| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 413,107 | C→G | 11.0% | A686A (GCC→GCG) | glnE → | glutamate‑ammonia‑ligase adenylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 413,107 | 0 | C | G | 11.0% | 95.8 / 2.4 | 34 | A686A (GCC→GCG) | glnE | glutamate‑ammonia‑ligase adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (15/15); new base G (2/2); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCATTTCAAGCTGGCCCACAGCCTGCGCGTGGCCGCCTCGGAAATCAGCGGCAACCTGCCGTTGATGAAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGGACCTGGACCTGGTCTTCATCCATGACGGCGATCCGCAGGCGGAAAC > NC_002947/412959‑413256 | gcCATTTCAAGCTGGCCCACAGCCTGCGCGTGGCCGCAACGTAAATCAGCGGTAACCTGCAGTTGATGAAAGTCAGCGTCTACCCGACCGCGCCGTCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGGCCGTGGccc > 1:408842/1‑150 (MQ=255) ttCAAGCTGGCCCACAGCCTGCGCGTGGCCGCCTCGGAAATCAGCGGCAACCTGCCGTTGATGAAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCAc < 1:515126/150‑1 (MQ=255) aaGCTGGCCCACAGCCTGCGCGTGGCCGCCTCGGAAATCAGCGGCAACCTGCCGTTGATGAAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGc < 2:31104/150‑1 (MQ=255) aGCTGGCCCACAGCCTGCGCGTGGCCGCCTCGGAAATCAGCGGCAACCTGCCGTTGATGAAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCGGGCCACggtc < 2:762332/150‑3 (MQ=255) gCTGGCCCACAGCCTGCGCGTGGCCGCCTCGGAAATCAGCGGCAACCTGCCGTTGATGAAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCGGGCCACggtct < 1:335529/150‑4 (MQ=255) cTGGCCCACAGCCTGCGCGTCGCCGCCTCGGAAATCAGCGGCAACCTGCCGTTGATGAAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCaa < 1:838096/150‑1 (MQ=255) cTGCGCGTGGCCGCCTCGGAAATCAGCGGCAACCTGCCGTTGATGAAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGc < 2:565772/150‑1 (MQ=255) ccTCGGAAATCAGCGGCAACCTGCCGTTGATGAAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCtgt > 2:505350/1‑150 (MQ=255) ggCAACCTGCCGTTGATGAAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTGtc > 2:175186/1‑150 (MQ=255) ccGTTGATGAAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATc < 1:5607/150‑1 (MQ=255) cGTTGATGAAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCg < 1:6072/150‑1 (MQ=255) atgaAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTAc > 2:575083/1‑150 (MQ=255) gaAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTTGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACgg > 2:504033/1‑150 (MQ=255) cctgacctgGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACgg > 2:126039/1‑135 (MQ=255) cctgacctgGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACgg < 1:126039/135‑1 (MQ=255) ggctggctGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTTGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCg < 1:266547/150‑1 (MQ=255) gCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCg > 1:339395/1‑150 (MQ=255) aCCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACGCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCggacctggacc > 1:497186/1‑150 (MQ=255) ccAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCggacctggacct > 2:769910/1‑150 (MQ=255) aGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGGacctggacctgg < 1:81496/150‑1 (MQ=255) ggTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGGACCTGGACCTGGt > 1:839031/1‑150 (MQ=255) ggTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGGACCTGGACCTGGt < 1:645193/150‑1 (MQ=255) tggccctggccTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGTGCGTCCTGGAGCGCGGCCACGGTTCGGACCTGGACCTGGTCTTCa < 1:575083/150‑1 (MQ=255) gccctggccTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGGACCTGGACCTGGTCTTCATc > 2:410839/1‑150 (MQ=255) cctggcctggCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATggg > 2:663811/1‑102 (MQ=255) cctggcctggCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATggg < 1:663811/102‑1 (MQ=255) gaggcctggcGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGGACCTGGACCTGGTCTTCATCCAt > 2:549464/3‑150 (MQ=255) ctggcctggcGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCCGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGGACCTGGACCTGGTCTTCATCCAt < 1:175186/150‑1 (MQ=255) gcctggcGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGGACCTGGACCTGGTCTTCATCCATGAc < 2:408842/150‑1 (MQ=255) gcCAGACCGTGGCGCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGGACCTGGACCTGGTCTTCATCCATGACGGCGATc > 2:527090/1‑150 (MQ=255) gcCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGGACCTGGACCTGGTCTTCATCCATGACGGCGATc > 1:635532/1‑150 (MQ=255) gcCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGGACCTGGACCTGGTCTTCATCCATGACGGCGATc < 1:505350/150‑1 (MQ=255) ttggccGTGGCGGGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTCCGGGGAGCTGGGCGGCCTGGAGCTCGGCCACGGTTCGGACCTGGACCTGGTCTTCATCCATGACGGCGATCCg > 1:148766/5‑150 (MQ=255) aGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGGACCTGGACCTGGTCTTCATCCATGACGGCGATCCGc > 1:102567/1‑150 (MQ=255) cGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGGACCTGGACCTGGTCTTCATCCATGCCGGCGATCCGCAGGc > 2:146861/1‑150 (MQ=255) cccGCCACGGCGAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGCTGGGCGGCCTGGAGCTCGGCGACGGTACGGACCTGGACCTGGTCTTCATCCATGACGGCGATCCGCAGGCGGaaa < 2:379383/150‑1 (MQ=255) ccGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGGACCTGGACCTGGTCTTCATCCATGACGGCGATCCGCAGGCGGAAAc < 1:758378/150‑1 (MQ=255) | GCCATTTCAAGCTGGCCCACAGCCTGCGCGTGGCCGCCTCGGAAATCAGCGGCAACCTGCCGTTGATGAAAGTCAGCGACTACCTGACCTGGCTGGCTGAAGCCATCCTCGACCAGGTGCTGGCCCTGGCCTGGCGCCAGACCGTGGCCCGCCACGGCCAACCCAAGCGCAGCGACGGCAGCCTGTGCGACCCGGGTTTCATCATTATCGGTTACGGCAAGATGGGCGGCCTGGAGCTCGGCCACGGTTCGGACCTGGACCTGGTCTTCATCCATGACGGCGATCCGCAGGCGGAAAC > NC_002947/412959‑413256 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |