| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,434,888 | T→G | 10.3% | N142H (AAC→CAC) | hrpB ← | ATP‑dependent helicase HrpB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,434,888 | 0 | T | G | 10.3% | 90.6 / 3.8 | 39 | N142H (AAC→CAC) | hrpB | ATP‑dependent helicase HrpB |
| Reads supporting (aligned to +/- strand): ref base T (15/20); new base G (2/2); total (17/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.87e-01 | |||||||||||
CCCCAGCGGATATCCACCGGATGCATGCGGCCTTCGCTGCTGACCACCGGGGCATCGTCCAGCAACCGCGACAGGCGTTCGCCTTCCAGGGTCGCGGACATCAGCAGAATCTTCAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGCCCACACCTTCCAGCGCCGGGTCGGCTTGCAGGCGGCGTGTGAGGATGCCTTCGGTCACCACTTCGA > NC_002947/5434740‑5435022 | ccccAGCGGATATCCACCGGATGCATGCGGCCTTCGCTGCTGACCACCGGGGCATCGTCCAGCAACCGCGACAGGCGTTCGCCTTCCAGGGTCGCGGACATCAGCAGAATCTTCAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTa > 1:201680/1‑150 (MQ=255) aGCGGATATCCACCGGATGCATGCGGCCTTCGCTGCTGACCACCGGGGCATCGTCCAGCAACCGCGACAGGCGTTCGCCTTCCAGGGTCGCGGACATCAGCAGAATCTTCAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGc > 2:375121/1‑150 (MQ=255) gCGGATATCCACCGGATGCATGCGGCCTTCGCTGCTGACCACCGGGGCATCGTCCAGCAACCGCGACAGGCGTTCGCCTTCCAGGGTCGCGGACATCAGCAGAATCTTCAGTGGCGGGTCGTCACGCAGAAACTCGCGGCCGTTAAGGCt > 2:514805/1‑150 (MQ=255) aCCGGATGCATGCGGCCTTCGCTGCTGACCACCGGGGCATCGTCCAGCAACCGCGACAGGCGTTCGCCTTCCAGGGTCGCGGACATCAGCAGAATCTTCAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGt < 1:759449/150‑1 (MQ=255) ccGGATGCATGCGGCCTTCGCTGCTGACCACCGGGGCATCGTCCAGCAACCGCGACAGGCGTTCGCCTTCCAGGGTCGCGGACATCAGCAGAATCTTCAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTg > 2:269382/1‑150 (MQ=255) atgcGGCCTTCGCTGCTGACCACCGGGGCATCGTCCAGCAACCGCGACAGGCGTTCGCCTTCCAGGGTCGCGGACATCAGCAGAATCTTCAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCg < 1:204822/150‑1 (MQ=255) ttCGCTGCTGACCACCGGGGCATCGTCCAGCAACCGCGACAGGCGTTCGCCTTCCAGGGTCGCGGACATCAGCAGAATCTTCAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGc > 2:641206/1‑135 (MQ=255) ttCGCTGCTGACCACCGGGGCATCGTCCAGCAACCGCGACAGGCGTTCGCCTTCCAGGGTCGCGGACATCAGCAGAATCTTCAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGc < 1:641206/135‑1 (MQ=255) cGCTGCTGACCACCGGGGCATCGTCCAGCAACCGCGACAGGCGTTCGCCTTCCAGGGTCGCGGACATCAGCAGAATCTTCAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGc > 1:535025/1‑150 (MQ=255) gACCACCGGGGCATCGTCCAGCAACCGCACAAAGAGTTCTCCTGTCAGGTTCTCGGACATCAGCAGAACTTTAGGTCGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTc < 1:405337/150‑1 (MQ=255) aTCGTCCAGCAACCGCGACAGGCGTTCGCCTTCCAGGGTCGCGGACATCAGCAGAATCTTCAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATtcgtc < 1:243623/150‑1 (MQ=255) aTCGTCCAGCAACCGCGACAGGCGTTCGCCTTCCAGGGTCGCGGACATCAGCAGAATCTTCAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATtcgtc < 1:269382/150‑1 (MQ=255) aTCGTCCAGCAACCGCGACAGGCGTTCGCCTTCCAGGGTCGCGGACATCAGCAGAATCTTCAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCCGCGCTCGTGAAATtcgtc < 2:535025/150‑1 (MQ=255) aaCCGCGACAGGCGTTCGCCTTCCAGGGTCGCGGACATCAGCAGAATCTTCAGTGGCGGGGCGTCGCGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATcagc > 1:383151/1‑150 (MQ=255) gcgACAGGCGTTCGCCTTCCAGGGTCGCGGACATCAGCAGAATCTTCAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGc < 2:771500/150‑1 (MQ=255) gcgACAGGCGTTCGCCTTCCAGGGTCGCGGACATCAGCAGAATCTTCAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGc > 2:643798/1‑150 (MQ=255) ttCGCCTTCCAGGGTCGCGGACATCAGCAGAATCTTCAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGCCCACACCTTc < 1:779830/150‑1 (MQ=255) tCCAGGGTCGCGGACATCAGCAGAATCTTCAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGCCCACACCTTCCAGCGcc < 1:375121/150‑1 (MQ=255) cgGACATCAGCAGAATCTTCAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGCCCACACCTTCCAGCGCCGGGTCGGCtt < 2:746439/150‑1 (MQ=255) gACATCAGCAGAATCTTCAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGCCCACACCTTCCAGCGCCGGGTCGGCTTGc < 2:563761/150‑1 (MQ=255) cATCAGCAGAATCTTCAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGCCCACACCTTCCAGCGCCGGGTCGGCTTCCAg < 2:461818/150‑1 (MQ=255) aTCAGCAGAATCTTCAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGCCCACACCTTCCAGCGCCTGGTCGGCt < 2:423455/144‑1 (MQ=255) aTCAGCAGAATCTTCAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGCCCACACCTTCCAGCGCCGGGTCGGCt > 1:423455/1‑144 (MQ=255) cagcagAATCTTCAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGCCCACACCTTCCAGCGCCGGGTCGGCTTGCAggcg > 2:454246/1‑150 (MQ=255) gAATCTTCAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTTGAAGATCAGCAGGCCCACACCTTCCAGCGCCGGGTCGGCTTGCAGGCGGCgtg > 1:415559/1‑150 (MQ=255) tCAGTGGCGGGTCGTCACTCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTGGTGAAATTCGTCGAAAATCAGCAGGCCCACACCTTCCAGCGCCGGGTCGCCTTCCAGGCGGCGAGtgatga > 2:348145/1‑150 (MQ=255) cAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGCCCACACCTTCCAGCGCCGGGTCGGCTTGCAGGCGGCGTGTGAGGAt < 1:734339/150‑1 (MQ=255) aGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGCCCACACCTTCCAGCGCCGGGTCGGCTTGCAGGCGGCGTGTGAGGATg > 2:46234/1‑150 (MQ=255) gCGGGTCGTCACGCAGCAACTCGCGGCCGTGAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGCCcaca < 1:716973/104‑1 (MQ=255) gCGGGTCGTCACGCAGCAACTCGCGGCCGTGAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGCCcaca > 2:294539/1‑104 (MQ=255) gCGGGTCGTCACGCAGCAACTCGCGGCCGTGAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGCCcaca > 2:716973/1‑104 (MQ=255) gCGGGTCGTCACGCAGCAACTCGCGGCCGTGAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGCCcaca < 1:294539/104‑1 (MQ=255) gggTCGTCACGCAGCAACTCGCCGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGCCCACACCTTCCAGCGCCGGGTCGGCTTGCAGGCGGCGTGTGAGGATGCCTTCg < 1:680955/150‑1 (MQ=255) tcgtcACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGCCCACACCTTCCAGCGCCgg < 2:682259/113‑1 (MQ=255) tcgtcACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGCCCACACCTTCCAGCGCCgg > 1:682259/1‑113 (MQ=255) tcgtcACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGCCCACACCTTCCAGCGCCGGGTCGGCTTGCAGGCGGCGTGTGAGGAt < 1:570115/140‑1 (MQ=255) tcgtcACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGCCCACACCTTCCAGCGCCGGGTCGGCTTGCAGGCGGCGTGTGAGGAt > 2:570115/1‑140 (MQ=255) gcagcaACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGCCCACACCTTCCAGCGCCGGGTCGGCTTGCAGGCGGCGTGTGAGGATGCCTTCGGTCACCACtt < 1:643798/150‑1 (MQ=255) gcaACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGCCCACACCTTCCAGCGCCGGGTCGGCTTGCAGGCGGCGTGTGAGGATGCCTTCGGTCACCACTTCGa < 2:644583/150‑1 (MQ=255) | CCCCAGCGGATATCCACCGGATGCATGCGGCCTTCGCTGCTGACCACCGGGGCATCGTCCAGCAACCGCGACAGGCGTTCGCCTTCCAGGGTCGCGGACATCAGCAGAATCTTCAGTGGCGGGTCGTCACGCAGCAACTCGCGGCCGTTAAGGCTCAGCGCAAGTGCGAGATCGGCATCCAGGCTGCGCTCGTGAAATTCGTCGAAGATCAGCAGGCCCACACCTTCCAGCGCCGGGTCGGCTTGCAGGCGGCGTGTGAGGATGCCTTCGGTCACCACTTCGA > NC_002947/5434740‑5435022 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |