| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,737,655 | G→C | 30.8% | intergenic (‑154/+48) | hutU ← / ← PP_5034 | urocanate hydratase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,737,655 | 0 | G | C | 30.8% | 31.7 / 20.9 | 26 | intergenic (‑154/+48) | hutU/PP_5034 | urocanate hydratase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (8/10); new base C (4/4); total (12/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01 | |||||||||||
TCCTCTGCGGTCGTCCGCGGGCGGGTATGTCGTGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCC > NC_002947/5737510‑5737799 | tCCTCTGCGGTCGTCCGCGGGCGGGTATGTCGTGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCcgcccgcg > 2:357427/1‑148 (MQ=255) gCGGTCGTCCGCGGGCGGGTATGTCGTGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCCCGCGCGgcgc < 1:357427/150‑1 (MQ=255) tcgtcCGCGGGCGGGTATGTCGTGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGa < 1:534635/150‑1 (MQ=255) cgtcCGCGGGCGGGTATGTCGTGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGAt < 1:361481/150‑1 (MQ=255) cgggcgggTATGTCGTGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTcaca < 1:699589/150‑1 (MQ=255) ggcgggTATGTCGTGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTAGAGATCGAGCGCCGCCCGCGCGGCGCTCGAtctataccc < 1:752739/150‑7 (MQ=39) tCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCCCGCGCGGCGCTCGa > 1:553308/1‑125 (MQ=255) tCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTCCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCCCGCGCGGCGCTCGa < 2:553308/125‑1 (MQ=255) accccTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCCTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAgc < 1:97033/147‑1 (MQ=255) acacCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGTGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAgc > 2:511724/1‑150 (MQ=255) acCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGTGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAgcgc < 1:511724/150‑1 (MQ=255) tttACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCa > 1:621702/1‑150 (MQ=255) taCAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATc > 1:473662/1‑150 (MQ=255) ccAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGcccc < 2:473662/150‑1 (MQ=255) cAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCt > 1:722864/1‑150 (MQ=255) cTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATtcat < 2:722864/150‑1 (MQ=255) tGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATtcatc < 1:608854/150‑1 (MQ=255) aTTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCt < 1:647315/116‑1 (MQ=255) aTTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCt > 2:647315/1‑116 (MQ=255) aTCGAGCGCCGCGCGGGCGGCGCTCCATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCATCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGaa < 1:333867/150‑1 (MQ=255) tCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGaac > 1:260004/1‑150 (MQ=255) gAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGaacaa > 2:322049/1‑150 (MQ=255) gAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGccc < 1:559603/93‑1 (MQ=255) gAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGccc > 2:559603/1‑93 (MQ=255) aGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGACAACACCGCCCCTGCACAACCATCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGaacaac > 2:312372/1‑150 (MQ=39) gcgcCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAAcc > 2:336178/1‑150 (MQ=255) gccgcGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAAcccc > 2:455790/1‑150 (MQ=255) | TCCTCTGCGGTCGTCCGCGGGCGGGTATGTCGTGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAACAACCCC > NC_002947/5737510‑5737799 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |