| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,329,444 | T→A | 10.2% | L10Q (CTG→CAG) | PP_4690 → | Rieske 2Fe‑2S family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,329,444 | 0 | T | A | 10.2% | 134.0 / 4.7 | 47 | L10Q (CTG→CAG) | PP_4690 | Rieske 2Fe‑2S family protein |
| Reads supporting (aligned to +/- strand): ref base T (24/18); new base A (2/3); total (26/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTTGTGGCGAGCGGCGGAACTGCGCACCATAGGCAGCCGGGCGCAGAGAATGCGATCAATTTGATAATTATTCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCGGGACATCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGATGACAGCG > NC_002947/5329296‑5329593 | ccTTGTGGCGAGCGGCGGAACTGCGCACCATAGGCAGCCGGGCGCAGAGAATGCGATCAATTTGATAATTATTCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTg > 1:357270/1‑150 (MQ=255) gAGCGGCGGAACTGCGCACCATAGGCAGCCGGGCGCAGAGAATGCGATCAATTTGATAATTATTCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGc > 1:89869/1‑150 (MQ=255) aGCGGCGGAACTGCGCACCATAGGCAGCCGGGCGCAGAGAATGCGATCAATTTGATAATTATTCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCAGGCCGAgggat < 1:568647/150‑3 (MQ=255) aaCTGCGCACCATAGGCAGCCGGGCGCAGAGAATGCGATCAATTTGATAATTATTCGCATTGGAGCGTCAAGCCATCAACAACAGCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCcgc > 1:270905/1‑150 (MQ=255) cgcACCATAGGCAGCCGGGCGCAGAGAATGCGATCAAGTTGATAATTATTCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCtt > 2:127960/1‑150 (MQ=255) aGGCAGCCGGGCGCAGAGAATGCGATCAATTTGATAATTATTCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAg < 1:59973/150‑1 (MQ=255) gCAGCCGGGCGCAGAGAATGCGATCAATTTGATAATTATTCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGAc > 1:470564/1‑150 (MQ=255) gcAGAGAATGCGATCAATTTGATAATTATTCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAAc > 2:442343/1‑150 (MQ=255) agaATGCGATCAATTTGATAATTATTCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGaa < 1:439838/145‑1 (MQ=255) agaATGCGATCAATTTGATAATTATTCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGaa > 2:439838/1‑145 (MQ=255) aTCAATTTGATAATTATTCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCGCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGgcgtgc > 1:634298/1‑150 (MQ=255) aaTTTGATAATTATTCGAATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTc > 1:730440/1‑150 (MQ=255) aTTTGATAATTATTCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCAGGCCGAgg > 1:330038/1‑98 (MQ=255) aTTTGATAATTATTCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCAGGCCGAgg < 2:330038/98‑1 (MQ=255) ttGATAATTATTCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTGCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGcc < 2:406258/150‑1 (MQ=255) gATAATTATTCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCaa < 1:442343/150‑1 (MQ=255) ttattCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAAc > 2:679065/1‑125 (MQ=255) ttattCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAAc < 1:679065/125‑1 (MQ=255) ttCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACCTCCTTTGTACATCCCTGGGCGTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGgt < 1:319147/150‑1 (MQ=255) ggAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGtgtacctgt > 1:717255/1‑150 (MQ=255) tCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCa > 2:89837/1‑150 (MQ=255) tCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCa > 2:89834/1‑150 (MQ=255) aaGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAAc < 2:270905/150‑1 (MQ=255) tCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGc < 1:221585/150‑1 (MQ=255) tCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGc > 1:184329/1‑150 (MQ=255) gtTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCAc > 1:819830/1‑150 (MQ=255) tttCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGACGAGGGCCAAAGCGGCGCCTTCAGCGTCGCCGGCCTCGAACTGTTCGGCATGCGTCGCCAAGACACGGTGTCCCTGTAACGCAACCGCTGCGCGCaac > 2:366679/1‑148 (MQ=255) aCGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCGGGa < 1:58500/150‑1 (MQ=255) gggTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCTTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCGGGACa < 1:127960/150‑1 (MQ=255) aTGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCGGGACATCCCGCTGAACTgg < 2:717255/150‑1 (MQ=255) cTTCCTTTGTACATCCCGCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCGGGACATCCCGCTGAACTGGGCaga < 2:634298/150‑1 (MQ=255) cTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCgg > 1:376261/1‑71 (MQ=255) cTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCgg < 2:376261/71‑1 (MQ=255) ggcccTCGGCCAGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGc < 1:389367/104‑1 (MQ=255) ggcccTCGGCCAGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGc > 2:389367/3‑106 (MQ=255) aTCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGc > 1:322208/1‑106 (MQ=255) aTCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGc > 1:322434/1‑106 (MQ=255) aTCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGc < 2:322434/106‑1 (MQ=255) aTCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGc < 2:322208/106‑1 (MQ=255) aTCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCGGGACATCCCGCTGAACTGGGCAGAGGACGCGTTTCt > 2:712396/1‑150 (MQ=255) ccTGGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCGGGACATCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGa < 2:104158/150‑1 (MQ=255) ccTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCGGGACATCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGa > 2:498770/1‑150 (MQ=255) ccTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCGGGACATCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGa > 2:558616/1‑150 (MQ=255) cTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCGGGACATCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGAt > 1:703466/1‑150 (MQ=255) cGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCGGGACATCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGATGa < 2:470564/150‑1 (MQ=255) gCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCGGGACATCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGATGACa > 1:272243/1‑150 (MQ=255) cTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCGGGACATCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGATGACAgc < 2:89869/150‑1 (MQ=255) tGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCg > 2:31349/1‑85 (MQ=255) tGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCg < 1:31349/85‑1 (MQ=255) tGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCGGGACATCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGATGACAgct < 1:166419/150‑2 (MQ=255) | CCTTGTGGCGAGCGGCGGAACTGCGCACCATAGGCAGCCGGGCGCAGAGAATGCGATCAATTTGATAATTATTCGCATTGGAGCGTCAAGCCATCACGTGTTTCACGGGTCATCCTTGCCATGCACTTCCTTTGTACATCCCTCGGCCTGGCCGAGGGCCAAAGCCGCGCCTTCAGCGTAGACGGCCTCGAACTGTTCGGCGTGCGTCGCCAAGGCCAGGTGTACCTGTACCGCAACCGCTGCCCGCACCGGGACATCCCGCTGAACTGGGCAGAGGACGCGTTTCTGGATGACAGCG > NC_002947/5329296‑5329593 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |