Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,631,164 G→A 8.9% G215S (GGC→AGC)  putA → proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,631,1640GA 8.9% 121.2 / 2.6 45G215S (GGC→AGC) putAproline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (19/22);  new base A (2/2);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

GCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATGGGTGAGCAGTTCGTCACCGGCGAGACCATTGCC  >  NC_002947/5631015‑5631313
                                                                                                                                                     |                                                                                                                                                     
gCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCg                                                                                                                                                       <  1:134899/150‑1 (MQ=255)
 ccTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCgg                                                                                                                                                      >  1:683549/1‑150 (MQ=255)
  cTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTACTGCTGACCGGc                                                                                                                                                     >  2:62137/1‑150 (MQ=255)
   tGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCa                                                                                                                                                    >  1:653960/1‑150 (MQ=255)
       cGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCt                                                                                                                                                <  2:683549/150‑1 (MQ=255)
        gAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTg                                                                                                                                               <  2:447798/150‑1 (MQ=255)
             cgtgctgcGTATCCCTGACAAAGGTACCCGTGCTGCGATGCACAGAGACCCCATCAACTGCTGCGGCGAGCTGCCGCACCTGGGCAACCGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGTTGCTGCTGACCAGCTTGCCGGTCAg                                                                                                                                          <  1:316779/148‑1 (MQ=255)
              ctgctgCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGc                                                                                                                                         <  1:267514/150‑1 (MQ=255)
              ctgctgCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGc                                                                                                                                         >  2:551024/1‑150 (MQ=255)
               tgctgcGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCCCCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCa                                                                                                                                        <  2:205970/150‑1 (MQ=255)
                   gcGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCa                                                                                                                                    <  1:395998/150‑1 (MQ=255)
                          cccGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGaa                                                                                                                             >  2:423985/1‑150 (MQ=255)
                           ccGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGaaa                                                                                                                            >  1:235906/1‑150 (MQ=255)
                           ccGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGaaa                                                                                                                            >  1:464980/1‑150 (MQ=255)
                                     accccGTGCTGCGCTAATCCACGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCAc                                                                                                                  <  1:247752/148‑1 (MQ=255)
                                      aCCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAATCTGGTCAGCACCCATAACGAAACCGGCCTCAcc                                                                                                                 >  2:267356/1‑150 (MQ=255)
                                        ccGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCAcctc                                                                                                               <  2:709919/150‑1 (MQ=255)
                                                       ccGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGcat                                                                                                <  2:653960/150‑1 (MQ=255)
                                                           gACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCatcatc                                                                                            <  2:622352/150‑1 (MQ=255)
                                                           gACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCatcatc                                                                                            >  2:670836/1‑150 (MQ=255)
                                                            aCAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCAGCTTGCCGGTCAGCagca                                                                                                                                     >  2:695202/1‑105 (MQ=38)
                                                            aCAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCAGCTTGCCGGTCAGCagca                                                                                                                                     <  1:695202/108‑4 (MQ=38)
                                                                 aTCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAag                                                                                      >  1:685360/1‑150 (MQ=255)
                                                                  tCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATAATCGGCAaga                                                                                     <  2:464980/150‑1 (MQ=255)
                                                                      tACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAgcgg                                                                                 >  1:730338/1‑150 (MQ=255)
                                                                       aCCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAgcggc                                                                                <  1:423985/150‑1 (MQ=255)
                                                                       aCCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAgcggc                                                                                <  1:659729/150‑1 (MQ=255)
                                                                                tGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCgatg                                                                       >  1:464232/1‑150 (MQ=255)
                                                                                 ggCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTACTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCgatga                                                                      <  1:62137/150‑1 (MQ=255)
                                                                                  gCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCgatgat                                                                     >  1:128552/1‑150 (MQ=255)
                                                                                      ccGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGc                                                                 <  1:505205/150‑1 (MQ=255)
                                                                                                   aCAGCCCGTCACTGTTCGTCAACGCCCCCACCTGGGGGCTGCTGCTGCCCGGCAAGCTGCTCATCACCCATAACGAAACCCGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACa                                                    <  2:455534/150‑1 (MQ=255)
                                                                                                            cACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATgc                                           <  2:464232/150‑1 (MQ=255)
                                                                                                             aCTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATgcg                                          >  1:91892/1‑150 (MQ=255)
                                                                                                                cttCGTCAACGCCGCCACCTGGGCGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCt                                       <  1:654109/149‑1 (MQ=255)
                                                                                                                  tCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGa                                     <  2:685360/150‑1 (MQ=255)
                                                                                                                     tCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATgg                                  >  1:704168/1‑150 (MQ=255)
                                                                                                                       aaCGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGc                                                                 <  2:669460/117‑1 (MQ=255)
                                                                                                                       aaCGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGc                                                                 >  1:669460/1‑117 (MQ=255)
                                                                                                                                aCCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATGGGTGAGCAGTTc                       <  2:128552/150‑1 (MQ=255)
                                                                                                                                          tgctgctgACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCACGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATGGGTGAGCAGTTCGTCACCg                <  1:60622/147‑1 (MQ=255)
                                                                                                                                          tgctgctgACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATGGGTGAGCAGTTCGTCACCg                >  2:60622/1‑147 (MQ=255)
                                                                                                                                            gtgctgACCAGCTTGCCGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATGGGTGAGCAGTTCGTCACCGGCgag           >  1:171570/2‑150 (MQ=255)
                                                                                                                                             tgctgACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATGGGTGAGCAGTTCGTCACCGGCgaga          <  1:551024/150‑1 (MQ=255)
                                                                                                                                                tgACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATGGGTGAGCAGTTCGTCACCGGCGAGACCa       >  1:725516/1‑150 (MQ=255)
                                                                                                                                                tgACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATGGGTGAGCAGTTCGTCACCGGCGAGACCa       >  1:613906/1‑150 (MQ=255)
                                                                                                                                                    cGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATGGGTGAGCAGTTCGTCACCGGCGAGACCATTGc   >  2:731794/1‑150 (MQ=255)
                                                                                                                                                     ggCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATGGGTGAGCAGTTCGTCACCGGCGAGACCATTGcc  <  2:730338/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCTGGCCGAAGCCCTGCTGCGTATCCCCGACAAAGGTACCCGTGATGCGCTGATCCGCGACAAGATCAGTACCGGCAACTGGCAGCCGCACCTGGGCAACAGCCCGTCACTGTTCGTCAACGCCGCCACCTGGGGGCTGCTGCTGACCGGCAAGCTGGTCAGCACCCATAACGAAACCGGCCTCACCTCCTCGCTCACCCGCATCATCGGCAAGAGCGGCGAGCCGATGATCCGCAAGGGCGTCGACATGGCCATGCGCCTGATGGGTGAGCAGTTCGTCACCGGCGAGACCATTGCC  >  NC_002947/5631015‑5631313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑