Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,162,628	T→A	8.8%	intergenic (+103/+98)	PP_4544 → / ← PP_4545	hypothetical protein/beta‑ketodecanoyl‑ACP synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,162,628	0	T	A	8.8%	162.7 / 4.9	56	intergenic (+103/+98)	PP_4544/PP_4545	hypothetical protein/beta‑ketodecanoyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base T (28/23);  new base A (2/3);  total (30/26)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

CTGACGTCGACACCCTGCAGGTAGACCTGGAAGCCAAGTTCTAAGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAG  >  NC_002947/5162482‑5162777                                                                                                                                                   |                                                                                                                                                       cTGACGTCGACACCCTGCAGGTAGACCTGGAAGCCAAGTTCTAAGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGc                                                                                                                                                     <  1:311210/150‑1 (MQ=255)        cGACACCCTGCAGGTAGACCTGGAAGCCAAGTTCTAAGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCAAGCCCGcgaa                                                                                                                                              <  1:377552/150‑4 (MQ=255)         gACACCCTGCAGGTAGACCTGGAAGCCAAGTTCTAAGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCAAGCCCGcgaag                                                                                                                                             <  1:548389/150‑5 (MQ=255)          cttccacctgtaccaggatgacctggccaaaggctcgttgggcgtgttgagctcgtttggtgcggggtattcgattggtagtgtgattctgcgcaagcgctgattcatcaggcgcctgttacggcctCTTCGCGGGCTTGCCCGCTCCca                                                                                                                                            <  1:729876‑M1/23‑1 (MQ=255)              ccTGCAGGTAGACCTGGAAGCCAAGTTCTAAGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCca                                                                                                                                            <  2:218758/146‑1 (MQ=255)              ccTGCAGGTAGACCTGGAAGCCAAGTTCTAAGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCca                                                                                                                                            >  1:218758/1‑146 (MQ=255)               cTGCAGGTAGACCTGGAAGCCAAGTTCTAAGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCAAGCCCGcgaagg                                                                                                                                            >  2:244187/1‑140 (MQ=255)               cTGCAGGTAGACCTGGAAGCCAAGTTCTAAGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCAAGCCCGcgaagg                                                                                                                                            <  1:244187/145‑6 (MQ=255)                     gTAGACCTGGAAGCCAAGTTCTAAGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATcacca                                                                                                                                <  2:573433/150‑1 (MQ=255)                           cTGGAAGCCAAGTTCTAAGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGc                                                                                                                                                     <  1:618346/124‑1 (MQ=255)                           cTGGAAGCCAAGTTCTAAGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGc                                                                                                                                                     >  2:618346/1‑124 (MQ=255)                                  ggccaaaggctcgttgggcgtgttgagctcgtttggtgcggggtattcgattggtagtgtgattctgcgcaagcgctgattcatcaggcgcctgttacggcctCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGaa                                                                                                                   >  1:63108‑M1/104‑150 (MQ=255)                                   cAAGTTCTAAGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAAc                                                                                                                  >  1:287266/1‑150 (MQ=255)                                       ttCTAAGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACtgtg                                                                                                              >  2:105980/1‑150 (MQ=255)                                         cTAAGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCTACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCt                                                                                                            <  1:815542/150‑1 (MQ=255)                                          gctcgttgggcgtgttgagctcgtttggtgcggggtattcgattggtagtgtgattctgcgcaagcgctgattcatcaggcgcctgttacggcctCTTCGCGGGCTTGCCCGCTCCCACAGGTA‑CACCACAGACCTTGAGAACTGTGCTg                                                                                                           >  1:222191‑M1/96‑150 (MQ=255)                                                  acCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATc                                                                                                                                    >  2:721569/1‑117 (MQ=255)                                                  acCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATc                                                                                                                                    <  1:721569/117‑1 (MQ=255)                                                   gcgtgttgagctcgtttggtgcggggtattcgattggtagtgtgattctgcgcaagcgctgattcatcaggcgcctgttacggcctCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTgg                                                                                                  >  1:251963‑M1/87‑150 (MQ=255)                                                   gcgtgttgagctcgtttggtgcggggtattcgattggtagtgtgattctgcgcaagcgctgattcatcaggcgcctgttacggcctCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTgg                                                                                                  >  1:394766‑M1/87‑150 (MQ=255)                                                    ccTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGc                                                                                                             >  2:20103/1‑138 (MQ=255)                                                    ccTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGc                                                                                                             <  1:20103/138‑1 (MQ=255)                                                    ccTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTggg                                                                                                 >  2:684772/1‑150 (MQ=255)                                                         tctgctTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCgg                                                                                            <  2:352648/150‑1 (MQ=255)                                                             ctTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCaa                                                                                        <  1:299577/150‑1 (MQ=255)                                                              ttCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGTGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGGGCTGTACCTGTGGGAGCGGGCAAg                                                                                       >  2:246462/1‑150 (MQ=255)                                                              ttCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCgg                                                                                            <  2:12073/145‑1 (MQ=255)                                                              ttCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCgg                                                                                            >  1:12073/1‑145 (MQ=255)                                                               tCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGcttgc                                                                                      <  1:105980/150‑5 (MQ=255)                                                                cACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGCGAGAGGGCAAGcc                                                                                     >  2:687643/1‑150 (MQ=255)                                                                  ccTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCAGAG‑TAACACCACAGACCTTGAAACGAGTGCTCTGCCCGTGGGAGCGGGCGAGCCcg                                                                                   >  1:645022/1‑150 (MQ=38)                                                                       aGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGAGCAAGCCCGCGAag                                                                              <  2:287266/150‑1 (MQ=255)                                                                         cGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAagag                                                                            >  2:747797/1‑150 (MQ=255)                                                                           ggAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGc                                                                          >  2:518636/1‑150 (MQ=255)                                                                           ggAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTCTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGc                                                                          >  1:114837/1‑150 (MQ=255)                                                                            gAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGCACTGTATTGGTGCTTTGGGAGCGGGCAAGCCCGCGAAGAGGcc                                                                         >  2:203210/1‑150 (MQ=38)                                                                                 gCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTCTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAAc                                                                    <  2:114837/150‑1 (MQ=255)                                                                                  ccGCCCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACa                                                                   <  1:340271/150‑1 (MQ=255)                                                                                        ccATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTgg                                                                                                  <  1:562164/113‑1 (MQ=255)                                                                                        ccATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTgg                                                                                                  >  2:562164/1‑113 (MQ=255)                                                                                           tCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCtga                                                          <  1:518636/150‑1 (MQ=255)                                                                                            tcGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCtgat                                                         <  1:257353/149‑1 (MQ=255)                                                                                               attctgcgcaagcgctgattcatcaggcgcctgttacggcctCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCgg                                                                                            >  2:826868‑M1/43‑112 (MQ=255)                                                                                               attctgcgcaagcgctgattcatcaggcgcctgttacggcctCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCgg                                                                                            <  1:826868‑M1/70‑1 (MQ=255)                                                                                                ttctgcgcaagcgctgattcatcaggcgcctgttacggcctCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGc                                                                                              <  1:536404‑M1/68‑1 (MQ=255)                                                                                                ttctgcgcaagcgctgattcatcaggcgcctgttacggcctCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGc                                                                                              <  1:411602‑M1/68‑1 (MQ=255)                                                                                                ttctgcgcaagcgctgattcatcaggcgcctgttacggcctCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGc                                                                                              >  2:536404‑M1/42‑109 (MQ=255)                                                                                                ttctgcgcaagcgctgattcatcaggcgcctgttacggcctCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGc                                                                                              >  2:411602‑M1/42‑109 (MQ=255)                                                                                                         cttcttTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGa                                                                                >  2:118803/1‑114 (MQ=255)                                                                                                         cttcttTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACGGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGa                                                                                <  1:118803/114‑1 (MQ=255)                                                                                                          ttcttTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCATGGCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTgc                                           >  1:623252/1‑150 (MQ=255)                                                                                                                   tCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATca                                  >  1:720536/1‑150 (MQ=255)                                                                                                                          gAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTAcc                           >  1:219529/1‑150 (MQ=255)                                                                                                                                          TTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCa           >  2:514411‑M1/1‑150 (MQ=255)                                                                                                                                             GCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGGACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGGGCAGAATCACACTCCCAATCGCATACCCCGCACCa        <  2:173129‑M1/150‑1 (MQ=255)                                                                                                                                                   TTGCCCGCTCCCACAC‑TAACAACAATGACCTTTAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAg  >  2:623252‑M1/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                       CTGACGTCGACACCCTGCAGGTAGACCTGGAAGCCAAGTTCTAAGCAACACCCTGCTCTGCTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAG‑TATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAG  >  NC_002947/5162482‑5162777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑