| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,683,077 | A→C | 11.8% | L1291R (CTG→CGG) | PP_4988 ← | signal transduction histidine kinase CheA/CheY‑like receiver |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,683,077 | 0 | A | C | 11.8% | 81.0 / 5.5 | 34 | L1291R (CTG→CGG) | PP_4988 | signal transduction histidine kinase CheA/CheY‑like receiver |
| Reads supporting (aligned to +/- strand): ref base A (15/15); new base C (2/2); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.30e-01 | |||||||||||
ACCCAGTTGCTTGACCTCCTCGTGCACCACATCCATGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGACGATATCGGCGCCCTCATGCAGCAAATTCAGGTGAATGGTGCCCTGCTCCGGCTTGCCGGCGGCCAGGCGCATT > NC_002947/5682935‑5683226 | aCCCAGTTGCTTGACCTCCTCGTGCACCACATCCATGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCTGGACCCGCAGGc < 2:759079/149‑1 (MQ=255)aCCCAGTTGCTTGACCTCCTCGTGCACCACATCCATGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCTGGACCCGCAGGc > 1:759079/1‑149 (MQ=255) cccAGTTGCTTGACCTCCTCGTGCACCACATCCATGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCca > 1:197163/1‑142 (MQ=255) cccAGTTGCTTGACCTCCTCGTGCACCACATCCATGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCca < 2:197163/142‑1 (MQ=255) cccAGTTGCTTGACCTCCTCGTGCACCACATCCATGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCcg > 1:296060/1‑150 (MQ=255) tGCTTGACCTCCTCGTGCACCACATCCATGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGAATGAACAGCAGGTGCTCTTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTg > 1:406366/1‑150 (MQ=255) ttGACCTCCTCGTGCACCACATCCATGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATg > 2:508781/1‑150 (MQ=255) cctcGTGCACCACATCCATGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTgc < 1:486950/150‑1 (MQ=255) ctcGTGCACCACATCCATGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTgcg > 1:807833/1‑150 (MQ=255) tcGTGCACCACATCCATGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTgcgc < 2:508768/150‑1 (MQ=255) tGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAggg < 2:171247/150‑1 (MQ=255) ccAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCa > 1:276721/1‑150 (MQ=255) gcCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGGACGGGACCGCCTCCAGGGGCACGCCGGCAc < 1:451321/150‑1 (MQ=255) cccGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCTGGACCCGCAGGcgc > 1:602128/1‑101 (MQ=255) cccGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCTGGACCCGCAGGcgc < 2:602128/103‑3 (MQ=255) cccGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGTGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCAcc < 2:303495/150‑1 (MQ=255) ggAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACcatc < 1:608509/150‑1 (MQ=255) gTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGTATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCa < 1:711033/121‑1 (MQ=255) gTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGTATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCa > 2:711033/1‑121 (MQ=255) tttCGGCGCTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGa < 1:145294/150‑1 (MQ=255) ttCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATg < 1:597963/91‑1 (MQ=255) ttCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATg > 2:597963/1‑91 (MQ=255) ttCGGCGGCGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGAc < 1:21126/150‑1 (MQ=255) ggcggTGGAAAAGCCTAGCAGCAGGATGATCTGCGGGATGTCGTGGGCGCGGAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGACGat > 2:824182/1‑150 (MQ=255) gTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGACGATATCg < 2:276721/150‑1 (MQ=255) aaaaGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGACGATATCGgcgc > 2:613265/1‑150 (MQ=255) cGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGACGATATCGGCGCCCTCATgcagc > 2:653359/1‑150 (MQ=255) cAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTgcgc > 2:618300/1‑76 (MQ=255) cAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTgcgc < 1:618300/76‑1 (MQ=255) gATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGACGATATCGGCGCCCTCATccagccgata > 2:308888/1‑145 (MQ=255) tCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCatcatc > 2:593134/1‑86 (MQ=255) tCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCatcatc < 1:593134/86‑1 (MQ=255) aCTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGACGATATCGGCGCCCTCATGCAGCAAATTCAGGTGAATGGTGCCCTGCTCCgg > 2:622284/1‑150 (MQ=255) aCTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGACGATATCGGCGCCCTCATGCAGCAAAGTCAGGGGAATGGTGCCCTGCTCCgg < 2:406366/150‑1 (MQ=255) cTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGACGATATCGGCGCCCTCATGCAGCAAATTCAGGTGAATGGTGCCCTGCTCCg > 1:278698/1‑137 (MQ=255) cTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGACGATATCGGCGCCCTCATGCAGCAAATTCAGGTGAATGGTGCCCTGCTCCg < 2:278698/137‑1 (MQ=255) agcagGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGACGATATCGGCGCCCTCATGCAGCAAATTCAGGTGAATGGTGCCCTGCTCCGGCTTGCCGGCGGCCAGGCGCAtt < 1:824182/150‑1 (MQ=255) | ACCCAGTTGCTTGACCTCCTCGTGCACCACATCCATGCCCAGCCCGCGCCCGGAAATCTGGGTGATCTTTTCGGCGGTGGAAAAGCCTGGCCGCAGGATGAACTGCAGGATCTCGTGGTCACTCAACTGCGCCTGCGGGTCCAGCAGGCCGCGCTTGATGGCCTTGCGCCGCACCGCCTCCAGGGGCACGCCGGCACCATCATCCGTCATTTCGATGACGATATCGGCGCCCTCATGCAGCAAATTCAGGTGAATGGTGCCCTGCTCCGGCTTGCCGGCGGCCAGGCGCATT > NC_002947/5682935‑5683226 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |