| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,573,801 | C→T | 15.3% | G278G (GGC→GGT) | rsgA → | ribosome biogenesis GTPase RsgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,573,801 | 0 | C | T | 15.3% | 89.0 / 8.1 | 39 | G278G (GGC→GGT) | rsgA | ribosome biogenesis GTPase RsgA |
| Reads supporting (aligned to +/- strand): ref base C (18/15); new base T (3/3); total (21/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
AGCCTGCTGCCAGACGCTGGCACCCGCGTCGGCGACTTGTCGGAATGGTCAGGCCAGGGTACCCACACCACCACCACGGCGCGGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAG > NC_002947/5573652‑5573945 | aGCCTGCTGCCAGACGCTGGCACCCGCGTCGGCGACTTGTCGGAATGGTCAGGCCAGGGTACCCACACCACCACCACGGCGCGGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGc < 1:675530/150‑1 (MQ=255)aGCCTGCTGCCAGACGCTGGCACCCGCGTCGGCGACTTGTCGGAATGGTCAGGCCAGGGTACCCACACCACCACCACGGCGCGGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGc < 1:675451/150‑1 (MQ=255) ctgctgCCAGACGCTGGCACCCGCGTCGGCGACTTGTCGGACTGGCGAGGGGAGGATTCCCACACCACCACAACGCCGCGGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGCCAt < 1:406065/150‑1 (MQ=255) gctgcCAGACGCTGGCACCCGCGTCGGCGACTTGTCGGAATGGTCAGGCCAGGGTACCCACACCACCACCACGGCGCGGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGCCATGt < 2:541544/150‑1 (MQ=255) tGGCACCCGCGTCGGCGACTTGTCGGAATGGTCAGGCCAGGGTACCCACACCACCACCACGGCGCGGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGcgacga < 2:28657/150‑1 (MQ=255) ccGCGTCGGCGACTTGTCGGAATGGTCAGGCCAGGGTACCCACACCACCACCACGGCGCGGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTgga > 1:403514/1‑150 (MQ=255) gTCGGCGACTTGGCGGAAGGGTCAGGCCAGGGCACCCAACCCACCACCACGGCGCGGCTTTACCACTGCCCCAATGGCGGGGACCTGATAGACT‑CGCCGGGGATTCGCGAGCTCGGCCTTGGCCATGTCAGCCGTGACGACGTGGAGGAt < 2:95582/150‑1 (MQ=255) gCGACTTGTCGGAATGGTCAGGCCAGGGTACCCACACCACCACCACGGCGCGGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGCGATGTGAGCCGCGACGGCGTGGAGGATGGCt > 2:624077/1‑150 (MQ=255) aGGCCAGGGTACCCACACCACCACCACGGCGCGGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGTAGGATGGCTTCATCGAGTTCCGCGACCt > 1:525492/1‑150 (MQ=255) cAGGGTACCCACACCACCACCACGGCGCGGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTc > 1:591704/1‑150 (MQ=255) atggTACCCACACCACCACCACGGCGCGGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCg < 1:563783/148‑1 (MQ=255) gggTACCCACACCACCACCACGGCGCGGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCgg > 1:302882/1‑150 (MQ=255) ccACACCACCACCACGGCGCGGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCt < 2:641131/119‑1 (MQ=255) ccACACCACCACCACGGCGCGGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCt > 1:641131/1‑119 (MQ=255) ccACACCACCACCACGGCGCGGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTc > 1:258937/1‑121 (MQ=255) ccACACCACCACCACGGCGCGGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTc < 2:258937/121‑1 (MQ=255) acacCACCACCACGGCGCGGCTTTACCACTTCCCCAATGGCGGGGACCAGAT‑AACTGCACCTGGTATTCCGGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGcc > 1:531943/1‑150 (MQ=255) accaccaccacGGCGCGGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGc > 1:310101/1‑150 (MQ=255) gcgcGGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGTCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTc > 2:135905/1‑126 (MQ=255) gcgcGGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGTCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTc > 2:135998/1‑126 (MQ=255) gcgcGGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGTCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTc < 1:135998/126‑1 (MQ=255) gcgcGGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGTCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTc < 1:135905/126‑1 (MQ=255) gcgcGGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGTCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGCTCCGCGACCTGTTc < 1:135908/126‑1 (MQ=255) gcgcGGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGTCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGCTCCGCGACCTGTTc > 2:135908/1‑126 (MQ=255) cgGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAg < 2:302882/150‑1 (MQ=255) cTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCAt < 2:403514/150‑1 (MQ=255) ccAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCgg < 1:624077/150‑1 (MQ=255) gggACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGTAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGATTGCAAGCATGACCGCGAACCGGGCTgcgcgc < 2:525492/150‑1 (MQ=255) gATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCggg > 2:354798/1‑135 (MQ=255) gATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCggg < 1:354798/135‑1 (MQ=255) gggTATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGAt > 2:668936/1‑52 (MQ=255) gggTATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGAt < 1:668936/52‑1 (MQ=255) gggTATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAaga > 2:291798/1‑150 (MQ=255) ttCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGgc > 2:560140/1‑150 (MQ=255) ttCGCGAGTTCGGCCTTCGCAATGTCCGGCGCCACTACCTGGAAGATGCCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGgc < 2:310101/150‑1 (MQ=255) gcgAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCa > 1:237484/1‑150 (MQ=255) cgAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCAc < 2:757669/134‑1 (MQ=255) cgAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCAc > 1:757669/1‑134 (MQ=255) gAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATc > 1:71767/1‑150 (MQ=255) ggCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCg > 2:71700/1‑150 (MQ=255) cTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAg > 1:487355/1‑150 (MQ=255) | AGCCTGCTGCCAGACGCTGGCACCCGCGTCGGCGACTTGTCGGAATGGTCAGGCCAGGGTACCCACACCACCACCACGGCGCGGCTTTACCACTTCCCCAATGGCGGGGACCTGATCGACT‑CGCCGGGTATTCGCGAGTTCGGCCTTGGCCATGTCAGCCGCGACGACGTGGAGGATGGCTTCATCGAGTTCCGCGACCTGTTCGGCACCTGCCGCTTCCGCGACTGCAAGCATGACCGCGAACCGGGCTGCGCGCTGCTCAAGGCACTGGAAGAGGGGCGCATCAAGCCGCAG > NC_002947/5573652‑5573945 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |