Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,467,345 G→T 9.1% G183G (GGC→GGA)  rlpA ← RlpA‑like lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,467,3450GT 9.1% 131.1 / 3.4 44G183G (GGC→GGA) rlpARlpA‑like lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (20/20);  new base T (2/2);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01

GACCCGCCCGGTACTGGCCGGTATTGCCTGGGTCTGCGCCACTTGCGGCTCCTTCAGCACCATCGGCGGCGTTTGGCCGCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCGATGATTCGGTCGGAATAGAACGGGCCACGGTCGTTCACCCGAAGGATCACGCTGCGGCCGTTCGCCAGGTTGGTCACCCGCACATAGGCTGGCAGCGG  >  NC_002947/5467213‑5467491
                                                                                                                                    |                                                                                                                                                  
gACCCGCCCGGTACTGGCCGGTATTGCCTGGGTCTGCGCCACTTGCGGCTCCTTCAGCACCATCGGCGGCGTTTGGCCGCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGcc                                                                                                                                   >  1:653148/1‑150 (MQ=255)
     gcccgGTACTGGCCGGTATTGCGTGGGTCTGCGCCACTTGCGGCTCCTTCAGCACCATCGGCGGCGTTTGGCCGCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCt                                                                                                                              >  2:740127/1‑150 (MQ=255)
       ccgGTACTGGCCGGTATTGCCTGGGTCTGTGCCACTTGCGGCTCCTTCAGCACCATCGGCGGCGTTTGGCCGCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCttt                                                                                                                            <  2:42693/150‑1 (MQ=255)
              tGGCCGGTATTGCCTGGGTCTGCGCCACTTGCGGCTCCTTCAGCACCATCGGCGGCGTTTGGCCGCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGcc                                                                                                                                                  >  1:442682/1‑121 (MQ=255)
              tGGCCGGTATTGCCTGGGTCTGCGCCACTTGCGGCTCCTTCAGCACCATCGGCGGCGTTTGGCCGCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGcc                                                                                                                                                  <  2:442682/121‑1 (MQ=255)
                gCCGTTATTGCCTGGGTCTGCGCCACTTGCGGCTCCTTCAGCACCATCGGCGGCGTTTGGCCGCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGgca                                                                                                                   <  2:774934/150‑1 (MQ=255)
                  cGGTATTGCCTGGGTCTGCGCCACTTGCGGCTCCTTCAGCACCATCGGCGGCGTTTGGCCGCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGgcagc                                                                                                                 >  1:417920/1‑150 (MQ=255)
                  cGGTATTGCCTGGGTCTGCGCCACTTGCGGCTCCTTCAGCACCATCGGCGGCGTTTGGCCGCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGgcagc                                                                                                                 >  2:584625/1‑150 (MQ=255)
                        tGCCTGGGTCTGCGCCACTTGCGGCTCCTTCAGCACCATCGGCGGCGTTTGGCCGCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGa                                                                                                           <  2:359905/150‑1 (MQ=255)
                        tGCCTGGGTCTGCGCCACTTGCGGCTACTTCAGCACCAGCGGCGTCGTTTGGCCTCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTTGCGGCAGCATAGGa                                                                                                           <  2:7791/150‑1 (MQ=255)
                           cTGGGTCTGCGCCACTTGCGGCTCCTTCAGCACCATCGGCGGCGTTTGGCCGCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACaa                                                                                                        >  2:6021/1‑150 (MQ=255)
                           agttgTCTGCGCCACTTGCGGCTCCTTCAGCACCATCGGCGGCGTTTGGCCGCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACaa                                                                                                        >  1:670318/5‑150 (MQ=255)
                                    cgcCACTTGCGGCTCCTTCAGCACCATCGGCGGCGTTTGGCCGCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCgatgat                                                                                               <  1:534679/150‑1 (MQ=255)
                                     gcCACTTGCGGCTCCTTCAGCACCATCGGCGGCGTTTGGCCGCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCGATGAtt                                                                                              <  1:703690/150‑1 (MQ=255)
                                      ccACTTGCGGCTCCTTCAGCACCATCGGCGGCGTTTGGCCGCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCGATGATtc                                                                                             >  2:679874/1‑150 (MQ=255)
                                         cTTGCGGCTCCTTCAGCACCATCGGCGGCGTTTGGCCGCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCACCGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGa                                                                                                           >  1:695713/1‑133 (MQ=255)
                                         cTTGCGGCTCCTTCAGCACCATCGGCGGCGTTTGGCCGCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCAACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGa                                                                                                           <  2:695713/133‑1 (MQ=255)
                                          ttGCGGCTCCTTCAGCACCATCGGCGGCGTTTGGCCGCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCGATGATtcggtc                                                                                         >  2:260/1‑150 (MQ=255)
                                              ggCTCCTTCAGCACCATCGGCGGCGTTTGGCCGCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCGATGATTCGGTCGGaa                                                                                     <  2:670318/150‑1 (MQ=255)
                                                     tCAGCACCATCGGCGGCGTTTGGCCGCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCGATGATTCGGTCGGAATAGAACg                                                                              <  1:679874/150‑1 (MQ=255)
                                                                  cggcgTTTGGCCGCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCGATGGTTCGGTCGGAATAGAACGGGCCACGGTCGtt                                                                 <  2:526950/150‑1 (MQ=255)
                                                                      gTTTGGCCGCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCGATGATTCGGTCGGAATAGAACGGGCCACGGTCGTTCAcc                                                             >  1:266276/1‑150 (MQ=255)
                                                                            ccGCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCCGCATAGGACAAGTCGATGATTCGGTCGGAATAGAACGGGCCACGGTCGTTCACCCGAAgg                                                       >  2:339218/1‑150 (MQ=255)
                                                                                       ccaccaCTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCGATGATTCGGTCGGAATAGAACGGGCCACGGTCGTTCACCCGAAGGATCACGCTGCg                                            >  1:799279/1‑150 (MQ=255)
                                                                                         accacTGCTGCGGATCGATACCCTCGACGCGCACATGCGCTGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCGATGATTCGGTCGGAATAGAACGGGCCACGGTCGTTCACCCGAAGGATCACGCTGCGGc                                          >  2:598908/1‑150 (MQ=255)
                                                                                           cacTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCGATGATTCGGTCGGAATAGAACGGGCCACGGTCGTTCACCCGAAGGATCACGCCGCGGCCg                                        >  2:23330/1‑150 (MQ=255)
                                                                                                     gATCGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCGATGATTCGGTCGGAATAGAACGGGCCACGGTCGTTCACCCGAAGGATCACGCTGCGGCCGTTCGCCAGGt                              <  1:584625/150‑1 (MQ=255)
                                                                                                        cGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCGATGATTCGGTCGGAATAGAACGGGCCACGGTCGTTCACCCGAAGGATCACGCTGCGGCCGTTCGCCAGGTTgg                           <  2:653148/150‑1 (MQ=255)
                                                                                                         gATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCGATGATTCGGTCGGAATAGAACGGGCCACGGTCGTTCACCCGAAGGATCACGCTGCGGCCGTTCGCCAGGTTGGt                          <  1:374879/150‑1 (MQ=255)
                                                                                                          aTACCCTCGACGCGCACATGCGCGGTTCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCga                                                                                                   >  1:678873/1‑76 (MQ=255)
                                                                                                          aTACCCTCGACGCGCACATGCGCGGTTCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCga                                                                                                   <  2:679111/76‑1 (MQ=255)
                                                                                                          aTACCCTCGACGCGCACATGCGCGGTTCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCga                                                                                                   <  2:678873/76‑1 (MQ=255)
                                                                                                          aTACCCTCGACGCGCACATGCGCGGTTCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCga                                                                                                   >  1:679111/1‑76 (MQ=255)
                                                                                                              ccTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCgatga                                                                                                <  2:692306/75‑1 (MQ=255)
                                                                                                              ccTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAACCGAGCTTTTTCGCGGCAGCATAGGACAAGTCgatga                                                                                                >  1:692306/1‑75 (MQ=255)
                                                                                                               ggcaccgcgCATGTGCGCGGTGCCGATCTCGGCATAGCCGTGCTTTTTCGCGGCAGCATAGGACAAGTCGATGATTAGGTCGGAATAGAACGGGCCACGGTCGTTCACCCGAAGGATCACGCTGCGGCCGTTCGCCAGGTTGGTCACCCg                    >  1:69364/6‑150 (MQ=255)
                                                                                                               cTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCGATGATtcggtc                                                                                         <  2:226866/81‑1 (MQ=255)
                                                                                                               cTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCGATGATtcggtc                                                                                         >  1:226866/1‑81 (MQ=255)
                                                                                                                     gcgcACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCGATGATTCGGTCGGAATAGAACgg                                                                             >  2:468148/1‑87 (MQ=255)
                                                                                                                     gcgcACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCGATGATTCGGTCGGAATAGAACgg                                                                             <  1:468148/87‑1 (MQ=255)
                                                                                                                       gcACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATa                                                                                                              >  2:661943/1‑52 (MQ=255)
                                                                                                                       gcACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATa                                                                                                              <  1:661943/52‑1 (MQ=255)
                                                                                                                        cacaTGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACACGTCTATTATTCGGTCGGAATAGAACGGGCCACGGTCGTTCACCCGAAGGATCACGCTGCGGCCGTTCGCCAGGTTGGTCACCCGCACATAGGc           <  1:740127/150‑1 (MQ=255)
                                                                                                                                 ggTGCCGGTCTCGGCATTGCCGAGCTTTTGCGCGGCAGCATAGGACAAGTCGATGATTCGGTCGGAATAGAACGGGCCACGGTCGTTCACCCGAAGGATCACGCTGCGGCCGTTCGCCAGGTTGGTCACCCGCACATAGGCTGGCAGCgg  <  2:266276/150‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                                  
GACCCGCCCGGTACTGGCCGGTATTGCCTGGGTCTGCGCCACTTGCGGCTCCTTCAGCACCATCGGCGGCGTTTGGCCGCGCTGAGCCCACCACTGCTGCGGATCGATACCCTCGACGCGCACATGCGCGGTGCCGATCTCGGCATAGCCGAGCTTTTTCGCGGCAGCATAGGACAAGTCGATGATTCGGTCGGAATAGAACGGGCCACGGTCGTTCACCCGAAGGATCACGCTGCGGCCGTTCGCCAGGTTGGTCACCCGCACATAGGCTGGCAGCGG  >  NC_002947/5467213‑5467491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑