTTACCCGCGAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGCAGCAGGTAATGCCACGACCGCGTCAACACCGGCTCGAACCCGCGTATCAACTTGTGCTCGCCCTGTGCCCCGGCATCGAAACGCTGG  >  NC_002947/5159793‑5160086
                                                                                                                                                   |                                                                                                                                                   
gcgGCCTTTTCGCGGGTAAACCCGCTCCTACACAGGTTCGCTATCGT‑TGTAGGAGCGGGTTTACCCGCGAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCATTg                                                                                                                                                  >  1:624795/1‑150 (MQ=255)
gcgGCCTTTTCGCGGGTAAACCCGCTCCTACACAGGATCGCTATCGT‑TGTAGGAGCGGGTTTACCCGCGAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCATTg                                                                                                                                                  >  1:623897/1‑150 (MQ=255)
 cgGGCTTTTCGCGGGTAAACCCGCTCCTACAAAGGTTCGCGATCGT‑TGTAGGAGCGGGTTTACCCGCGAAAAGGCCGGCACAGGCAACACAGCTCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGa                                                                                                                                                 <  2:173762/150‑1 (MQ=255)
  ggCCTTTTCGCGGGTAAACCCGCTCCTACA‑ACGATAGCGAACCTGTGTAGGAGCGGGTTTACCCGCGAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGAc                                                                                                                                                >  2:435372/1‑150 (MQ=18)
    ccTTTTCGCGGGTAAACCCGCTCCTACACAGGTTCGCTATCGT‑TGTAGGAGCGGGTTTACCCGAGAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCACTCCCCTTCCCCCTACCCCTTTActc                                                                                                                                              >  2:378393/1‑148 (MQ=37)
                    cccGCTCCTACACAGGTTCGCTATCGT‑TGTAGGAGCGGGTTTACCCGCGAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTgcag                                                                                                                              >  2:718731/1‑150 (MQ=255)
                    cccGCTCCTACACAGGTTCGCTATCGT‑TGTAGGAGCGGGTTTACCCGCGAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTgcag                                                                                                                              >  2:66914/1‑150 (MQ=255)
                     ccGCTCCTACACAGGTTCGCTATCGT‑TGTAGGAGCGGGTTTACCCGCGAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTCGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTgcagc                                                                                                                             >  2:344411/1‑150 (MQ=255)
                               acaGGTTCGCTATCGT‑TGTAGGAGCGGGTTTACCCGCGAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAAtcct                                                                                                                   >  2:174673/1‑150 (MQ=255)
                                cacgtgcGCTATCGT‑TGTACGAGCGAGTTTCCCCGCGAAAAGGACGGCACAGGCAACTCAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAAtcctc                                                                                                                  <  1:698036/144‑1 (MQ=255)
                                caGGTTCGCTATCGT‑TGTAGGAGCGGGTTTACCCGCGAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAAtcctc                                                                                                                  <  1:88349/150‑1 (MQ=255)
                                 aGGTTCGCTATCGT‑TGTAGGAGCGGGTTTACCCGCGAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAAtcctcc                                                                                                                 >  2:794769/1‑150 (MQ=255)
                                                 gTAGGAGCGGGTTTACCCGCGAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAAc                                                                                                  >  2:126839/1‑150 (MQ=255)
                                                  tAGGATCGGGGTTACCCGCGAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAAcc                                                                                                 >  2:229409/1‑150 (MQ=255)
                                                    ggAGCGGGTTTACCCGCGAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAAcccc                                                                                               >  1:551473/1‑150 (MQ=255)
                                                     gAGCGGGTTTACCCGCGAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCg                                                                                              <  1:229409/150‑1 (MQ=255)
                                                      aGCGGGTTTACCCGCGAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCATTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCgg                                                                                             <  2:623897/150‑1 (MQ=255)
                                                      aGCGGGTTTACCCGCGAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCATTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCgg                                                                                             <  2:624795/150‑1 (MQ=255)
                                                       gCGGGTTTACCCGCGAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCggg                                                                                            <  1:668832/150‑1 (MQ=255)
                                                             gtACCCGCGAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTgcagc                                                                                      <  2:308123/149‑1 (MQ=255)
                                                              tACCCGCGAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTgcagca                                                                                     <  1:749588/150‑1 (MQ=255)
                                                               aCCCGCGAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGcagcag                                                                                    <  2:551473/150‑1 (MQ=255)
                                                                cccGCGAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGCAGTAgg                                                                                   <  1:794769/150‑1 (MQ=255)
                                                                 ccGCGAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGCAGCAGGt                                                                                  >  2:681441/1‑150 (MQ=255)
                                                                  cgcgAAAATGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGCAAATCCTCCACCGCCCGCCGCAACACCGGGTGACGCAGGTa                                                                                 >  2:263416/1‑150 (MQ=255)
                                                                     gAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGCAGCAGGTAATg                                                                              <  2:469504/150‑1 (MQ=255)
                                                                       aaaGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGCAGCAGGTAATGcc                                                                            <  1:435372/150‑1 (MQ=255)
                                                                        aaGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGCAGCAGGTAATGCCa                                                                           <  1:681441/150‑1 (MQ=255)
                                                                               gCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGCAGCAGGTAATGCCACGACcgc                                                                    <  1:378393/150‑1 (MQ=255)
                                                                                                ctctcAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGCAGCAGGTAATGCCACGACCGCGTCAACACCGGCTCGaa                                                   <  1:354664/150‑1 (MQ=255)
                                                                                                 tctcAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGCAGCAGGTAATGCCACGACCGCGTCAACACCGGCTCGAAc                                                  <  1:718731/150‑1 (MQ=255)
                                                                                                     aGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGcagcag                                                                                    >  1:136237/1‑112 (MQ=255)
                                                                                                     aGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGcagcag                                                                                    <  2:136237/112‑1 (MQ=255)
                                                                                                            gcggTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGCAGCAGGTAATGCCACGACCGCGTCAACACCGGCTCGAACCCGCGTATCaa                                       <  2:2005/150‑1 (MQ=255)
                                                                                                                      aaCATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGCAGCAGGTAATGCCACGACCGCGTCAACACCGGCTCGAACCCGCGTATCAACTTGTGCTCg                             >  2:137915/1‑150 (MQ=255)
                                                                                                                          tCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGCAGCAGGTAATGCCACGACCGCGTCAACACCGGCTCGAACCCGCGTATCAACTTGTGCTCGCCCt                         <  1:344411/150‑1 (MQ=255)
                                                                                                                                     tctTCGGCATACCCCGTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGCAGCAGGTAATGCCACGACCGCGTCAACTCCGGCTCGAACCCGCGTATCAACTTGTGCTCGCCCTGTGCCCCGGCa              <  1:66914/150‑1 (MQ=255)
                                                                                                                                       ttCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGCAGCAGGTAATGCCACGACCGCGTCAACACCGGCTCGAACCCGCGTATCAACTTGTGCTCGCCCTGTGCCCCGGCATc            >  1:704490/1‑150 (MQ=255)
                                                                                                                                          ggCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGCAGCAGGTAATGCCACGACCGCGTCAACACCGGCTCGAAcc                                                 >  1:418428/1‑110 (MQ=255)
                                                                                                                                          ggCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGCAGCAGGTAATGCCACGACCGCGTCAACACCGGCTCGAAcc                                                 <  2:418428/110‑1 (MQ=255)
                                                                                                                                          ggCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGCAGCAGGTAATGCCACGACCGCGTCAACACCGGCTCGAACCCGCGTATCAACTTGTGCTCGCCCTGTGCCCCGGCATCGaa         >  1:80469/1‑150 (MQ=255)
                                                                                                                                              tACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCCCCCGCCTCATCCCCGGGTGCAGCAGGTCATGCCACGACCGCGTCAACACCGGCTCGAACCCGCGTATCAACTTGTGCTCGCCCTGTGCCCCGGCATCGAAACGc     <  1:697206/150‑1 (MQ=255)
                                                                                                                                               aCCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCTTCCACCGGTTGCTGCAAGCCCTTGTGCATCAGGAATTGCCACGACCGCGTCAACACCGGCGCGAACCCGCGTATCAACTTGTGCTCGCCCTGTGCCCCGGCATCGAAACGCt    >  1:233769/1‑150 (MQ=255)
                                                                                                                                                ccccTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGCAGCAGGTAATGCCACGACCGCGTCAACACCGGCTCGAACCCGCGTATCAACTTGTGCTCGCCCTGTGCCCCGGCATCGAAACGCTg   <  1:174673/150‑1 (MQ=255)
                                                                                                                                                 cccTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGCAGCAGGTAATGCCACGACCGCGTCAACACCGGCTCGAACCCGCGTATCAACTTGTGCTCGCCCTGTGCCCCGGCATCGAAACGCTgg  >  2:451755/1‑150 (MQ=255)
                                                                                                                                                  ccTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGCAGCAGGTAATGCCACGACCGCGTCAACACCGGCTCGAACCCGCGTATCAAc                                      <  2:610532/113‑1 (MQ=255)
                                                                                                                                                  ccTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGCAGCAGGTAATGCCACGACCGCGTCAACACCGGCTCGAACCCGCGTATCAAc                                      >  1:610532/1‑113 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCGGCCTTTTCGCGGGTAAACCCGCTCCTACACAGGTTCGCTATCGT‑TGTAGGAGCGGGTTTACCCGCGAAAAGGCCGGCACAGGCAACACAGATCTCTCAGTCCCGGCGGTAGGGCAACATCCCCCGCGCCTCTTCGGCATACCCCTTGACCCCTTCCCGTTCCTGCAGCAGAAAATCCTCCACCGCCCGCCGCAACCCCGGGTGCAGCAGGTAATGCCACGACCGCGTCAACACCGGCTCGAACCCGCGTATCAACTTGTGCTCGCCCTGTGCCCCGGCATCGAAACGCTGG  >  NC_002947/5159793‑5160086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑