| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,346,316 | T→G | 13.1% | Y556D (TAC→GAC) | acsA‑II → | acetyl‑CoA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,346,316 | 0 | T | G | 13.1% | 105.3 / 11.6 | 46 | Y556D (TAC→GAC) | acsA‑II | acetyl‑CoA synthetase |
| Reads supporting (aligned to +/- strand): ref base T (22/18); new base G (2/4); total (24/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.32e-01 | |||||||||||
CTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTC > NC_002947/5346168‑5346458 | ctGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTa > 1:729731/1‑150 (MQ=255) gATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGc < 2:259912/150‑1 (MQ=255) gATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGc < 2:164264/150‑1 (MQ=255) aCCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTc > 2:322033/1‑150 (MQ=255) aCCGGGCGCATCGACGATGTGATCAACGTGTACGGCAATCTCTTCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTAGACGCCttg < 2:839644/150‑2 (MQ=255) ccGGGCGCATCGACGATGTGATCAACGTGTCAGGTCACCGCATCGGCACCGCTGCCCCAGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTAGACGCCttgg < 1:438197/150‑3 (MQ=255) acgaTGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGCAAGTGCACTGATGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCGAGGTCCAGGGCGTCTACGCCGTCGTCACGACCAtca > 1:141647/1‑148 (MQ=255) aCGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGc < 2:678385/121‑1 (MQ=255) aCGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGc > 1:678385/1‑121 (MQ=255) gtgtCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCa < 1:516082/150‑1 (MQ=255) gccACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGCCCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGaca < 2:271500/148‑1 (MQ=255) gCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGc > 2:508462/1‑133 (MQ=255) gCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGc < 1:508462/133‑1 (MQ=255) aCCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCg > 2:191216/1‑132 (MQ=255) aCCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCg < 1:191216/132‑1 (MQ=255) ccGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCa > 1:284295/1‑116 (MQ=255) ccGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCa < 2:284295/116‑1 (MQ=255) aTCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTc > 2:528833/1‑108 (MQ=255) aTCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTc < 1:528833/108‑1 (MQ=255) tGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAATCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAgctgct < 1:184231/150‑1 (MQ=255) tGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAgctgct > 1:249161/1‑150 (MQ=255) gAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTg < 2:104836/150‑1 (MQ=255) cATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGATCAGCAAGGAAATCGGc > 1:261487/1‑150 (MQ=255) aaGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACAATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCAGCAGCTTCGCCAAGcccg > 2:74878/1‑148 (MQ=255) gCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCa > 1:55592/1‑121 (MQ=255) gCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCa < 2:55592/121‑1 (MQ=255) gtggtCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGAtt < 2:729731/150‑1 (MQ=255) tCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGACTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAgg > 2:390964/1‑149 (MQ=255) gCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTggg > 1:350343/1‑150 (MQ=255) tACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTgg > 2:442580/1‑94 (MQ=255) tACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTgg < 1:442580/94‑1 (MQ=255) cACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAgcg > 2:172655/1‑150 (MQ=255) gACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTa > 2:391840/1‑150 (MQ=255) cccAAGCGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGCTGCAACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTAccc > 2:481800/3‑150 (MQ=255) cTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTAccc > 2:627628/1‑150 (MQ=255) cAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCaa < 1:627628/150‑1 (MQ=255) aaGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGAt > 1:160692/1‑125 (MQ=255) aaGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGAt < 2:160692/125‑1 (MQ=255) gCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGAcgc < 1:481800/150‑1 (MQ=255) gaaGGCGTAGACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGgc < 2:506370/127‑1 (MQ=255) gaaGGCGTAGACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGgc > 1:506370/2‑128 (MQ=255) gaaGGCGTAGACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGgc < 1:752842/127‑1 (MQ=255) gaaGGCGTAGACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGgc > 2:752842/2‑128 (MQ=255) aGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGGGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTc > 2:289659/1‑150 (MQ=255) gCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGcc > 2:713804/1‑59 (MQ=255) gCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGcc < 1:713804/59‑1 (MQ=255) | CTGGATCACCGGGCGCATCGACGATGTGATCAACGTGTCCGGGCACCGCATCGGCACCGCTGAAGTGGAAAGTGCACTGGTGCTGCATGACAGCGTGGCCGAAGCCGCGGTGGTCGGCTACCCCCACGACCTCAAGGGCCAAGGCGTCTACGCCTTCGTCACGACCATGAATGGCGTGACACCAGATGACACGCTCAAGGCCGAGCTGCTGGCCCTGGTCAGCAAGGAAATCGGCAGCTTCGCCAAGCCTGAGCTGATTCAGTGGGCGCCAGCGCTACCCAAGACGCGCTC > NC_002947/5346168‑5346458 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |