| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,276,067 | C→T | 15.4% | M226I (ATG→ATA) | cioA ← | cyanide insensitive ubiquinol oxidase subunit I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,276,067 | 0 | C | T | 15.4% | 52.7 / 4.3 | 26 | M226I (ATG→ATA) | cioA | cyanide insensitive ubiquinol oxidase subunit I |
| Reads supporting (aligned to +/- strand): ref base C (13/9); new base T (2/2); total (15/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-01 | |||||||||||
GAACAGGATCAGTGGAGTCGGCTCACCGGGCACGTTCTCCCAGTGGCCTTCGATCGCAGCAATTTTCACCGGCTGGTGCTTGAGGGTATTGAGGCCGTGGAAATCGCCGATCACTGCCTGAATCGGGGCGACGATCAATGCCATCCACATGGCCATCGACAGCATCTTGCGTACAGCCGGGTTGTCACGCCCGCGCAGCAGGTGCCAGGCCGCCGAGGCACCGACGAAGAACGCGGTGGACACGAACGCGGCAGTAGCCATGTGCATCAGGCGGTAGGGGAAGGAGGG > NC_002947/5275926‑5276213 | gAACAGGATCAGTGGAGTCGGCTCACCGGGCACGTTCTCCCAGTGGCCTTCGATCGCAGCAATTTTCACCGGCTGGTGCTTGAGGGTATTGAGGCCGTGGAAATCGCCGATCACTGCCTGAATCGGGGCGACGATCAATGCCATCCACAt > 2:838338/1‑150 (MQ=255) gATCAGTGGAGTCGGCTCACCGGGCACGTTCTCCCAGTGGCCTTCGATCGCAGCAATTTTCACCGGCTGGTGCTTGAGGGTATTGAGGCCGTGGAAATCGCCGATCACTGCCTGAATCGGGGCGACGATCAATGCCATCCACATGGCCAt > 1:337088/1‑150 (MQ=255) gATCAGTGGAGTCGGCTCACCGGGCACGTTCTCCCAGTGGCCTTCGATCGCAGCAATTTTCACCGGCTGGTGCTTGAGGGTATTGAGGCCGTGGAAATCGCCGATCACTGCCTGAATCGGGGCGACGATCAATGCCATCCACATGGCCAt > 1:93879/1‑150 (MQ=255) tGGAGTCGGCTCACCGGGCACGTTCTCCCAGTGGCCTTCGATCGCAGCAATTTTCACCGGCTGGTGCTTGAGGGTATTGAGGCCGTGGAAATCGCCGATCACTGCCTGAATCGGGGCGACGATCAATGCCATCCACATGGCCATCGACAg > 2:340265/1‑150 (MQ=255) gCTCACCGGGCACGTTCTCCCAGTGGCCTTCGATCGCAGCAATTTTCACCGGCTGGTGCTTGAGGGTATTGAGGCCGTGGAAATCGCCGATCACTGCCTGAATCGGGGCGACGATCAATGCCATCCACATGGCCATCGACAGCATCTTGc < 2:337088/150‑1 (MQ=255) tCACCGGGCACGTTCTCCCAGTGGCCTTCGATCGCAGCAATTTTCACCGGCTGGTGCTTGAGGGTATTGAGGCCGTGGAAATCGCCGATCACTGCCTGAATCGGGGCGACGATCAATGCCATCCACATGGCCATCGACAGCATCTTGCGt > 1:495688/1‑150 (MQ=255) ttCGATCGCAGCAATTTTCACCGGCTGGTGCTTGAGGGTATTGAGGCCGTGGAAATCGCCGATCACTGCCTGAATCGGGGCGACGATCAATGCCATCCACATGGCCATCGACAGCATCTTGCGTACAGCCGGGTTGTCACGCCCGCgcag > 1:39244/1‑150 (MQ=255) tCGCAGCAATTTTCACCGGCTGGTGCTTGAGGGTATTGAGGCCGTGGAAATCGCCGATCACTGCCTGAATCGGGGCGACGATCAATGCCATCCACATGGCCATCGACAGCATCTTGCGTACAGCCGGGTTGTCACGCCCGCGCAGCAGGt < 1:838338/150‑1 (MQ=255) agcaATTTTCACCGGCTGGTGCTTGAGGGTATTGAGGCCGTGGAAATCGCCGATCACTGCCTGAATCGGGGCGACGATCAATGCCATCCACATGGCCATCGACAGCATCTTGCGTACAGCCGGGTTGTCACGCCCGCGCAGCAGGTGCCa > 2:785319/1‑150 (MQ=255) agcaATTTTCACCGGCTGGTGCTTGAGGGTATTGAGGCCGTGGAAATCGCCGATCACTGCCTGAATCGGGGCGACGATCAATGCCATCCACATGGCCATCGACAGCATCTGACGTACAGCCGGGTTGTCACGCCCGCGCAGCAGGTGCCa > 1:8500/1‑150 (MQ=255) ttttCACCGGCGGGTGCTTGAGGGTATTGAGGCCGTGGAAATCGCCGATCACTGCCTGAATCGGGGCGACGATCAATGCCATCCACATGGCCATCGACAGCATCTTGCGTACAGCCGGGTTGTCACGCCCGCGCAGCAGGTGCCAGgccg > 2:33370/1‑150 (MQ=255) aCCGGCTGGTGCTTGAGGGTATTGAGGCCGTGGAAATCGCCGATCACTGCCTGAATCGGGGCGACGATCAATGCCATCCACATGGCCATCGACAGCATCTTGCGTACAGCCGGGTTGTCACGCCCGCGCAGCAGGTGCCAGGCCGCCGAg > 1:717364/1‑150 (MQ=255) ggCTGGTGCTTGAGGGTATTGAGGCCGTGGAAATCGCCGATCACTGCCTGAATCGGGGCGACGATCAATGCCATCCACATGGCCATCGACAGCATCTTGCGTACAGCCGGGCTGTCACGCCCGCGCAGCAGGTGCCAGGCCGCCGAgtcc < 2:495688/150‑4 (MQ=255) ggTGCTTGAGGGTATTGAGGCCGTGGAAATCGCCGATCACTGCCTGAATCGGGGCGACGATCAATGCCATCCACATGGCCATCGACAGCATCTTGCGTACAGCCGGGTTGTCACGCCCGCGCAGCAGGTGCCAGGCCGCCGAGGCACcga < 2:342320/150‑1 (MQ=255) cTTGAGGGTATTGAGGCCGTGGAAATCGCCGATCACTGCCTGAATCGGGGCGACGATCAATGCCATCCACATGGCCATCGACAGCATCTTGCGTACAGCCGGGTTGTCACGCCCGCGCAGCAGGTGCCAGGCCGCCGAGGCACCGACgaa < 1:785319/150‑1 (MQ=255) aGGGTATTGAGGCCGTGGAAATCGCCGATCACTGCCTGAATCGGGGCGACGATCAATGCCATCCACATGGCCATCGACAGCATCTTGCGTACAGCCGGGTTGTCACGCCCGCGCAGCAGGTGCCAGGCCGCCGAGGCACCGACGAAGAAc > 2:682945/1‑150 (MQ=255) gTATTGAGGCCGTGGAAATCGCCGATCACTGCCTGAATCGGGGCGACGATCAATGCCATCCACATGGCCATCGACAGCATCTTGCGTACAGCCGGGTTGTCACGCCCGCGCAGCAGGTGCCAGGCCGCCGAGGCACCGACGAAGAAcgcg < 1:340265/150‑1 (MQ=255) aGGCCGTGGAAATCGCCGATCACTGCCTGAATCGGGGCGACGATCAATGCCATCCACATGGCCATCGACAGCATCTTGCGTACAGCCGGGTTGTCACGCCCGCGCAGCAGGTGCCAGGCCGCCGAGGCACCGACGAAGAACGCGGTGGac < 1:682945/150‑1 (MQ=255) tCGCCGATCACTGCCTGAATCGGGGCGACGATCAATGCCATCCACATGGCCATCGACAGCATCTTGCGTACAGCCGGGTTGTCACGCCCGCGCAGCAGGTGCCAGGCCGCCGAGGCACCGACGAAGAACGCGGTGGACACGAACGCGGCa < 2:717364/150‑1 (MQ=255) aaTCGGGGCGACGATCAATGCTATCCACATGGCCATCGACAGCATCTTGCGTACAGCCGGGTTGTCACGCCCGCGCAGCAGGTGCCAGGCCGCCGAGGCACCGACgaa < 1:118724/108‑1 (MQ=255) aaTCGGGGCGACGATCAATGCTATCCACATGGCCATCGACAGCATCTTGCGTACAGCCGGGTTGTCACGCCCGCGCAGCAGGTGCCAGGCCGCCGAGGCACCGACgaa > 2:118724/1‑108 (MQ=255) aaTCGGGGCGACGATCAATGCTATCCACATGGCCATCGACAGCATCTTGCGTACAGCCGGGTTGTCACGCCCGCGCAGCAGGTGCCAGGCCGCCGAGGCACCGACgaa > 2:118789/1‑108 (MQ=255) aaTCGGGGCGACGATCAATGCTATCCACATGGCCATCGACAGCATCTTGCGTACAGCCGGGTTGTCACGCCCGCGCAGCAGGTGCCAGGCCGCCGAGGCACCGACgaa < 1:118789/108‑1 (MQ=255) tCGTGGCGACGATCAATGCCATCCACATGGCCATCGACAGCATCTTGCGTACAGCCGGGTTGTCACGCCCGCGCAGCAGGTGCCAGGCCGCCGAGGCACCGACGAAGAACGCGGTGGACACGAACGCGGCAGTAGCCATGTGCATCAGGc < 1:33370/150‑1 (MQ=255) aTGCCATCCACATGGCCATCGACAGCATCTTGCGTACAGCCGGGTTGTCACGCCCGCGCAGCAGGTGCCAGGCCGCCGAGGCACCGACGAAGAACGCGGTGGACACGAACGCGGCAGTAGCCATGTGCATCAGGCGGTAGGGGAaggagg > 1:709735/1‑150 (MQ=255) tGCCATCCACATGGCCATCGACAGCATCTTGCGTACAGCCGGGTTGTCACGCCCGCGCAGCAGGTGCCAGGCCGCCGAGGCACCGACGAAGAACGCGGTGGACACGAACGCGGCAGTAGCCATGTGCATCAGGCGGTAGGGGAAGGAggg > 1:122418/1‑150 (MQ=255) | GAACAGGATCAGTGGAGTCGGCTCACCGGGCACGTTCTCCCAGTGGCCTTCGATCGCAGCAATTTTCACCGGCTGGTGCTTGAGGGTATTGAGGCCGTGGAAATCGCCGATCACTGCCTGAATCGGGGCGACGATCAATGCCATCCACATGGCCATCGACAGCATCTTGCGTACAGCCGGGTTGTCACGCCCGCGCAGCAGGTGCCAGGCCGCCGAGGCACCGACGAAGAACGCGGTGGACACGAACGCGGCAGTAGCCATGTGCATCAGGCGGTAGGGGAAGGAGGG > NC_002947/5275926‑5276213 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |