Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,618,370 A→C 8.3% L111L (CTT→CTG)  wzy ← O‑antigen polymerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,618,3700AC 8.3% 132.1 / 4.9 48L111L (CTT→CTG) wzyO‑antigen polymerase
Reads supporting (aligned to +/- strand):  ref base A (20/24);  new base C (2/2);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01

AGGATGGGGTGGGATATCTCGCCGATACCCGCCAGCCTGAACAGCAAAGGCGCACGCTGCACCCCATAGAACTTGATGATGGAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGAGCTCGCGCATGGGCTCCTCGGCGGGCGACCATGCCAGGCTCAGCCCGCTCCAGGCCAACAGCAGCAGCACACTCCCCCACAGCGCCGGTT  >  NC_002947/5618221‑5618518
                                                                                                                                                     |                                                                                                                                                    
aGGATGGGGTGGGATATCTCGCCGATACCCGCCAGCCTGAACAGCAAAGGCGCACGCTGCACCCCATAGAACTTGATGATGGAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCa                                                                                                                                                      >  1:37737/1‑150 (MQ=255)
      gggTGGGATATCTCGCCGATACCCGCCAGCCTGAACAGCAAAGGCGCACGCTGCACCCCATAGAACTTGATGATGGAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTgg                                                                                                                                                <  2:814443/150‑1 (MQ=255)
        gTGGGATATCTCGCCGATACCCGCCAGCCTGAACAGCAAAGGCGCACGCTGCACCCCATAGAACTTGATGATGGAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGc                                                                                                                                              <  1:523180/150‑1 (MQ=255)
                cctcGCCGATACCCGCCAGCCTGAACAGCAAAGGCGCACGCTGCACCCCATAGAACTTGATGATGGAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCg                                                                                                                                      <  2:588225/149‑1 (MQ=255)
                  tcGCCGATACCCGCCAGCCTGAACAGCAAAGGCGCACGCTGCACCCCATAGAACTTGATGATGGAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGa                                                                                                                                    >  1:423734/1‑150 (MQ=255)
                       gATACCCGCCAGCCTGAACAGCAAAGGCGCACGCTGCACCCCATAGAACTTGATGATGGAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGc                                                                                                                               <  1:697366/150‑1 (MQ=255)
                             cGCCAGCCTGAACAGCAAAGGCGCACGCTGCACCCCATAGAACTTGATGATGGAGACCAACGCAGCGATGGCCAGAAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGTCAACAGc                                                                                                                                       <  1:201770/136‑1 (MQ=255)
                             cGCCAGCCTGAACAGCAAAGGCGCACGCTGCACCCCATAGAACTTGATGATGGAGACCAACGCAGCGATGGCCAGAAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGTCAACAGc                                                                                                                                       >  2:201770/1‑136 (MQ=255)
                              gCCAGCCTGAACAGCAAAGGCGCACGCTGCACCCCATAGAACTTGATGATGGAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAAc                                                                                                                                 >  2:587103/1‑141 (MQ=255)
                              gCCAGCCTGAACAGCAAAGGCGCACGCTGCACCCCATAGAACTTGATGATGGAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAAc                                                                                                                                 <  1:587103/141‑1 (MQ=255)
                                       aaCAGCAAAGGCGCACGCTGCACCCCATAGAACTTGATGATGGAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAgg                                                                                                               <  2:37737/150‑1 (MQ=255)
                                           gCAAAGGCGCACGCTGCACCCCATAGAACTTGATGATGGAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCg                                                                                                                                      <  2:592298/123‑1 (MQ=255)
                                           gCAAAGGCGCACGCTGCACCCCATAGAACTTGATGATGGAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCg                                                                                                                                      >  1:592298/1‑123 (MQ=255)
                                            cAAAGGCGCACGCTGCACCCCATAGAACTTGATGATGGAGACCAACGCAGCGATGGCCAACAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTgg                                                                                                                                                <  2:786570/112‑1 (MQ=255)
                                            cAAAGGCGCACGCTGCACCCCATAGAACTTGATGATGGAGACCAACGCAGCGATGGCCAACAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTgg                                                                                                                                                >  1:786570/1‑112 (MQ=255)
                                                    cACGCTGCACCCCATAGAACTTGATGATGGAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAgc                                                                                                  >  1:748236/1‑150 (MQ=255)
                                                          gCACCCCATAGAACTTGATGATGGAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGa                                                                                            <  2:500950/150‑1 (MQ=255)
                                                           cACCCCATAGAACTTGATGATGGAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGAg                                                                                           >  2:576784/1‑150 (MQ=255)
                                                              cccATAGAACTTGATGATGGAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGAGCTc                                                                                        >  2:718295/1‑150 (MQ=255)
                                                                cATAGAACTTGATGATGGAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGAGCTcgc                                                                                      >  1:587199/1‑150 (MQ=255)
                                                                     aaCTTGATGATGGAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGAGCTCGCGCATg                                                                                 <  1:268702/150‑1 (MQ=255)
                                                                       cTTGATGATGGAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGACAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTCAGCTCGCGCATggg                                                                               <  2:86991/150‑1 (MQ=255)
                                                                        ttGATGATGGAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGAGCTCGCGCATGGGc                                                                              >  1:552516/1‑150 (MQ=255)
                                                                            tgatgGAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCtt                                                                                              >  1:210333/1‑130 (MQ=255)
                                                                            tgatgGAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCtt                                                                                              <  2:210333/130‑1 (MQ=255)
                                                                              atgGAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGAGCTCGCGCATGGGCTCCTcg                                                                        <  1:243526/150‑1 (MQ=255)
                                                                                ggAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGAGCTCGCGCATGGGCTCCTcggc                                                                      >  2:733870/1‑150 (MQ=255)
                                                                                          gCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATACCTTAGGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGAGCTCGCGCATGGGCTCCTCGGCGGGCGACCAt                                                            <  1:437772/150‑1 (MQ=255)
                                                                                              cGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGAGCTCGCGCATGGGCTCCTCGGCGGGCGACCATGCCa                                                        <  1:445790/150‑1 (MQ=255)
                                                                                               gATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGAGCTCGCGCATgg                                                                                <  2:389783/125‑1 (MQ=255)
                                                                                               gATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGAGCTCGCGCATgg                                                                                >  1:389783/1‑125 (MQ=255)
                                                                                                      agcagcGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGAGCTCGCGCATGGGCTCCTCGGCGGGCGACCATGCCAGGCTCAGc                                                <  1:718295/150‑1 (MQ=255)
                                                                                                               cTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGAGCTCGCGCATGGGCTCCTCGGCGGGCGACCATGCCAGGCTCAGCCCGCTCCAg                                       <  1:733870/150‑1 (MQ=255)
                                                                                                                    aaGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGAGCTCGCGCATGGGCTCCTCGGCGGGCGACCATGCCAGGCTCAGCCCGCTCCAGGCCaa                                  >  2:406491/1‑150 (MQ=255)
                                                                                                                      gcagcaATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCcagcag                                                                                                                         <  1:14155/61‑1 (MQ=255)
                                                                                                                      gcagcaATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCcagcag                                                                                                                         >  2:14155/1‑61 (MQ=255)
                                                                                                                       cagcaATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGAGCTCGCGCATGGGCTCCTCGGCGGGCGACCATGCCAGGCTCAGCCCGCTCCAGGCCAAcag                               <  2:423734/150‑1 (MQ=255)
                                                                                                                         gcaATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGAGCTCGCGCATGGGCTCCTCGGCGGGCGACCATGCCAGGCTCAGCCCGCTCCAGGCCAAcagca                             <  1:576784/150‑1 (MQ=255)
                                                                                                                          caATAACTGACGAATGAGGGCTTGGCCAAGATGGGCCAACAGCGGAAACGCCAGCAGGACCAACAGGATGTAGCGCAAGCGATTGAGCTCGCGCATGGGCTACAAGGCGGGCGACCCTGCCAGGATCAGCCCGCTCCAGGCCAAcagccg                            >  2:601645/1‑148 (MQ=255)
                                                                                                                              aaCTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGAGCTCGCGCATGGGCTCCTCGGCGGGCGACCATGCCAGGCTCAGCCCGCTCCAGGCCAACagcagcagc                        <  2:748236/150‑1 (MQ=255)
                                                                                                                                           gggCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGAGCTCGCGCATGGGCTCCTCGGCGGGCGACCATGCCAGGCTCAGCCCGCTCCAGGCCAACAGCAGCAGCACACTCCCCcaca           >  2:770231/1‑150 (MQ=255)
                                                                                                                                           gggCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGAGCTCGCGCATGGGCTCCTCGGCGGGCGACCATGCCAGGCTCAGCCCGCTCCAGGCCAACAGCAGCAGCACACTCCCCcaca           >  2:770277/1‑150 (MQ=255)
                                                                                                                                             tgttGGCCCAGCTTGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGa                                                                                            <  2:518122/65‑1 (MQ=38)
                                                                                                                                             tgttGGCCCAGCTTGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGa                                                                                            >  1:518121/3‑67 (MQ=38)
                                                                                                                                             tgttGGCCCAGCTTGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGa                                                                                            <  2:518121/65‑1 (MQ=38)
                                                                                                                                             tgttGGCCCAGCTTGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGa                                                                                            >  1:518122/3‑67 (MQ=38)
                                                                                                                                                   ccAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGAGCTCGCGCATGGGCTCCTCGGCGGGCGACCATGCCAGGCTCAGCCCGCTCCAGGCCAACAGCAGCAGCACACTCCCCCACAGCGCCGGt   >  1:209199/1‑150 (MQ=255)
                                                                                                                                                    cAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGAGCTCGCGCATGGGCTCCTCGGCGGGCGACCATGCCAGGCTCAGCCCGCTCCAGGCCAACAGCAGCAGCACACTCCCCCACAGCGCCGGtt  <  2:209199/150‑1 (MQ=255)
                                                                                                                                                    cAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGAGCTCGCGCATGGGCTCCTCGGCGGGCGACCATGCCAGGCTCAGCCCGCTCCAGGCCAACAGCAGCAGCACACTCCCCCACAGCGCCGGtt  >  1:840272/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AGGATGGGGTGGGATATCTCGCCGATACCCGCCAGCCTGAACAGCAAAGGCGCACGCTGCACCCCATAGAACTTGATGATGGAGACCAACGCAGCGATGGCCAGCAGCGCGCTGCCAAGCAGCAATAACTGACGAATGCGGGCTTGGCCAAGCTGGGCCAACAGCGGAAACGCCAGCAGGAACACCAGGATGTAGAGCAAGCGCTTGAGCTCGCGCATGGGCTCCTCGGCGGGCGACCATGCCAGGCTCAGCCCGCTCCAGGCCAACAGCAGCAGCACACTCCCCCACAGCGCCGGTT  >  NC_002947/5618221‑5618518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑