Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,824,905 A→C 7.8% Y208* (TAT→TAG)  trmB ← tRNA (guanosine(46)‑N7)‑methyltransferase TrmB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,824,9050AC 7.8% 139.7 / 4.8 51Y208* (TAT→TAG) trmBtRNA (guanosine(46)‑N7)‑methyltransferase TrmB
Reads supporting (aligned to +/- strand):  ref base A (26/21);  new base C (2/2);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.69e-01

AACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACGGCTCCCAATCGGTGGCCATGTGGAACACACCACCGGGCTTGAGCTTGCGACGTACCAGCTCGGCGAACTCCAGCTG  >  NC_002947/5824759‑5825054
                                                                                                                                                  |                                                                                                                                                     
aaCCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAg                                                                                                                                                    >  1:209074/1‑150 (MQ=255)
aaCCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAg                                                                                                                                                    <  2:209074/150‑1 (MQ=255)
aaCCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAg                                                                                                                                                    <  1:373813/150‑1 (MQ=255)
aaCCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAg                                                                                                                                                    >  2:373813/1‑150 (MQ=255)
aaCCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACATTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAg                                                                                                                                                    >  2:756207/1‑150 (MQ=255)
    gCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGc                                                                                                                                                >  1:96295/1‑150 (MQ=255)
    gCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGc                                                                                                                                                >  2:139280/1‑150 (MQ=255)
    gCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGc                                                                                                                                                >  2:678110/1‑150 (MQ=255)
             aGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCg                                                                                                                                       <  1:79160/150‑1 (MQ=255)
                             aaaGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGACCCCCCACCAGTGCCCCGCCGGCGCCCGTCTGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGcc                                                                                                                       <  1:101638/150‑1 (MQ=255)
                                 gCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGa                                                                                                                   >  2:698556/1‑150 (MQ=255)
                                   cGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGc                                                                                                                 >  2:491852/1‑150 (MQ=255)
                                      aTCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTgc                                                                                                              <  1:139280/150‑1 (MQ=255)
                                       tCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCa                                                                                                                      <  1:426770/141‑1 (MQ=255)
                                       tCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCa                                                                                                                      >  2:426770/1‑141 (MQ=255)
                                       tCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTgcg                                                                                                             >  2:538814/1‑150 (MQ=255)
                                              cTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCtcatca                                                                                                      >  1:835666/1‑150 (MQ=255)
                                                   tCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGcc                                                                                                                                               <  2:274577/104‑1 (MQ=255)
                                                   tCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGcc                                                                                                                                               >  1:274577/1‑104 (MQ=255)
                                                     cACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAg                                                                                               >  2:304843/1‑150 (MQ=255)
                                                       ccTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCa                                                                                             <  2:199919/150‑1 (MQ=255)
                                                           ctcGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTa                                                                                         >  2:108882/1‑150 (MQ=255)
                                                                 cTTCAAGTCCCACACCCAGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGc                                                                                   >  2:716154/1‑150 (MQ=255)
                                                                  ttCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGCGCTGCGCTCATCACTTCCAGCATGTACTCGGCa                                                                                  >  1:173696/1‑150 (MQ=255)
                                                                   tCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCAt                                                                                 <  2:71012/150‑1 (MQ=255)
                                                                       gTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACgg                                                                             <  1:113369/150‑1 (MQ=255)
                                                                       gTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACgg                                                                             <  1:756207/150‑1 (MQ=255)
                                                                            acacCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACGGCTccc                                                                        <  2:96295/150‑1 (MQ=255)
                                                                                    tGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCCGGACATAGGTGCCGTCTGCCGCATGGTTGCCATAGCCAGTAGCTGCGGTCATCACTTCCAGCATGTACTCGGCATACGGCTCCCAATCGGTg                                                                <  1:226691/150‑1 (MQ=255)
                                                                                         cAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACGGCTCCCAATCGGTGGCCAt                                                           >  1:472715/1‑150 (MQ=255)
                                                                                             cGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACGGCTCCCAATCGGTGGCCATGTgg                                                       <  1:698556/150‑1 (MQ=255)
                                                                                                  aCCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGtt                                                                                                                                <  1:321584/72‑1 (MQ=255)
                                                                                                  aCCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGtt                                                                                                                                >  2:321584/1‑72 (MQ=255)
                                                                                                                  ttGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCa                                                                                             >  1:67309/1‑91 (MQ=255)
                                                                                                                  ttGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCa                                                                                             <  2:67309/91‑1 (MQ=255)
                                                                                                                  ttGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACGGCTCCCAATCGGTGGCCATGTGGAACACACCACCGGGCTTGAGc                                  >  1:377610/1‑150 (MQ=255)
                                                                                                                  ttGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACGGCTCCCAATCGGTGGCCATGTGGAACACACCACCGGGCTTGAGc                                  >  1:469086/1‑150 (MQ=255)
                                                                                                                         tCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACGGCTCCCAATCGGTGGCCATGTGGAACACACCACCGGGCTTGAGCTTGCGAc                           <  2:51482/150‑1 (MQ=255)
                                                                                                                            ggCGCTCTTCGGGGCGCGGCAGATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACGGCTCCCAATCGGTGGCCATGTGGAACACACCACCGGGCTTGAGCTTGCGACGTa                        >  1:210717/1‑150 (MQ=255)
                                                                                                                                ctctTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATGGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACGGCTCCCAATCGGTGGCCATGTGGAACACACCACCGGGCTTGAGCTTGCGACGTACCAg                    <  1:678110/150‑1 (MQ=255)
                                                                                                                                 tctTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACGGCTCCCAATCGGTGGCCATGTGGAACACACCACCGGGCTTGAGCTTGCGACGTACCAGc                   >  2:422725/1‑150 (MQ=255)
                                                                                                                                   ttCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACGGCTCCCAATCGGTGGCCATGTGGAACACACCACCGGGCTTGAGCTTGc                              <  2:287001/137‑1 (MQ=255)
                                                                                                                                   ttCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACGGCTCCCAATCGGTGGCCATGTGGAACACACCACCGGGCTTGAGCTTGc                              >  1:287001/1‑137 (MQ=255)
                                                                                                                                      ggggCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACGGCTCCCAATCGGTGGCCATGTGGAACACACCACCGGGCTTGAGCTTGCGACGTACCAGCTCGGc              <  1:496959/150‑1 (MQ=255)
                                                                                                                                        ggCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACGGCTCCCAATCGGTGGCCATGTGGAACACACCACCGGGCTTGAGCTTGCGACGTAc                       <  1:650458/139‑1 (MQ=255)
                                                                                                                                        ggCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACGGCTCCCAATCGGTGGCCATGTGGAACACACCACCGGGCTTGAGCTTGCGACGTAc                       >  2:650458/1‑139 (MQ=255)
                                                                                                                                         agacgGCACCTATGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACGGCTCCCAATCGGTGGCCATGTGGAACACACCACCGGGCTTGAGCTTGCGACGTACCAGCTCGGCGaa           >  2:194974/4‑150 (MQ=255)
                                                                                                                                         agacgGCACCTATGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACGGCTCCCAATCGGTGGCCATGTGGAACACACCACCGGGCTTGAGCTTGCGACGTACCAGCTCGGCGaa           <  1:194974/147‑1 (MQ=255)
                                                                                                                                            cgGCACCTATGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACGGCTCCCAATCGGTGGCCATGTGGAACACACCACCGGGCTTGa                                    <  1:799154/122‑1 (MQ=255)
                                                                                                                                            cgGCACCTATGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACGGCTCCCAATCGGTGGCCATGTGGAACACACCACCGGGCTTGa                                    >  2:799154/1‑122 (MQ=255)
                                                                                                                                              gCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCCCTGCCGGCATGTACTCGGCATACGGCTCCCAATCGGTGGCCATGTGGAACACACCACCGGGCTTGAGCTTGCGCCGTACCAGCTCGGCGAACTCCa      >  2:502974/1‑150 (MQ=255)
                                                                                                                                                acaTAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACGGCTCCCAATCGGTGGCCATGTGGAACACACCACCGGGCTTGAGCTTGCGACGTACCAGCTCGGCGAACTCCAGc    >  2:92564/1‑150 (MQ=255)
                                                                                                                                                acaTAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACGGCTCCCAATCGGTGGCCATGTGGAACACACCACCGGCCTTGAGCTTGCGACGTACCAGCTCGGCGAACTCCAGc    <  1:690561/150‑1 (MQ=255)
                                                                                                                                                  aTAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACGGCTCCCAATCGGTGGCCATGTGGAACACACCACCGGGCTTGAGCTTGCGACGTACCAGCTCGGCGAACTCCAGCTg  <  1:716154/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
AACCGCCCTTTTCAGGGCGGTTTTACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCGTCTGCCGCACGGTTGCGATAGCCAGGAGCTGCGCTCATCACTTCCAGCATGTACTCGGCATACGGCTCCCAATCGGTGGCCATGTGGAACACACCACCGGGCTTGAGCTTGCGACGTACCAGCTCGGCGAACTCCAGCTG  >  NC_002947/5824759‑5825054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑