| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,585,562 | A→T | 8.0% | V74D (GTC→GAC) | parC ← | DNA topoisomerase IV subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,585,562 | 0 | A | T | 8.0% | 133.1 / 2.2 | 47 | V74D (GTC→GAC) | parC | DNA topoisomerase IV subunit A |
| Reads supporting (aligned to +/- strand): ref base A (20/23); new base T (2/2); total (22/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01 | |||||||||||
GGCGAACGACTTCGGATCATCCGGCGCACCCCAGTTGCCCTGGCCATCGACCAGGGTATAGCGGTAGCTGAACGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGTAGTTGAGGTAG > NC_002947/5585414‑5585711 | ggcgAACGACTTCGGATCATCCGGCGCACCCCAGTTGCCCTGGCCATCGACCAGGGTATAGCGGTAGCTGAACGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGAc > 1:737783/1‑150 (MQ=255) gACTTCGGATCATCCGGCGCACCCCAGTTGCCCTGGCCATCGACCAGGGTATAGCGGTAGCTGAACGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGt > 2:353450/1‑150 (MQ=255) ttCGGATCATCCGGCGCACCCCAGTTGCCCTGGCCATCGACCAGGGTATAGCGGTAGCTGAACGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGcc < 1:124629/150‑1 (MQ=255) ttCGGATCATCCGGCGCACCCCAGTTGCCCTGGCCATCGACCAGGGTATAGCGGTAGCTGAACGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGcc > 2:125834/1‑150 (MQ=255) cGGATCATCCGGCGCACCCCAGTTGCCCTGGCCATCGACCAGGGTATAGCGGTAGCTGAACGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGa < 1:250659/150‑1 (MQ=255) tcCGGCGCACCCCAGTTGCCCTGGCCATCGACCAGGGTATAGCGGTAGCTGAACGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTtg < 2:513728/150‑1 (MQ=255) gcACCCCAGTTGCCCTGGCCATCGACCAGGGTATAGCGGTAGCTGAACGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTg < 2:737783/150‑1 (MQ=255) gcACCCCAGTTGCCCTGGCCATCGACCAGGGTATAGCGGTAGCTGAACGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTg < 1:5534/150‑1 (MQ=255) aCCCCAGTTGCCCTGGCCATCGACCAGGGTATAGCGGTAGCTGAACGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGTGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCtt < 1:689468/147‑1 (MQ=255) aCCCCAGTTGCCCTGGCCATCGACCAGGGTATAGCGGTAGCTGAACGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCtt > 2:689468/1‑147 (MQ=255) cccAGTTGCCCTGGCCATCGACCAGGGTATAGCGGTAGCTGAACGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTggcat > 2:89140/1‑150 (MQ=255) cccAGTTGCCCTGGCCATCGACCAGGGTATAGCGGTAGCTGAACGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTggcat > 1:168923/1‑150 (MQ=255) ccTGGCCATCGACCAGGGTATAGCGGTAGCTGAACGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTggcatcggagatcg > 1:188313/1‑144 (MQ=255) cTGGCCATCGACCAGGGTATAGCGGTAGCTGAACGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCt < 2:342265/134‑1 (MQ=255) cTGGCCATCGACCAGGGTATAGCGGTAGCTGAACGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCt > 1:342265/1‑134 (MQ=255) tGGCCATCGACCAGGGTATAGCGGTAGCTGAACGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGc > 1:498270/1‑150 (MQ=255) tGGCCATCGACCAGGGTATAGCGGTAGCTGAACGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGc < 2:326723/150‑1 (MQ=255) gCCATCGACCAGGTTATAGCGGTAGCGGAACGGTTGCGCCATCAGCACCATGGCCTCGTAGCAGTCCTAATCTCCATGGGGATGAAACTTGCCGAGCACGTCACCGACGGT‑ACGTTCCTACTTCTTGTGCTTGGCATCGGCATCGAGccc < 1:47345/150‑1 (MQ=255) ccAGGGTATAGCGGTAGCTGAACGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAAATTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGCCCCAACTCGc > 1:277746/1‑150 (MQ=255) aGGGTATAGCGGTAGCTGAACGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGc < 1:204664/120‑1 (MQ=255) aGGGTATAGCGGTAGCTGAACGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGc > 2:204664/1‑120 (MQ=255) ataGCGGTAGCTAAACGGCTGCGCCAGCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGGGCCCCAACTCGCTCATGGc < 2:263931/150‑1 (MQ=255) gCGGTAGCTGAACGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTa < 2:516499/150‑1 (MQ=255) cTGAACGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATg > 1:772248/1‑150 (MQ=255) aaCGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTggcatcgg > 1:257400/1‑112 (MQ=255) aaCGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTggcatcgg < 2:257400/112‑1 (MQ=255) gCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCt < 2:277746/150‑1 (MQ=255) gcgcCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCa > 2:459409/1‑150 (MQ=255) aTCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGc > 2:373565/1‑150 (MQ=255) tCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCt < 1:541778/150‑1 (MQ=255) gCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCa < 2:498270/150‑1 (MQ=255) cATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGcc < 2:625891/150‑1 (MQ=255) tCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGT‑ACCGTCGGTGACGTGctc < 1:70288/64‑3 (MQ=25) tCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGT‑ACCGTCGGTGACGTGctc > 2:70288/1‑62 (MQ=25) gTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGat > 1:798714/1‑150 (MQ=255) tAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGata < 2:71168/150‑1 (MQ=255) gAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAg < 1:459409/150‑1 (MQ=255) aaTCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAgg < 2:798714/150‑1 (MQ=255) ggggTGGAACTTGGTGCGCACCTCACCGTCGGT‑ACGTGCCGATTTCTGGTGCTTGCCATCGGCATCGAGTCCCCACTTGCTCATGGCGTAGACGATGCGTGGCTGCACCGGCTTCTGGCCTTTGCCGATATGCGTCAAGGCACGATCCAt < 2:351671/150‑1 (MQ=255) aCTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGa < 1:409513/137‑1 (MQ=255) aCTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGa > 2:409513/1‑137 (MQ=255) ttGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAGGGCACGATCCATGATCACGTAc > 2:469750/1‑150 (MQ=255) ccGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATg < 1:796355/150‑1 (MQ=255) tcggCACGT‑ACCGTCGGTGACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATgg > 1:187588/4‑150 (MQ=255) gTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGTAGtt < 1:89140/150‑1 (MQ=255) gTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGTAGtt > 2:26028/1‑150 (MQ=255) cGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGTAGTTGAGGt > 1:503817/1‑150 (MQ=255) aCGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGTAGTTGAGGTAg < 1:26028/150‑1 (MQ=255) | GGCGAACGACTTCGGATCATCCGGCGCACCCCAGTTGCCCTGGCCATCGACCAGGGTATAGCGGTAGCTGAACGGCTGCGCCATCAGCACCATGGCCTCGTAGCAGGCCGAATCGCCATGGGGATGGAACTTGCCGAGCACGTCACCGACGGT‑ACGTGCCGACTTCTTGTGCTTGGCATCGGCATCGAGCCCCAACTCGCTCATGGCGTAGACGATGCGTCGCTGCACCGGCTTCAGGCCGTCGCCGATATGCGGCAAGGCACGATCCATGATCACGTACATGGAGTAGTTGAGGTAG > NC_002947/5585414‑5585711 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |