Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,807,262 A→G 10.8% T599A (ACA→GCA)  mrcA → penicillin‑insensitive transglycosylase/penicillin‑sensitive transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,807,2620AG10.8% 93.3 / 3.3 37T599A (ACA→GCA) mrcApenicillin‑insensitive transglycosylase/penicillin‑sensitive transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (16/17);  new base G (2/2);  total (18/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.30e-01

GCAGGACCTGCCGCGCAACCTGTCGCTGGCCCTGGGCACCGCCACCCTGACCCCGATGGAAATCGCCACCGGCTGGAGCACCTTTGCCAACGGTGGGTACAAGATCACCCCGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGGCCGCCCCCGAGCAGCCAATCAGCACTGCGGCCATGCCAGGCGAGGCACCTTCTGCCTTCAGTCAGGTGGCCGCGGCATCGCAAGCGCCG  >  NC_002947/5807114‑5807411
                                                                                                                                                    |                                                                                                                                                     
gCAGGACCTGCCGCGCAACCTGTCGCTGGCCCTGGGCACCGCCACCCTGACCCCGATGGAAATCGCCACCGGCTGGAGCACCTTTGCCAACGGTGGGTACAAGATCACCCCGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGac                                                                                                                                                      >  1:266819/1‑150 (MQ=255)
        tGCCGCGCAACCTGTCGCTGGCCCTGGGAACCGCCACCCTGACCCCGATGGAAATCGCCACCGGCTGGAGCACCTTTGCCAACGGTGGGTACAAGATCACCCCGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCa                                                                                                                                              <  1:74557/150‑1 (MQ=255)
           cgcgCAACCTGTCGCTGGCCCTGGGCACCGCCACCCTGACCCCGATGGAAATCGCCACCTGCTGGAGCACCTTTGCCAACGGCGGGTACAAGATCACCCCGTACCTGATCGAGCGCATCGAGCGCCGCAGCGGGGAGACACTGTTCACCg                                                                                                                                           >  2:787445/1‑150 (MQ=255)
                  ccTGTCGCTGGCCCTGGGCACCGCCACCCTGACCCCGATGGAAATCGCCACCGGCTGGAGCACCTTTGCCAACGGTGGGTACAAGATCACCCCGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAAccc                                                                                                                                    >  2:513659/1‑150 (MQ=255)
                  ccTGTCGCTGGCCCTGGGCACCGCCACCCTGACCCCGATGGAAATCGAACAAGAATAGCGCACCTTTGCCAACGGTGGGTACAAGATCACCCCGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGACACACTGTTCCCCGCCAAccc                                                                                                                                    >  2:423066/1‑150 (MQ=255)
                    tGTCGCTGGCCCTGGGCACCGCCACCCTGACCCCGATGGAAATCGCCACCGGCTGGAGCACCTTTGCCAACGGTGGGTACAAGATCACCCCGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCgg                                                                                                                                  >  1:380706/1‑150 (MQ=255)
                          tGGCCCTGGGCACCGCCACCCTGACCCCGATGGAAATCGCCACCGGCTGGAGCACCTTTGCCAACGGTGGGTACAAGATCACCCCGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGc                                                                                                                                 >  2:12319/1‑145 (MQ=255)
                          tGGCCCTGGGCACCGCCACCCTGACCCCGATGGAAATCGCCACCGGCTGGAGCACCTTTGCCAACGGTGGGTACAAGATCACCCCGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGc                                                                                                                                 <  1:12319/145‑1 (MQ=255)
                            gCCCTGGGCACCGCCACCCTGACCCCGATGGAAATCGCCACCGGCTGGAGCACCTTTGCCAACGGTGGGTACAAGATCACCCCGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTa                                                                                                                          >  1:373287/1‑150 (MQ=255)
                              ccTGGGCACCGCCACCCTGACCCCGATGGAAATCGCCACCGGCTGGAGCACCTTTGCCAACGGTGGGTACAAGGACTCCCCGTACCTGATCCAGCGCCTCGAGAGCCGCATCTGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTAcc                                                                                                                        >  1:829174/1‑150 (MQ=255)
                               cTGGGCACCGCCACCCTGACCCCGATGGAAATCGCCACCGGCTGGAGCACCTTTGCCAACGGAGGGTACGAGAGGACCCCGGACCTGATCGAGCGCGTCGAGAGGCGCAGCGGCGAGACACTGTTCACCGCAAACCCGGCCCGCGTACCg                                                                                                                       >  1:683344/1‑150 (MQ=255)
                                                          gAAATCGCCACCGGCTGGAGCACCTTTGCCAACGGTGGGTACAAGATCACCCCGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTg                                                                                            <  2:266819/150‑1 (MQ=255)
                                                           aaaTCGCCACCGGCTGGAGCACCTTTGCCAACGGTGGGTACAAGATCACCCCGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTgg                                                                                           >  1:269807/1‑150 (MQ=255)
                                                            aaTCGCCACCGGCTGGAGCACCTTTGCCAACGGTGGGTACAAGATCACCCCGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGgc                                                                                          <  2:373287/150‑1 (MQ=255)
                                                             aTCGCCACCGGCTGGAGCACCTTTGCCAACGGTGGGTACAAGATCACCCCGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGgcc                                                                                         >  1:123601/1‑150 (MQ=255)
                                                               cGCCACCGGCTGGAGCACCTTTGCCAACGGTGGGTACAAGATCACCCCGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCCCGCCTggtctt                                                                                       <  2:380706/150‑5 (MQ=255)
                                                                     cGGCTGGAGCACCTTTGCCAACGGTGGGTACAAGATCACCCCGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGGCCGCCCCCGa                                                                                 <  2:123601/150‑1 (MQ=255)
                                                                         tGGAGCACCTTTGCCAACGGTGGGTACAAGATCACCCCGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGGCCGCCCCCGagcag                                                                             <  2:829174/150‑1 (MQ=255)
                                                                         tGGAGCACCTTTGCCAACGGTGGGTACAAGATCACCCCGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGGCCGCCCCCGagcag                                                                             >  1:818015/1‑150 (MQ=255)
                                                                                             tGGGTACAAGCTCACCCCGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGGCCGCCCCCGAGCAGCCAATCAGCACTGCGGCCAt                                                         <  2:683344/150‑1 (MQ=255)
                                                                                               ggTACAAGATCACCCCGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGGCCGCCCCCGAGCAGCCAATCAGCACTGCGGCCATGc                                                       <  2:14795/150‑1 (MQ=255)
                                                                                                      gATCCCCCCGTACCTAATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGGCCGCCCCCGAGCAGCCAATCAGCACTGCGGCCATGCCAGGCGa                                                <  1:633623/150‑1 (MQ=255)
                                                                                                      gATCACCCCGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGGCCGCCCCCGag                                                                                >  1:59297/1‑118 (MQ=255)
                                                                                                      gATCACCCCGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGGCCGCCCCCGag                                                                                <  2:59297/118‑1 (MQ=255)
                                                                                                      aaTCACCCCGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGGCCGCCCCCGAGCAGCCAATCAGCACTGCGGCCATGCCAGGCGa                                                <  2:205587/149‑1 (MQ=255)
                                                                                                           ccccGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGGCCGCCCCCGAGCAGCCAATCAGCACTGCGGCCATGCCAGGCGAGGCAc                                           >  1:142095/1‑150 (MQ=255)
                                                                                                              cGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGGCCGCCCCCGAGCAGCAAATCAGCACTGCGGCCATGCCAGGCGAGGCACCtt                                        >  2:406397/1‑150 (MQ=255)
                                                                                                                tACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGTCCAAGACCAGGCGGGCCTGGCCGCCCCCGAGCAGCAAATCAGCACTGCGGCCATGCCAGGCGAGGCACCTTCt                                      <  1:406397/150‑1 (MQ=255)
                                                                                                                    tGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGGCCGCCCCCGAGCAGCCAATCAGCACTGCGGCCATGCCAGGCGAGGCACCTTCTGCCt                                  >  1:424240/1‑150 (MQ=255)
                                                                                                                       tCGAGCGCATCGAGAGCCGCAGCGGCGAGGCACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGGCCGCCCCCGAGCAGCCAATCAGCACTGCGGCCATGCCAGGCGAGGCACCTTCTGc                                    >  1:571523/1‑145 (MQ=255)
                                                                                                                       tCGAGCGCATCGAGAGCCGCAGCGGCGAGGCACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGGCCGCCCCCGAGCAGCCAATCAGCACTGCGGCCATGCCAGGCGAGGCACCTTCTGc                                    <  2:571523/145‑1 (MQ=255)
                                                                                                                       tCGAGCGCATCGAGAGCCGCAGCGGCGAGGCACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGGCCGCCCCCGAGCAGCCAATCAGCACTGCGGCCATGCCAGGCGAGGCACCTTCTGc                                    <  2:571829/145‑1 (MQ=255)
                                                                                                                       tCGAGCGCATCGAGAGCCGCAGCGGCGAGGCACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGGCCGCCCCCGAGCAGCCAATCAGCACTGCGGCCATGCCAGGCGAGGCACCTTCTGc                                    >  1:571829/1‑145 (MQ=255)
                                                                                                                         gAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGGCCGCCCCCGAGCAGCCAATCAGCACTGCGGCCATGCCAGGCGAGGCACCTTCTGCCTtcagt                             <  2:818015/150‑1 (MQ=255)
                                                                                                                               aTCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGGCCGCCCCCGAGCAGCCAATCAGCACTGCGGCCATGCCAGGCGAGGCACCTTCTGCCTTCAGTCAGGTg                       <  2:488382/150‑1 (MQ=255)
                                                                                                                                tCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGGCCGCCCCCGAGCAGCCAATCAGCACTGCGGCCATGCCAGGCGAGGCACCTTCTGCCTTCCGTCAGGTgg                      >  1:766438/1‑150 (MQ=255)
                                                                                                                                          cAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGGCCGCCCCCGAGCAGCCAATCAGCACTGCGGCCATGCCAGGCGAGGCACCTTCTGCCTTCAGTCAGGTGGCCGCGGCATc            <  2:769134/150‑1 (MQ=255)
                                                                                                                                              ggcgAGACACTGTTCACCGCCAACCCTGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGGCCGCCCCCGAGCAGCCAATCAGCACTGCGGCCATGCCAGGCGAGGCACCTTCTGCCTTCAGTCAGGTGGCTGCGGCATCGCaa        <  2:212902/150‑1 (MQ=255)
                                                                                                                                                    acacTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGGCCGCCCCCGAGCAGCCAATCAGCACTGCGGCCATGCCAGGCGAGGCACCTTCTGCCTTCAGTCAGGTGGCCGCGGCATCGCAAGCGCCg  >  2:796386/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCAGGACCTGCCGCGCAACCTGTCGCTGGCCCTGGGCACCGCCACCCTGACCCCGATGGAAATCGCCACCGGCTGGAGCACCTTTGCCAACGGTGGGTACAAGATCACCCCGTACCTGATCGAGCGCATCGAGAGCCGCAGCGGCGAGACACTGTTCACCGCCAACCCGGCCCGCGTACCGCAAGGTGCCCAAGACCAGGCGGGCCTGGCCGCCCCCGAGCAGCCAATCAGCACTGCGGCCATGCCAGGCGAGGCACCTTCTGCCTTCAGTCAGGTGGCCGCGGCATCGCAAGCGCCG  >  NC_002947/5807114‑5807411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑