Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,660,236 A→C 19.4% intergenic (+38/‑191) metK → / → ligB methionine adenosyltransferase/DNA ligase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,660,2360AC19.4% 50.2 / 12.7 31intergenic (+38/‑191)metK/ligBmethionine adenosyltransferase/DNA ligase B
Reads supporting (aligned to +/- strand):  ref base A (16/9);  new base C (3/3);  total (19/12)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.71e-01

GCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGCCCGCCCCGCCACCC  >  NC_002947/5660095‑5660384
                                                                                                                                             |                                                                                                                                                    
gCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTGACCACCTTCACCTGGGAGCGCACCGACCGCGCGCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGACAGCCCCTGCCGAGGGATCGGCAgg                                                                                                                                              >  1:391195/1‑150 (MQ=255)
  cGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGATCGCTCGGCAgggg                                                                                                                                            <  2:589643/150‑1 (MQ=255)
       tCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCtttt                                                                                                                                       >  1:672404/1‑150 (MQ=255)
               agaagaCTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCt                                                                                                                               <  1:386251/150‑1 (MQ=255)
                      tGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACgg                                                                                                                        >  2:647716/1‑150 (MQ=255)
                                gacACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCAt                                                                                                               >  1:350309/1‑149 (MQ=255)
                                gacACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCAt                                                                                                               <  2:350309/149‑1 (MQ=255)
                                        caccaccttcaccTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCAAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTcc                                                                                                      >  2:43729/1‑149 (MQ=255)
                                          ccaccttcaccTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGa                                                                                                    >  1:788270/1‑150 (MQ=255)
                                          ccaccttcaccTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCACGCTCATTAAGCGCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGa                                                                                                    >  1:787256/1‑150 (MQ=255)
                                              cttcaccTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGa                                                                                                >  1:136667/1‑150 (MQ=255)
                                               ttcaccTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAg                                                                                               <  2:788270/150‑1 (MQ=255)
                                                           cgcACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGt                                                                                   <  1:418791/150‑1 (MQ=255)
                                                            gcACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGtt                                                                                  >  2:699364/1‑150 (MQ=255)
                                                                               gTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCGTTTTTGTGCCTGTTACGGCATTTTCATCGCCTTTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCGGTCTCGGGCt                                                               <  2:391195/150‑1 (MQ=255)
                                                                                       aCGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGTCg                                                       >  2:791341/1‑150 (MQ=255)
                                                                                                 ccTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCg                                             >  1:795701/1‑150 (MQ=255)
                                                                                                  cTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCtt                                                                              >  1:842746/1‑116 (MQ=255)
                                                                                                  cTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCtt                                                                              <  2:842746/116‑1 (MQ=255)
                                                                                                          ttCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCAc                                    <  1:50559/150‑1 (MQ=255)
                                                                                                                 aGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACaa                             <  2:672404/150‑1 (MQ=255)
                                                                                                                                 cccTGCCGATCGCTCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCtt                                                              <  1:191932/101‑1 (MQ=255)
                                                                                                                                 cccTGCCGATCGCTCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCtt                                                              >  2:191932/1‑101 (MQ=255)
                                                                                                                                 cccTGCCGATCGCTCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCAccgcccg             >  1:845781/1‑150 (MQ=255)
                                                                                                                                  ccTGCCGATCGCTCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCa                                     >  2:640923/1‑125 (MQ=255)
                                                                                                                                  ccTGCCGATCGCTCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCa                                     <  1:640923/125‑1 (MQ=255)
                                                                                                                                  ccTGCCGAGCGATCGGCAGGGGCTTTTTTTTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCACGGGCGTGCGGACGCGCACCAGCGAAATATCACCTGACCACTGCACCCAccgcccgc            >  2:626751/1‑150 (MQ=255)
                                                                                                                                  ccTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCCCTCTTGGTGACGAGCACCAGCTAAATATCACCTGACAACTACCAGCAccgccccc            >  2:626788/1‑148 (MQ=255)
                                                                                                                                   cTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCa                                                >  2:595717/1‑113 (MQ=255)
                                                                                                                                   cTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCa                                                <  1:595717/113‑1 (MQ=255)
                                                                                                                                            gATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGATGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGCCCGCCCTGCCAccc  >  1:650921/1‑150 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
GCCGTACTCCGCAGCAGAAGACTGTCGGCGACGACACCTTCACCACCTTCACCTGGGAGCGCACCGACCGCGCCCAGTCGTTGCGTGACGCTGCCGGCCTGTAAGCTTCCTGCAGCGCCAGACGAAAGCCCCTGCCGAGCGATCGGCAGGGGCTTTTTTGTGCCTGTTACGGCATTTTCATCGCCCGTCAGATCGAGCGCCGCGCTCGTTTCTTGCTCCGTCTCGGGCTTGGAGACGAGCACCAGCGAAATATCACCTGACAACTGCACGCACCGCCCGCCCCGCCACCC  >  NC_002947/5660095‑5660384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑