Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,427,343 T→C 8.0% N270D (AAT→GAT)  rhlE‑I ← ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,427,3430TC 8.0% 138.4 / 2.8 50N270D (AAT→GAT) rhlE‑IATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base T (20/26);  new base C (2/2);  total (22/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.06e-01

TGACGACCAGCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCT‑GCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTCCACAGGCACCAGCCAC  >  NC_002947/5427199‑5427488
                                                                                                                                                |                                                                                                                                                  
tGACGACCAGCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGAATGCCGACGGCATCCACgg                                                                                                                                               <  2:290080/150‑2 (MQ=255)
    gacCAGCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTc                                                                                                                                           <  1:442387/150‑1 (MQ=255)
    gacCAGCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTc                                                                                                                                           >  2:138239/1‑150 (MQ=255)
     acCAGCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGTGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCg                                                                                                                                          <  2:535134/150‑1 (MQ=255)
       cAGCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGTGCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGc                                                                                                                                        >  2:670272/1‑150 (MQ=255)
         gCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCa                                                                                                                                      <  2:460171/150‑1 (MQ=255)
            gCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCa                                                                                                                                   >  1:267610/1‑150 (MQ=255)
            gCAAGCCCTAGAAATCCACGCCACGGGTCGTCACAACGGAGGCCACCAACACTTGCACCGCACGGGCCTTGAAGCTGTCCAGCGCACGCTAGCGCGTTGCCTATAGGCGGTCACTGTTGATGCCGTCGGCATTCACGCCTTCGGCCAGcc                                                                                                                                   >  2:138036/1‑149 (MQ=255)
             cAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCaa                                                                                                                                  <  2:267610/150‑1 (MQ=255)
                  cATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGc                                                                                                                                        >  1:263912/1‑139 (MQ=255)
                  cATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGc                                                                                                                                        <  2:263912/139‑1 (MQ=255)
                    tCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTcc                                                                                                                           >  2:552002/1‑150 (MQ=255)
                          tCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGc                                                                                                                     <  2:813036/150‑1 (MQ=255)
                             aagCCACGGGCCGCCCCATCGGTCGCCACCCCCACTTGCACCTCACGGGCCTGGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTCTCGGCGGTAAACGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGa                                                                                                                  <  1:283894/148‑1 (MQ=255)
                                  aCGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGAc                                                                                                             <  1:288922/150‑1 (MQ=255)
                                   cGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACg                                                                                                            <  2:500714/150‑1 (MQ=255)
                                    gggCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGc                                                                                                           <  1:121888/150‑1 (MQ=255)
                                            acaTCGGTCGCCACCAGCCCTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCATCAGCTGATCGACGCCATTGCgg                                                                                                   <  1:295966/150‑1 (MQ=255)
                                            acaTCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCgg                                                                                                   <  1:760872/150‑1 (MQ=255)
                                              aTCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGt                                                                                                 >  2:60944/1‑150 (MQ=255)
                                                    cGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGc                                                                                           <  1:346140/150‑1 (MQ=255)
                                                    cGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGc                                                                                           <  2:850603/150‑1 (MQ=255)
                                                       caccaGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTTGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGaa                                                                                        >  2:737816/1‑150 (MQ=255)
                                                         ccaGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAaca                                                                                      >  2:293892/1‑150 (MQ=255)
                                                          caGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCAc                                                                                                                                                 <  1:477110/90‑1 (MQ=255)
                                                          caGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCAc                                                                                                                                                 >  2:477110/1‑90 (MQ=255)
                                                                    aCCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGc                                                                                                                     <  1:19631/108‑1 (MQ=255)
                                                                    aCCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGc                                                                                                                     >  2:19631/1‑108 (MQ=255)
                                                                     ccTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCt                                                                          <  2:314579/150‑1 (MQ=255)
                                                                       tCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTc                                                                        <  1:737816/150‑1 (MQ=255)
                                                                               cTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTgg                                                                                            >  2:454322/1‑122 (MQ=255)
                                                                               cTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTgg                                                                                            <  1:454322/122‑1 (MQ=255)
                                                                                    aGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCCCCTGCTTCCAGCGCT‑GCTTgc                                                          >  2:16453/1‑150 (MQ=255)
                                                                                         tCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGTTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCT‑GCTTgcgccca                                                     <  1:670272/150‑4 (MQ=255)
                                                                                         tCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCT‑GCTTGCgcagc                                                     >  2:287209/1‑150 (MQ=255)
                                                                                               gcACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCT‑GCTTGCGCAGCAGGTgg                                               <  1:138239/150‑1 (MQ=255)
                                                                                                   gCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGa                                                                                                                  <  1:208657/80‑1 (MQ=255)
                                                                                                   gCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGa                                                                                                                  >  2:208657/1‑80 (MQ=255)
                                                                                                                cTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCT‑GCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCtt                              >  1:313035/1‑150 (MQ=255)
                                                                                                                     ggCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCgct‑gc                                                              >  1:164663/1‑113 (MQ=255)
                                                                                                                     ggCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCgct‑gc                                                              <  2:164663/113‑1 (MQ=255)
                                                                                                                        ggTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGTAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTATTGGCGAACACCAGTACCTGCTTCCAGCGCT‑GCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGcttct                      <  1:138036/150‑1 (MQ=255)
                                                                                                                             gcGTGAATGCCGACGGCATCCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCT‑GCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTcc                 >  1:176598/2‑150 (MQ=255)
                                                                                                                             ccGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCT‑GCTTGCGCAGCAGGTGGCAGAAc                                         <  2:284654/126‑1 (MQ=255)
                                                                                                                             ccGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCT‑GCTTGCGCAGCAGGTGGCAGAAc                                         >  1:284654/1‑126 (MQ=255)
                                                                                                                               gTGAATGCCGACGGCATCCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCT‑GCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTCcac               >  2:95978/1‑150 (MQ=255)
                                                                                                                                  gATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGAGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTCGC‑CGATCGAGATGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTCCACAgg            >  2:450678/1‑150 (MQ=255)
                                                                                                                                       cGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCT‑GCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTCCACAGGCACCa       >  1:96184/1‑150 (MQ=255)
                                                                                                                                         tCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCT‑GCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTCCACAGGCACCAGc     <  2:313035/150‑1 (MQ=255)
                                                                                                                                            gCATCCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCT‑GCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTCCACAGGCACCAGCCAc  <  1:95978/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
TGACGACCAGCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCT‑GCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTCCACAGGCACCAGCCAC  >  NC_002947/5427199‑5427488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑