Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,718,633 G→A 8.9% A426T (GCC→ACC)  PP_5020 → methyl‑accepting chemotaxis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,718,6330GA 8.9% 120.9 / 3.2 45A426T (GCC→ACC) PP_5020methyl‑accepting chemotaxis protein
Reads supporting (aligned to +/- strand):  ref base G (20/21);  new base A (2/2);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

TCGGCGGTGACAGAACAGACCAGCGCCGGGGTCAACAATCAGAAGGTCGAAACCGACCAGGTGGCGACTGCGATGAACCAGATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAGCGCCTGGCCAGCGAAGTGGTCAACTCCAGCGAAGCGATGAACCTGCTCAAGACCGAAAGCGACAAGATCGGCAGCGTA  >  NC_002947/5718489‑5718770
                                                                                                                                                |                                                                                                                                         
tCGGCGGTGACAGAACAGACCAGCGCGGGGGTCAACAATCAGAAGGTCGAAACCGACCAGGTGGCGACTGCGATGAACCAGATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGAc                                                                                                                                      >  2:147757/1‑150 (MQ=255)
        gACAGAACAGACCAGCGCCGGGGTCAACAATCAGAAGGTCGAAACCGACCAGGTGGCGACTGCGATGAACCAGATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGc                                                                                                                              >  2:487818/1‑150 (MQ=255)
                agaCCAGCGCCGGGGTCAACAATCAGAAGGTCGAAACCGACCAGGTGGCGACTGCGATGAACCAGATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAgg                                                                                                                      <  2:170169/150‑1 (MQ=255)
                  aCCAGCGCCGGGGTCAACAATCAGAAGGTCGAAACCCTGTTTGTGGCGACTGCGATGAACCAGATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGt                                                                                                                    <  1:262234/150‑1 (MQ=39)
                     aGCGCCGGGGTCAACAATCAGAAGGTCGAAACCGACCAGGTGGCGACTGCGATGAACCAGATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGatgacatt                                                                                                                 <  2:443863/150‑8 (MQ=255)
                             ggTCAACAATCAGAAGGTCGAAACCGACCAGGTGGCGACTGCGATGAACCAGATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCgtggt                                                                                                         >  1:364233/1‑150 (MQ=255)
                               tCAACAATCAGAAGGTCGAAACCGACCAGGTGGCGACTGCGATGAACCAGATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGtggtgg                                                                                                       >  1:562295/1‑150 (MQ=255)
                               tCAACAATCAGAAGGTCGAAACCGACCAGGTGGCGACTGCGATGAACCAGATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGtggtgg                                                                                                       <  2:627900/150‑1 (MQ=255)
                                 aacaaTCAGAAGGTCGAAACCGACCAGGTGGCGACTGCGATGAACCAGATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGc                                                                                                     >  1:635183/1‑150 (MQ=255)
                                   caaTCAGAAGGTCGAAACCGACCAGGTGGCGACTGCGATGAACCAGATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGa                                                                                                   <  2:756548/150‑1 (MQ=255)
                                         gAAGGTCGAAACCGACCAGGTGGCGACTGCGATGAACCAGATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGt                                                                                             <  1:504665/150‑1 (MQ=255)
                                             gTCGAAACCGACCAGGTGGCGACTGCGATGAACCAGATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGca                                                                                         >  2:75430/1‑150 (MQ=255)
                                                gAAACCGACCAGGTGGCGACTGCGCTGATCCATATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAg                                                                                      >  1:451615/1‑150 (MQ=255)
                                                 aaaCCGACCAGGTGGCGACTGCGATGAACCAGATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGc                                                                                                                                          <  2:449797/97‑1 (MQ=255)
                                                 aaaCCGACCAGGTGGCGACTGCGATGAACCAGATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGc                                                                                                                                          >  1:449797/1‑97 (MQ=255)
                                                 aaaCCGACCAGGTGGCGACTGCGATGAACCAGATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGa                                                                                     <  1:75430/150‑1 (MQ=255)
                                                     cGACCAGGTGGCGACTGCGATGAACCAGATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGa                                                                                 <  1:220456/150‑1 (MQ=255)
                                                          aGGTGGAGACGGCGATGAACCAGATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGACGACCTGATGGCCGACCAGCAGGCCCGCGAGGGTCATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAGCGcc                                                                            <  1:487818/150‑1 (MQ=39)
                                                            gTGGCGACTGCGATGAACCAGATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAGCGCCTg                                                                          >  1:732525/1‑150 (MQ=255)
                                                                    tGCGATGAACCAGATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAgc                                                                               <  2:21909/137‑1 (MQ=255)
                                                                    tGCGATGAACCAGATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAgc                                                                               >  1:21909/1‑137 (MQ=255)
                                                                               aGATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGACCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAGCGCCTGGCCAGCGAAGTGGTCAACt                                                       >  2:456964/1‑150 (MQ=255)
                                                                               aGATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGACCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAGCGCCTGGCCAGCGAAGTGGTCAACt                                                       >  2:456962/1‑150 (MQ=255)
                                                                                    aCTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGACCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAGCGCCTGGCCAGCGAAGTGGTCAACTCCagc                                                  <  1:456964/150‑1 (MQ=255)
                                                                                    aCTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGACCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAGCGCCTGGCCAGCGAAGTGGTCAACTCCagc                                                  <  1:456962/150‑1 (MQ=255)
                                                                                       gCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAGCGCCTGGCCAGCGAAGTGGTCAACTCCagcgaa                                               <  2:451615/150‑1 (MQ=255)
                                                                                        ccACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAgc                                                                               >  1:740794/1‑117 (MQ=255)
                                                                                        ccACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAgc                                                                               <  2:740794/117‑1 (MQ=255)
                                                                                        ccACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAGCGCCTGGCAAGCCAAGGGGTCAATGCCagcgaat                                              <  2:635183/150‑2 (MQ=255)
                                                                                                 aCGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAGCGCCTGGCCAGCGAAGTGGTCAACTCCagcgaagc                                             <  2:819797/142‑1 (MQ=255)
                                                                                                 aCGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAGCGCCTGGCCAGCGAAGTGGTCAACTCCagcgaagc                                             >  1:819797/1‑142 (MQ=255)
                                                                                                 aCGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGACCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAGCGCCTGGCCAGCGAAGTGGTCAAc                                                        >  2:288931/1‑131 (MQ=255)
                                                                                                 aCGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGACCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAGCGCCTGGCCAGCGAAGTGGTCAAc                                                        <  1:288931/131‑1 (MQ=255)
                                                                                                         gCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAGCGCCTGGCCCGGGAAGTGGTCAACTCCAGCGAAGCGATGAACCTGCTCAAg                             >  1:7820/1‑150 (MQ=255)
                                                                                                          cACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAgc                                                                               >  2:128807/1‑99 (MQ=255)
                                                                                                          cACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAgc                                                                               <  1:128807/99‑1 (MQ=255)
                                                                                                            ctctaTGCCTAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAGCGCCTGGCCAGCGAAGTGGTCAACTCCAGCGAAGCGATGAACCTGCTCAAGAcc                          <  2:732525/146‑1 (MQ=255)
                                                                                                                 tGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAGCGCCTGGCCAGCGAAGTGGTCAACTCCAGCGAAGCGATGAACCTGCTCAAGACCGAAAg                     <  1:165240/150‑1 (MQ=255)
                                                                                                                           gCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAGCGCCTGGCCAGCGAAGTGGTCAACTCCAGCGAAGCGATGAACCTGCTCAAGACCGAAAGCGACAAGATc           >  1:291114/1‑150 (MQ=255)
                                                                                                                             ttCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAGCGCCTGGCCAGCGAAGTGGTCAACTCCAGCGAAGCGATGAACCTGCTCAAGACCGAAAGCGACAAGATCgg         >  1:197608/1‑150 (MQ=255)
                                                                                                                                   aGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAGCGCCTgg                                                                         >  1:510089/1‑80 (MQ=255)
                                                                                                                                   aGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAGCGCCTgg                                                                         <  2:510089/80‑1 (MQ=255)
                                                                                                                                    gccgccTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAGCGCCTGGCCAGCGAAGTGGTCAACTCCAGCGAAGCGATGAACCTGCTCAAGACCGAAAGCGACAAGATCGGCAGCGTa  >  1:421622/1‑150 (MQ=255)
                                                                                                                                     ccgccTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAGCGCCTGGCCAGCGAAGTGGTCAACTCCAGCGAAGCGATGAACCt                                    >  1:748821/1‑115 (MQ=255)
                                                                                                                                     ccgccTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAGCGCCTGGCCAGCGAAGTGGTCAACTCCAGCGAAGCGATGAACCt                                    <  2:748821/115‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                         
TCGGCGGTGACAGAACAGACCAGCGCCGGGGTCAACAATCAGAAGGTCGAAACCGACCAGGTGGCGACTGCGATGAACCAGATGACTGCCACCGTGCACGAAGTGGCACGCAATGCCGAACAGGCTTCGGAAGCCGCCTTGATGGCCGACCAGCAGGCCCGCGAGGGTGATCGCGTGGTGGGCGAGGCAGTGGCACAGATCGAGCGCCTGGCCAGCGAAGTGGTCAACTCCAGCGAAGCGATGAACCTGCTCAAGACCGAAAGCGACAAGATCGGCAGCGTA  >  NC_002947/5718489‑5718770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑