| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,382,317 | A→T | 16.1% | E124D (GAA→GAT) | smpB → | SsrA‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,382,317 | 0 | A | T | 16.1% | 100.6 / 13.8 | 44 | E124D (GAA→GAT) | smpB | SsrA‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (18/18); new base T (3/4); total (22/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.22e-01 | |||||||||||
CCCACGTCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTCGCGG > NC_002947/5382172‑5382460 | cccACGTCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCg > 2:710792/1‑150 (MQ=255) ccACGTCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCACAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGc > 2:200111/1‑150 (MQ=255) aCGTCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGATTTCGCAc < 1:491095/150‑1 (MQ=255) gTCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGATTTCGCACtt < 1:508401/150‑2 (MQ=255) gCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTTGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGAGCAAGTCCGAAATCGCACTGGGCAAGGGCAAGAAGGAAt > 1:516392/1‑150 (MQ=255) gCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCAGATAAGGTGAGAAATCGCACTGGGCAAGGGCAAGAAGGAAt > 2:649194/1‑150 (MQ=255) cAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACaa < 1:710792/150‑1 (MQ=255) tgctgctgAACAAGCGCGAGCTCGAGCGGATGGAAGCGGCCGTGACGCAAAAAGGCTACAGCTGTGTGGGTTTGGCGCTGTCCTGGAGCAAGCACCTGATCAAGTGCGACATCGCACTGGGCAAGGGCAAGAGGGAATTCGACAAGcgcg > 1:417386/1‑150 (MQ=255) tgAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGAGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCa < 1:649194/150‑1 (MQ=255) gAACAAGCGCGAGCTCGAGCGCGTGGAAGAGGCCGTGGCGCAAAAAGGCTACACCTGAGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCAt > 1:534178/1‑150 (MQ=255) gAACAAGCGCGAGCTCGAGCGCGTGCCCGGTGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCAt > 1:450684/1‑150 (MQ=255) gcgcGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGa > 1:668218/1‑150 (MQ=255) tCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGAtt < 2:326481/150‑1 (MQ=255) tCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGAtt > 2:468525/1‑150 (MQ=255) cGAGCGCGTGCAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTc < 1:210314/150‑1 (MQ=255) gcgcGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCaa > 1:229399/1‑150 (MQ=255) gAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAg < 2:575091/150‑1 (MQ=255) aGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCaagaag > 2:44985/1‑100 (MQ=255) aGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCaagaag < 1:44985/100‑1 (MQ=255) cGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCaagaag < 1:7238/98‑1 (MQ=255) cGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCaagaag > 2:7238/1‑98 (MQ=255) gCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTgggc < 1:387490/84‑1 (MQ=255) gCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTgggc > 2:387490/1‑84 (MQ=255) gcgcAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGCACGAGATTCCAACCGCGAGCTGCAGCGGGCt > 1:641587/1‑150 (MQ=255) cTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACaa > 2:444541/1‑150 (MQ=255) cTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGa > 1:607178/1‑75 (MQ=255) cTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGa < 2:607178/75‑1 (MQ=255) tgGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGc > 2:108239/1‑127 (MQ=255) tgGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGc < 1:108239/127‑1 (MQ=255) ggCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAaga < 1:468525/150‑1 (MQ=255) ggCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAaga < 2:668218/150‑1 (MQ=255) cTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGt < 2:740230/150‑1 (MQ=255) cTGTAATGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTActc < 1:200111/150‑1 (MQ=255) cggcccTGATCAAGTGCCAAACCGAACTGGGTAAGGGCAAGCAGGAATTCGACAAGCGCGACACCATGCGTCAACGAGATTCCAACCGCGATGTGCAGCGGGCTGTGCGGAAAAAGGGCAAGGAAGAGTAACTTCTCTTCAACCTGtgac < 2:516392/146‑4 (MQ=255) aCCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCaa > 2:325159/1‑82 (MQ=255) aCCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCaa < 1:325159/82‑1 (MQ=255) cTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGaa < 2:623565/120‑1 (MQ=255) cTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGaa > 1:623565/1‑120 (MQ=255) tCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGAAGAGCCGTGGACCAGGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTc < 2:450684/150‑1 (MQ=255) ccaGTGCGATTTCGCACTTGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCaa < 2:198508/109‑1 (MQ=255) ccaGTGCGATTTCGCACTTGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCaa > 1:198508/3‑111 (MQ=255) ccaGTGCGATTTCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTcg > 2:235343/3‑150 (MQ=255) gTGCGATTTCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTCGCgg > 2:153099/1‑150 (MQ=255) gTGCGATTTCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTCGCgg < 1:153099/150‑1 (MQ=255) | CCCACGTCATCGCCGACCCTACACGCACCCGCAAGCTGCTGCTGAACAAGCGCGAGCTCGAGCGCGTGGAAGCGGCCGTGGCGCAAAAAGGCTACACCTGTGTGGCTTTGGCGCTGTACTGGAGCAAGCACCTGATCAAGTGCGAAATCGCACTGGGCAAGGGCAAGAAGGAATTCGACAAGCGCGACACCATGCGCGAACGCGATTCCAACCGCGAGCTGCAGCGGGCTGTGCGGAACAAGGGCAAGGAAGAGTAACTACTCTTCAACCTGTTCCGGCCTCTTCGCGG > NC_002947/5382172‑5382460 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |