| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,567,230 | A→T | 15.4% | L103M (TTG→ATG) ‡ | mutL ← | DNA mismatch repair protein MutL |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,567,230 | 0 | A | T | 15.4% | 62.4 / 5.3 | 26 | L103M (TTG→ATG) ‡ | mutL | DNA mismatch repair protein MutL |
| Reads supporting (aligned to +/- strand): ref base A (11/11); new base T (2/2); total (13/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
CCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGCCATCATCCCGCACCTTCAGCA > NC_002947/5567089‑5567367 | ccACCGAGGTACCGACCGGGTGCGCCGCCGGTTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCTTGGCCTCga < 1:34102/150‑2 (MQ=255) aCCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAAGGCTCACTGGCACTGGCGGTACGCGAGGTAAGGGTCAGGGGCCCCACCCAACTGATCCAGGCCAAGGCCTCGCCa > 1:484332/1‑150 (MQ=255) tACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGACAGGCAAAGAGCACACGTGTGGTATGCGGGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGc > 1:530779/1‑150 (MQ=255) gCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTa > 2:142212/1‑150 (MQ=255) cgcgGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCt > 2:536683/1‑150 (MQ=255) gcgGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCtt < 2:803403/150‑1 (MQ=255) ttCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCtggt < 1:142212/150‑1 (MQ=255) ttCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCgtg < 1:202788/150‑1 (MQ=255) cTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAg > 1:533627/1‑150 (MQ=255) ctggcGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGt > 1:516809/1‑94 (MQ=255) ctggcGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGt < 2:516809/94‑1 (MQ=255) cGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCgg < 1:822518/150‑1 (MQ=255) gtcagggtcaggCGCGCCACCGAACTGATCGAGGCCTTGGCCTCgat < 2:2815/47‑3 (MQ=37) gtcagggtcaggCGCGCCACCGAACTGATCGAGGCCTTGGCCTCgat > 1:2815/1‑45 (MQ=255) agggtcaggCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTg > 1:273208/1‑150 (MQ=255) gtcaggCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGCca < 2:484332/150‑1 (MQ=255) aaCTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCtggtgg > 1:657713/1‑84 (MQ=255) aaCTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCtggtgg < 2:657713/84‑1 (MQ=255) aaCTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCt < 1:454963/79‑1 (MQ=255) aaCTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGCCatcatc < 1:4699/139‑1 (MQ=255) aaCTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGCCatcatc > 2:4699/1‑139 (MQ=255) aaCTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGAGCTTGCt > 2:454963/1‑79 (MQ=255) aCTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGCCATCATCCCGCACCTTcag < 2:273208/150‑1 (MQ=255) gtggcGAGGCCTTGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGCCATCATCCCGCACCTTcagca > 2:41058/5‑150 (MQ=255) cAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAg > 2:766519/1‑90 (MQ=255) cAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAg < 1:766519/90‑1 (MQ=255) | CCACCGAGGTACCGACCGGGTGCGCCGCCGGCTGCACCCGCGGCGTCATGTCGCGGCCTTCGGTTTCTACTTGCCAGGCCTCACTGGCACTGGCGGTACGCGAGGTCAGGGTCAGGCGCGCCACCGAACTGATCGAGGCCAAGGCCTCGCCACGGAAGCCCAGGCTCAATACCCCTTCTAGGTCTTCCAGCTCGCGGATCTTGCTGGTGGCGTGTCGAGCCAGGGCCAGCGGCAGGTCGTCGGCAGAAATGCCGCTGCCATCATCCCGCACCTTCAGCA > NC_002947/5567089‑5567367 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |