| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,254,096 | G→A | 10.2% | L196L (CTG→TTG) | PP_4630 ← | MerR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,254,096 | 0 | G | A | 10.2% | 103.8 / 3.6 | 39 | L196L (CTG→TTG) | PP_4630 | MerR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (18/17); new base A (2/2); total (20/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01 | |||||||||||
TGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGTCATAGAACAGCCATTCGCTGGCCTGCCCATAGCCGCCT > NC_002947/5253953‑5254244 | tgctgTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTc > 2:403085/1‑150 (MQ=255) tgGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCt > 1:210124/1‑150 (MQ=255) gCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCAt > 1:511741/1‑150 (MQ=255) gACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATgg < 2:541494/150‑1 (MQ=255) tGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCAc < 1:166561/150‑1 (MQ=255) gATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACt < 2:511741/150‑1 (MQ=255) gggAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGcc > 2:175485/1‑150 (MQ=255) ggAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGccc > 2:82528/1‑150 (MQ=255) gCACCAGGTCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCAGGCaaaa < 2:519856/150‑3 (MQ=255) gggCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCa > 1:846572/1‑125 (MQ=255) gggCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCa < 2:846572/125‑1 (MQ=255) cGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCAc < 1:403085/150‑1 (MQ=255) ggCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATg < 1:54520/119‑1 (MQ=255) ggCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATg > 2:54520/1‑119 (MQ=255) gCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCaa > 2:782646/1‑111 (MQ=255) gCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCaa < 1:782646/111‑1 (MQ=255) gCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACgg > 2:387807/1‑150 (MQ=255) cGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTa < 1:82528/150‑1 (MQ=255) aCCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGcaca < 1:387807/150‑1 (MQ=255) cAAACTACTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCa > 2:11108/1‑150 (MQ=255) aGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCgg < 2:210124/150‑1 (MQ=255) gCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAACAACTCAAGCTCa > 1:242097/1‑80 (MQ=255) gCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAACAACTCAAGCTCa < 2:242097/80‑1 (MQ=255) gCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAACAACTCAAGCTCa < 2:242194/80‑1 (MQ=255) gCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAACAACTCAAGCTCa > 1:242194/1‑80 (MQ=255) tGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAg > 1:625194/1‑62 (MQ=255) tGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAg < 2:625194/62‑1 (MQ=255) ttCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGa < 1:294664/150‑1 (MQ=255) ccAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACcgc > 1:772040/1‑150 (MQ=255) cAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCg > 2:449214/1‑77 (MQ=255) cAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCg < 1:449214/77‑1 (MQ=255) aTCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAAc > 2:731164/1‑150 (MQ=255) gtcgCTGCCCAGGATCAGGCTGGTTACCAGCAACTCCAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGTCATAGAACAg < 2:522960/150‑1 (MQ=255) gCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGTCATAGAACAGCCa > 1:697702/1‑150 (MQ=255) cccAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGTCATAGAACAGCCATTCg < 1:532418/150‑1 (MQ=255) cccAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGTCATAGAACAGCCATTCg > 2:532418/1‑150 (MQ=255) gCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCaa > 2:177541/1‑90 (MQ=255) gCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCaa < 1:177541/90‑1 (MQ=255) ggTTACCAGCAACTCAAGCTCAGGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGAGGGCGAAAACCCGACCGCGCAGGAACTGGTCATAGAACAGCCATTCGCTGGCCTGCCCATa < 2:317121/150‑1 (MQ=255) aGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGAGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGTCATAGAACAGCCATTCGCTGGCCTGCCCATAGCCGCCt > 1:278379/1‑150 (MQ=255) aGCAACTCAAGCTCATGACACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGTCATAGAACAGCCATTCGCTGGCCTGCCCATAGCCGCCt > 1:793150/1‑150 (MQ=255) | TGCTGTGGGCGACTGATGGGAAAACAGCACCAGGGCGTCCGGCTTCAGCCGTTCGCAGACCAGCGCAAGCTCCTGCAGCGGCTGCCCGTTCGCCAGCGGGATCACGCGTGTTTCGTCGCTGCCCAGGATCAGGCTGGTTACCAGCAACTCAAGCTCATGGCACTGCCCCGGCAATGGCGCCAATAGCACGGTGCGGCTACACGGCACACGGGCCAGCTGCAAGCGGGCGAAAACCCGACCGCGCAGGAACTGGTCATAGAACAGCCATTCGCTGGCCTGCCCATAGCCGCCT > NC_002947/5253953‑5254244 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |