| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 495,972 | G→C | 17.4% | A22G (GCC→GGC) | PP_0408 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 495,972 | 0 | G | C | 17.4% | 65.8 / 6.4 | 28 | A22G (GCC→GGC) | PP_0408 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (10/13); new base C (3/2); total (13/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCCTGCAGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTT > NC_002947/495824‑496113 | ggCCTGCAGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGgc < 2:113070/150‑1 (MQ=255) tGCAGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCg > 1:528284/1‑150 (MQ=255) gTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGa > 1:722654/1‑150 (MQ=255) tCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGaa < 1:629120/150‑1 (MQ=255) ctgctgCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAg < 2:528284/150‑1 (MQ=255) tgctgcTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAgg < 2:415092/150‑1 (MQ=255) ctCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGa > 1:370190/1‑150 (MQ=255) ctCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGa < 2:189567/150‑1 (MQ=255) ttGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGCCCTGGCCGCCGat < 1:199241/131‑2 (MQ=255) ttGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGCCCTGGCCGCCGat > 2:199241/1‑130 (MQ=255) gTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGCCCTGGCCGCCGatgc > 1:297350/1‑117 (MQ=255) gTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGCCCTGGCCGCCGatgc < 2:297350/120‑4 (MQ=255) gTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGACGTGCGAt > 2:49102/1‑150 (MQ=255) cATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTATCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATaaa < 2:128488/150‑1 (MQ=255) tcctGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGtgtg < 2:722654/150‑1 (MQ=255) ctGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAgt > 1:1849/1‑145 (MQ=255) ctGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAgt < 2:1849/145‑1 (MQ=255) ctGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGa > 1:821871/1‑150 (MQ=255) ctGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGa < 2:504717/150‑1 (MQ=255) gCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACAt < 2:118218/150‑1 (MQ=255) tGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGa < 2:370190/150‑1 (MQ=255) ttCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCa > 2:231788/1‑150 (MQ=255) cAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAAcc > 1:725244/1‑150 (MQ=255) gCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCa > 2:381965/1‑150 (MQ=255) ccGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAAt < 1:49102/150‑1 (MQ=255) aTGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGCGTGGTAGGACCGAGGCCAGGCAGCACATTGACGGCGTCGTGCGAGAAAGGTTGGACAGGATTCTACGCAAGGACAGAATGAAACCGGCAGGCTAATAGAAtgtg > 2:97160/1‑150 (MQ=255) actCGGCGCCCTGGCCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGcc > 1:829561/3‑150 (MQ=255) gcggcCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCAGCATGGCCGTcac < 2:139896/150‑3 (MQ=255) cggcCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCttt < 1:682618/150‑1 (MQ=255) | GGCCTGCAGGGTCTGCTGCTCAGCCTTGGGCAGCAGCCGTTCCAGCGCCAGTGCATCCTCCTGGCTGCGTTCCAGCAAGGGTGGGCGTGGGGCCTCGGCAGCCGGTGCCATGGCATTTGCAGGTGGTTTTTCGGCATCGGCGGCCAGGGCGCCGAGTGGAAGGACCGAGGCCAGGCAGCACATTGCCAGCGTCGTGCGATAAAGTGTGGACATGAGTCAACCCAAGGCCAGAATGAAACCGGCAGCCTAATAGTATTTGCCCGTGACGGCCATGCTGCATGGCCGTCTTT > NC_002947/495824‑496113 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |