Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,660,233	G→T	18.8%	intergenic (+35/‑194)	metK → / → ligB	methionine adenosyltransferase/DNA ligase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,660,233	0	G	T	18.8%	68.3 / 8.5	32	intergenic (+35/‑194)	metK/ligB	methionine adenosyltransferase/DNA ligase B
Reads supporting (aligned to +/- strand):  ref base G (16/10);  new base T (3/3);  total (19/13)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

GCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGCCCGC  >  NC_002947/5660095‑5660374                                                                                                                                           |                                                                                                                                              gCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTGACCACCTTCACCTGGGAGCGCACCGACCGCGCGCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGACAGCCCCTGCCGAGGGATCGGCAgg                                                                                                                                    >  1:391195/1‑150 (MQ=255)   cGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGATCGCTCGGCAgggg                                                                                                                                  <  2:589643/150‑1 (MQ=255)        tCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCtttt                                                                                                                             >  1:672404/1‑150 (MQ=255)                agaagaCTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCt                                                                                                                     <  1:386251/150‑1 (MQ=255)                       tGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACgg                                                                                                              >  2:647716/1‑150 (MQ=255)                                 gacACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCAt                                                                                                     >  1:350309/1‑149 (MQ=255)                                 gacACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCAt                                                                                                     <  2:350309/149‑1 (MQ=255)                                       ttcaccaccttcaccTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCg                                                                                                                                             >  2:585975/1‑103 (MQ=255)                                       ttcaccaccttcaccTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCg                                                                                                                                             <  1:585975/103‑1 (MQ=255)                                         caccaccttcaccTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCAAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTcc                                                                                            >  2:43729/1‑149 (MQ=255)                                           ccaccttcaccTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGa                                                                                          >  1:788270/1‑150 (MQ=255)                                           ccaccttcaccTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCACGCTCATTAAGCGCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGa                                                                                          >  1:787256/1‑150 (MQ=255)                                               cttcaccTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGa                                                                                      >  1:136667/1‑150 (MQ=255)                                                ttcaccTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAg                                                                                     <  2:788270/150‑1 (MQ=255)                                                            cgcACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGt                                                                         <  1:418791/150‑1 (MQ=255)                                                             gcACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGtt                                                                        >  2:699364/1‑150 (MQ=255)                                                                                gTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCGTTTTTGTGCCTGTTACGGCATTTTCATCGCCTTTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCGGTCTCGGGCt                                                     <  2:391195/150‑1 (MQ=255)                                                                                        aCGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGTCg                                             >  2:791341/1‑150 (MQ=255)                                                                                                  ccTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCg                                   >  1:795701/1‑150 (MQ=255)                                                                                                   cTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCtt                                                                    >  1:842746/1‑116 (MQ=255)                                                                                                   cTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCtt                                                                    <  2:842746/116‑1 (MQ=255)                                                                                                           ttCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCAc                          <  1:50559/150‑1 (MQ=255)                                                                                                                  aGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACaa                   <  2:672404/150‑1 (MQ=255)                                                                                                                                  cccTGCCGATCGCTCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCtt                                                    >  2:191932/1‑101 (MQ=255)                                                                                                                                  cccTGCCGATCGCTCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCtt                                                    <  1:191932/101‑1 (MQ=255)                                                                                                                                  cccTGCCGATCGCTCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCAccgcccg   >  1:845781/1‑150 (MQ=255)                                                                                                                                   ccTGCCGATCGCTCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCa                           >  2:640923/1‑125 (MQ=255)                                                                                                                                   ccTGCCGATCGCTCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCa                           <  1:640923/125‑1 (MQ=255)                                                                                                                                   ccTGCCGAGCGATCGGCAGGGGCTTTTTTTTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCACGGGCGTGCGGACGCGCACCAGCGAAATATCACCTGACCACTGCACCCAccgcccgc  >  2:626751/1‑150 (MQ=255)                                                                                                                                   ccTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCCCTCTTGGTGACGAGCACCAGCTAAATATCACCTGACAACTACCAGCAccgccccc  >  2:626788/1‑148 (MQ=255)                                                                                                                                    cTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCa                                      >  2:595717/1‑113 (MQ=255)                                                                                                                                    cTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCa                                      <  1:595717/113‑1 (MQ=255)                                                                                                                                           |                                                                                                                                              GCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGCCCGC  >  NC_002947/5660095‑5660374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑