Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,566,467 T→A 8.7% Q357L (CAG→CTG)  mutL ← DNA mismatch repair protein MutL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,566,4670TA 8.7% 129.7 / 4.1 48Q357L (CAG→CTG) mutLDNA mismatch repair protein MutL
Reads supporting (aligned to +/- strand):  ref base T (22/20);  new base A (2/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

GCTGCGAGGGACGTGGGGTGTATTGATACTGGTAACCGGCGCCGCTGCCACCATTGGAAAACGACTGTTGCGGGGCTCGAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACGTACATCGGCCAAGGCACGGTGCAAGGTGCCATACAGGAAGTCATGCACCGAGCGCCCTTCGCGGAACCGTACTTCATGCTTGGTTGGG  >  NC_002947/5566324‑5566616
                                                                                                                                               |                                                                                                                                                     
gCTGCGAGGGACGTGGGGTGTATTGATACTGGTAACCGGCGCCGCTGCCACCATTGGAAAACGACTGTTGCGGGGCTCGAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTGGCGCCCGAACTCACCGGCCTGCTGGCCgg                                                                                                                                                 >  2:288919/1‑150 (MQ=255)
  tGCGAGGGACGTGGGGTGTATTGATACTGGTAACCGGCGCCGCTGCCACCATTGGAAAACGACTGTTGCGGGGCTCGAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTg                                                                                                                                               >  1:543915/1‑150 (MQ=255)
   gCGAGGGACGTGGGGTGTATTGATACTGGTAACCGGCGCCGCTGCCACCATTGGAAAACGACTGTTGCGGGGCTCGAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCAGCAGGCCGGTga                                                                                                                                              <  1:235794/150‑2 (MQ=255)
     gAGGGACGTGGGGTGTATTGATACTGGTAACCGGCGCCGCTGCCACCATTGGAAAACGACTGTTGCGGGGCTCGAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTTACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTgggg                                                                                                                                            <  1:812071/150‑1 (MQ=255)
               gggTGTATTGATACTGGTAACCGGCGCCGCTGCCACCATTGGAAAACGACTGTTGCGGGGCTCGAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCt                                                                                                                                  <  1:386787/150‑1 (MQ=255)
                 gtgtATTGATACTGGTAACCGGCGCCGCTGCCACCATTGGAAAACGACTGTTGCGGGGCTCGAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCGCa                                                                                                                                >  2:124924/1‑150 (MQ=255)
                      ttGATACTGGTAACCGGCGCCGCTGCCACCATTGGAAAACGACTGTTGCGGGGCTCGAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGt                                                                                                                             <  1:522892/148‑1 (MQ=255)
                      ttGATACTGGTAACCGGCGCCGCTGCCACCATTGGAAAACGACTGTTGCGGGGCTCGAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGt                                                                                                                             >  2:522892/1‑148 (MQ=255)
                        gATACTGGTAACCGGCGCCGCTGCCACCATTGGAAAACGACTGTTGCGGGGCTCGAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTAAGGTCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACgg                                                                                                                         >  1:269635/1‑150 (MQ=255)
                               gTAACCGGCGCCGCTGCCACCATTGGAAAACGACTGTTGCGGGGCTCGAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAgg                                                                                                                  >  2:269801/1‑150 (MQ=255)
                               gTAACCGGCGCCGCTGCCACCATTGGAAAACGACTGTTGCGGGGCTCGAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAgg                                                                                                                  <  1:269801/150‑1 (MQ=255)
                                 aaCCGGCGCCGCTGGCACCATTGGAAAACTACTGTTGCGGGGCTCGGGGCTGATCAAGCACCGGCGAGGCCAGGCGCCTTTCACACTGGTGCCCGAACTAACCGACCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTg                                                                                                                >  2:206744/1‑150 (MQ=255)
                                  aCCGGCGCCGCTGCCACCATTGGAAAACGACTGTTGCGGGGCTCGACGCTGTCCAAGCATCCGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGACCTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGc                                                                                                               <  1:327230/150‑1 (MQ=255)
                                     gtcgccgcTGTCCCCAGTAGAAAACGGCTTTTGCGGGGCTCGAGGCTGTTCAAGCACCGGCGAGCTCAGGCCCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGc                                                                                                            <  1:189531/148‑1 (MQ=255)
                                              gCCACCATTGGAAAACGACTGTTGCGGGGCTCGAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTc                                                                                                   <  1:681254/150‑1 (MQ=255)
                                                caccaTTGGAACACGACTGTTGCGGGGCGGGAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCCTTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCCGGTACGGCCGCAGGCGCGGCCAGCTGGTCtt                                                                                                 >  1:51088/1‑150 (MQ=255)
                                                   caTTGGAAAACGACTGTTGCGGGGCTCGAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCg                                                                                              >  2:87471/1‑150 (MQ=255)
                                                       ggAAAACGACTGTTGCGGGGCTCGAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACg                                                                                          <  1:87471/150‑1 (MQ=255)
                                                       ggAAAACGACTGTTGCGGGGCTCGAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACg                                                                                          >  1:490198/1‑150 (MQ=255)
                                                        gAAAACGACTGTTGCGGGGCTCGAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACGt                                                                                         <  2:704227/150‑1 (MQ=255)
                                                            aCGACTGTTGCGGGGCTCGAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACGTACAt                                                                                     <  2:543915/150‑1 (MQ=255)
                                                                            tCGAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAAc                                                                                                                                       <  2:834167/84‑1 (MQ=255)
                                                                            tCGAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAAACAGCTCAGGTACGGCAGCCGGTGCGGCCAGCTGGTCTTCCGGACGTACATCGGCCAAGGCACGGTg                                                                     >  2:601975/1‑150 (MQ=255)
                                                                            tCGAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCCGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAAc                                                                                                                                       >  1:834167/1‑84 (MQ=255)
                                                                              gAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACGTACATCGGCCAAGGCACg                                                                        >  1:126397/1‑145 (MQ=255)
                                                                              gAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACGTACATCGGCCAAGGCACg                                                                        <  2:126397/145‑1 (MQ=255)
                                                                                ggCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACGTACATCGGCCAACGCACGGTGCAAg                                                                 >  1:550168/1‑150 (MQ=255)
                                                                                  cTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACGTACATCGGCCAAGGCACGGTGCAAGGt                                                               <  1:288919/150‑1 (MQ=255)
                                                                                   tGTTCAAGCACCGGCGAGGCACCGCGCAATTCACCCTGGGGGCCGGATCCACCTTGCTACGGGCCGTGCTGTGGAAGCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACGTACATCGGCCAAGGCACTGTACAAGGTg                                                              <  2:53192/150‑1 (MQ=255)
                                                                                    gTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACGTACATCGGCCAAGGCACGGTGCAAGGTGc                                                             <  2:490198/150‑1 (MQ=255)
                                                                                          gCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCAGCAGGCCGGt                                                                                                                                                >  1:239019/1‑61 (MQ=38)
                                                                                          gCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCAGCAGGCCGGt                                                                                                                                                <  2:239019/61‑1 (MQ=38)
                                                                                                gCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACGTACATCGGCCAAGGCACGGTGCAAGGTGCCATACAGGAAGt                                                 >  1:162772/1‑150 (MQ=255)
                                                                                                   aGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACGTACATCGGCCAAGGCAc                                                                         <  1:578879/123‑1 (MQ=255)
                                                                                                   aGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACGTACATCGGCCAAGGCAc                                                                         >  2:578879/1‑123 (MQ=255)
                                                                                                     gCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGTCAGCTGGTCTTCCGGACGTACATCGGCCAAGGCACGGTGCAAgg                                                                <  1:371437/130‑1 (MQ=255)
                                                                                                     gCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGTCAGCTGGTCTTCCGGACGTACATCGGCCAAGGCACGGTGCAAgg                                                                >  2:371437/1‑130 (MQ=255)
                                                                                                     gCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACGTACATCGGcc                                                                                <  2:529684/114‑1 (MQ=255)
                                                                                                     gCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACGTACATCGGcc                                                                                >  1:529684/1‑114 (MQ=255)
                                                                                                                      cTGGGGCCGGATCTTACCGGCGTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGTTGGTCTTCCGGACGTACATCGGCCAAGGCACGGTGCAAGGCGCCATACAGGAAGTCATGCACCGAGCGCCCTTcgcg                           <  2:201706/150‑1 (MQ=255)
                                                                                                                             ccGAACTCACCGGCCTACTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACGTACATCGGCCAAGGCACGGTGCAAGGTGCCATACAGGAAGTCATGCACCGAGCGCCCTTCGCGGAACCGt                    >  1:380472/1‑150 (MQ=255)
                                                                                                                                   ccACCGGCCAGCAGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACGTACATCGGCGAAGGCACGGTGCAAGGTGCCATACAGGAAGTCATGCACCGAGCGCCCTTCGCGGAACCGTACTTCa              >  2:26680/2‑150 (MQ=255)
                                                                                                                                     aCCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACGTACATCGGCCAAGGCACGGTGCAAGGTGCCATACAGGAAGTCATGCACCGAGCGCCCTTCGCGGAACCGTACTTCATg            >  2:807310/1‑150 (MQ=255)
                                                                                                                                     aCCGGCCAGCCCGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGACTTTCGCACTTACATCCGCCAAGGCACGGTGCAAGGTGCCATACAGGAAGTCATGCACCGAGCGCCCTTCGCGGAACCGTACTTCATg            <  1:488124/150‑1 (MQ=255)
                                                                                                                                        ggCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACGTACATCGGCCAAGGCACGGTGCAAGGTGCCATACAGGAAGTCATGCACCGAGCGCCCTTCGCTGAACCGTACTTCATGCtt         <  2:162772/150‑1 (MQ=255)
                                                                                                                                        ggCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACGTACATCGGCCAAGGCACGGTGCAAGGTGCCATACAGGAAGTCATGCACCGAGCGCCCTTCGCGGAACCGTACTTCATGCtt         >  1:764181/1‑150 (MQ=255)
                                                                                                                                         gCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGt                                                                                                                 >  2:423249/1‑45 (MQ=255)
                                                                                                                                         gCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGt                                                                                                                 <  1:423249/45‑1 (MQ=255)
                                                                                                                                            tgctgGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACGTACATCGGCCAAGGCACGGTGCAAGGTGCCATACAGGAAGTCATGCACCGAGCGCCCTTCGCGGAACCGTACTTCATGCttggtt     >  1:4484/1‑150 (MQ=255)
                                                                                                                                              ctgGCCTGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACGTACATCGGCCAAGGCACGGTGCAAGGTGCCATACAGGAAGTCATGCACCGAGCGCCCTTCGCGGAACCGTACTTCATGCttggttgg   <  2:380472/150‑1 (MQ=255)
                                                                                                                                              ctgGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACGTACATCGGCCAAGGCACGGTGCAAGGTGCCATACAGGAAGTCATGCACCGAGCGCCCTTCGCGGAACCGTACTTCATGCttggttgg   <  2:550168/150‑1 (MQ=255)
                                                                                                                                               tgGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACGTACATCGGCCAAGGCACGGTGCAAGGTGCCATACAGGAAGTCATGCACCGAGCGCCCTTCGCGGAACCGTACTTCATGCTTGGTTggg  <  2:4484/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
GCTGCGAGGGACGTGGGGTGTATTGATACTGGTAACCGGCGCCGCTGCCACCATTGGAAAACGACTGTTGCGGGGCTCGAGGCTGTTCAAGCACCGGCGAGGCCAGGCGCATTTCACCCTGGGGCCCGAACTCACCGGCCTGCTGGCCGGTGGGGCGAACCAGCTCAGGTACGGCAGCAGGTGCGGCCAGCTGGTCTTCCGGACGTACATCGGCCAAGGCACGGTGCAAGGTGCCATACAGGAAGTCATGCACCGAGCGCCCTTCGCGGAACCGTACTTCATGCTTGGTTGGG  >  NC_002947/5566324‑5566616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑