| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,430,424 | A→G | 14.0% | intergenic (‑58/+103) | PP_4769 ← / ← PP_4770 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,430,424 | 0 | A | G | 14.0% | 114.9 / 7.1 | 48 | intergenic (‑58/+103) | PP_4769/PP_4770 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (21/20); new base G (3/4); total (24/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTGTAGTTGAAATGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCA > NC_002947/5430283‑5430566 | ggTGTAGTTGAAATGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCTAGCGGTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCg < 2:69504/150‑1 (MQ=255) ttGAAATGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCcac > 2:619426/1‑150 (MQ=255) ttGAAATGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCcac > 2:383074/1‑150 (MQ=255) tGAAATGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCcaca < 1:546800/150‑1 (MQ=255) agtgaccagggcggatacgccgacggtggttttggcagcgatgacggtgggttcttctcggacgacgattcttacgtctgacaggtctggcatactgctggcatttccgggggcgctttgcgccCCTTCGC‑G‑GACACGCCCGCTCCcaca > 2:616445‑M1/125‑150 (MQ=255) gAAATGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAg < 1:300990/150‑1 (MQ=255) cAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATcgc > 2:72714/1‑150 (MQ=255) ggcggatacgccgacggtggttttggcagcgatgacggtgggttcttctcggacgacgattcttacgtctgacaggtctggcatactgctggcatttccgggggcgctttgcgccCCTTCGC‑G‑GGCACGCCCGCTCCCACAGGTATcgcg < 2:785261‑M1/35‑1 (MQ=255) gCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATcgcgc < 1:331895/150‑1 (MQ=255) gggCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATATTTTTCCCCTGGGTCCCTCCTTTTTGGCCTGGAGTGCCGGGGCGCTGCTGCGTAGACCTTCGC‑T‑GACACGCCCGCTCCCACAGGTATCGCGCGTaca < 2:524510/150‑1 (MQ=255) acggtggttttggcagcgatgacggtgggttcttctcggacgacgattcttacgtctgacaggtctggcatactgctggcatttccgggggcgctttgcgccCCTTCGC‑G‑GGCACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTgg > 1:785261‑M1/103‑150 (MQ=255) cggtggttttggcagcgatgacggtgggttcttctcggacgacgattcttacgtctgacaggtctggcatactgctggcatttccgggggcgctttgcgccCCTTCGC‑G‑GGCACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTggg > 1:588843‑M1/102‑150 (MQ=255) ggTGTAGTCGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTGCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCgg > 2:423448/1‑150 (MQ=255) tgtAGTAGAGTCTCTGCAGATCGGCGAACGGCGCGATATTCTTCCCCTGCTCGCCTCATTTCTGGTCTGGAGTGCGGGGCGGCTGCTGCGCAGCCCTTCGC‑G‑GGCACGCCCGCTGCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGc < 2:264456/150‑1 (MQ=25) gagGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAAACTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCcg > 1:296176/1‑150 (MQ=255) gTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACGGGt < 2:427308/113‑1 (MQ=255) gTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACGGGt > 1:427308/1‑113 (MQ=255) ggCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAg > 1:55621/1‑150 (MQ=255) ttctcggacgacgattcttacgtctgacaggtctggcatactgctggcatttccgggggcgctttgcgccCCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGa < 1:629671‑M1/50‑1 (MQ=255) ttctcggacgacgattcttacgtctgacaggtctggcatactgctggcatttccgggggcgctttgcgccCCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGa > 2:629671‑M1/71‑120 (MQ=255) aaCAGTGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGcc < 1:253522/150‑1 (MQ=255) aaCAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGcc < 2:445802/150‑1 (MQ=255) cAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGcccc < 2:55621/150‑1 (MQ=255) gcgcGATCTTTTTCATCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGCGCTG‑‑ACGTCTTCCTCGGCAGCGGTACGCCCGCTCCCACGGGTATCGAGCGTACACCCGTGGGTGCGTTCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCg < 1:619426/150‑1 (MQ=255) ttcttacgtctgacaggtctggcatactgctggcatttccgggggcgctttgcgccCCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGt < 2:702077‑M1/46‑1 (MQ=255) ttcttacgtctgacaggtctggcatactgctggcatttccgggggcgctttgcgccCCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGt > 1:702077‑M1/57‑102 (MQ=255) ttttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCca < 1:383973/150‑1 (MQ=255) ttttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCca < 2:18816/150‑1 (MQ=255) ttttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCca > 1:235698/1‑150 (MQ=255) ttacgtctgacaggtctggcatactgctggcatttccgggggcgctttgcgccCCTTCGC‑G‑GGCACGCCCGCTCCCACAGGTATcgcgcg < 2:55225‑M1/37‑1 (MQ=255) ttacgtctgacaggtctggcatactgctggcatttccgggggcgctttgcgccCCTTCGC‑G‑GGCACGCCCGCTCCCACAGGTATcgcgcg > 1:55225‑M1/54‑90 (MQ=255) aCCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGt > 1:84612/1‑150 (MQ=255) aCCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGt > 1:373302/1‑150 (MQ=255) gggAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGc > 1:350426/1‑150 (MQ=255) gAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGGGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCa > 1:687050/1‑150 (MQ=255) cTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGAcc > 1:143592/1‑150 (MQ=255) tCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCt < 1:183475/150‑1 (MQ=255) ttttGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCa < 2:143592/150‑1 (MQ=255) gCCTGGAGGGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGt > 1:573332/1‑150 (MQ=255) cGGGGGGCGGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAAtcgtcg > 2:80897/1‑150 (MQ=255) ggggggCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATcgtcgt < 2:235698/150‑1 (MQ=255) gggggCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCgtcgtc > 2:760113/1‑150 (MQ=255) ggggCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTCCACACGTGGGAGAGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTcc < 1:383074/150‑1 (MQ=255) ggCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGACAGCAGTATGCTAGACCTGTCAGACGTACGAATCGTAGTCCga > 1:256626/1‑150 (MQ=255) tgcGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAgaagaa < 1:760113/150‑1 (MQ=255) gcAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAAcc < 2:588791/149‑1 (MQ=255) gcAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAAcc > 1:588791/1‑149 (MQ=255) TTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCa < 2:430604‑M1/150‑1 (MQ=255) | GGTGTAGTTGAAATGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGC‑G‑GACACGCCCGCTCCCACAGGTATCGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCA > NC_002947/5430283‑5430566 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |