Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,683,074 T→A 11.1% D1292V (GAC→GTC)  PP_4988 ← signal transduction histidine kinase CheA/CheY‑like receiver

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,683,0740TA11.1% 98.4 / 3.8 36D1292V (GAC→GTC) PP_4988signal transduction histidine kinase CheA/CheY‑like receiver
Reads supporting (aligned to +/- strand):  ref base T (16/16);  new base A (2/2);  total (18/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01

ACCCAGTTGCTTGACCTCCTCGTGCACCACATCCATGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGACGATATCGGCGCCCTCATGCAGCAAATTCAGGTGAATGGTGCCCTGCTCCGG  >  NC_002947/5682935‑5683204
                                                                                                                                           |                                                                                                                                  
aCCCAGTTGCTTGACCTCCTCGTGCACCACATCCATGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCTGGACCCGCAGGc                                                                                                                           <  2:759079/149‑1 (MQ=255)
aCCCAGTTGCTTGACCTCCTCGTGCACCACATCCATGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCTGGACCCGCAGGc                                                                                                                           >  1:759079/1‑149 (MQ=255)
 cccAGTTGCTTGACCTCCTCGTGCACCACATCCATGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCca                                                                                                                                 >  1:197163/1‑142 (MQ=255)
 cccAGTTGCTTGACCTCCTCGTGCACCACATCCATGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCca                                                                                                                                 <  2:197163/142‑1 (MQ=255)
 cccAGTTGCTTGACCTCCTCGTGCACCACATCCATGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCcg                                                                                                                         >  1:296060/1‑150 (MQ=255)
       tGCTTGACCTCCTCGTGCACCACATCCATGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGAATGAACAGCAGGTGCTCTTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTg                                                                                                                   >  1:406366/1‑150 (MQ=255)
          ttGACCTCCTCGTGCACCACATCCATGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATg                                                                                                                >  2:508781/1‑150 (MQ=255)
                 cctcGTGCACCACATCCATGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTgc                                                                                                         <  1:486950/150‑1 (MQ=255)
                  ctcGTGCACCACATCCATGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTgcg                                                                                                        >  1:807833/1‑150 (MQ=255)
                   tcGTGCACCACATCCATGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTgcgc                                                                                                       <  2:508768/150‑1 (MQ=255)
                                   tGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAggg                                                                                       <  2:171247/150‑1 (MQ=255)
                                      ccAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCa                                                                                    >  1:276721/1‑150 (MQ=255)
                                               gcCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGGACGGGACCGCCTCCAGGGGCACGCCGGCAc                                                                           <  1:451321/150‑1 (MQ=255)
                                                cccGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCTGGACCCGCAGGcgc                                                                                                                         >  1:602128/1‑101 (MQ=255)
                                                cccGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCTGGACCCGCAGGcgc                                                                                                                         <  2:602128/103‑3 (MQ=255)
                                                cccGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGTGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCAcc                                                                          <  2:303495/150‑1 (MQ=255)
                                                   ggAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACcatc                                                                       <  1:608509/150‑1 (MQ=255)
                                                             gTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGTATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCa                                                                                          >  2:711033/1‑121 (MQ=255)
                                                             gTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGTATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCa                                                                                          <  1:711033/121‑1 (MQ=255)
                                                                    tttCGGCGCTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGa                                                      <  1:145294/150‑1 (MQ=255)
                                                                     ttCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATg                                                                                                                <  1:597963/91‑1 (MQ=255)
                                                                     ttCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATg                                                                                                                >  2:597963/1‑91 (MQ=255)
                                                                     ttCGGCGGCGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGAc                                                     <  1:21126/150‑1 (MQ=255)
                                                                        ggcggTGGAAAAGCCTAGCAGCAGGATGATCTGCGGGATGTCGTGGGCGCGGAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGACGat                                                  >  2:824182/1‑150 (MQ=255)
                                                                            gTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGACGATATCg                                              <  2:276721/150‑1 (MQ=255)
                                                                                aaaaGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGACGATATCGgcgc                                          >  2:613265/1‑150 (MQ=255)
                                                                                           cGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGACGATATCGGCGCCCTCATgcagc                               >  2:653359/1‑150 (MQ=255)
                                                                                             cAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTgcgc                                                                                                       >  2:618300/1‑76 (MQ=255)
                                                                                             cAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTgcgc                                                                                                       <  1:618300/76‑1 (MQ=255)
                                                                                                gATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGACGATATCGGCGCCCTCATccagccgata                          >  2:308888/1‑145 (MQ=255)
                                                                                                                      tCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCatcatc                                                                    >  2:593134/1‑86 (MQ=255)
                                                                                                                      tCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCatcatc                                                                    <  1:593134/86‑1 (MQ=255)
                                                                                                                        aCTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGACGATATCGGCGCCCTCATGCAGCAAATTCAGGTGAATGGTGCCCTGCTCCgg  >  2:622284/1‑150 (MQ=255)
                                                                                                                        aCTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGACGATATCGGCGCCCTCATGCAGCAAAGTCAGGGGAATGGTGCCCTGCTCCgg  <  2:406366/150‑1 (MQ=255)
                                                                                                                                    cTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGACGATATCGGCGCCCTCATGCAGCAAATTCAGGTGAATGGTGCCCTGCTCCg   <  2:278698/137‑1 (MQ=255)
                                                                                                                                    cTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGACGATATCGGCGCCCTCATGCAGCAAATTCAGGTGAATGGTGCCCTGCTCCg   >  1:278698/1‑137 (MQ=255)
                                                                                                                                           |                                                                                                                                  
ACCCAGTTGCTTGACCTCCTCGTGCACCACATCCATGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGACGATATCGGCGCCCTCATGCAGCAAATTCAGGTGAATGGTGCCCTGCTCCGG  >  NC_002947/5682935‑5683204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑