Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,891,328 G→T 12.9% L116M (CTG→ATG)  plpB ← NLPA lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,891,3280GT12.9% 81.8 / 2.6 31L116M (CTG→ATG) plpBNLPA lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (15/12);  new base T (2/2);  total (17/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

TTCTCGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCTTGCCTTCGTTGAAGTTCTGCAGGTACGGCAGCGTCTGGAAGTAGTTGGCGTCCAGACGCTTCTGGTCCACCTGC  >  NC_002947/5891180‑5891476
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ttCTCGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCTTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAgg                                                                                                                                                     <  1:177505/150‑1 (MQ=255)
   tcGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGcc                                                                                                                                                  >  2:52687/1‑150 (MQ=255)
       cGATATCCTTGGGGGTGGCCAGGGCATTCTTCTGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGCGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGa                                                                                                                                              <  1:108413/150‑1 (MQ=255)
             ccTTGGGGGTCGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCATCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACcttct                                                                                                                                        <  1:231879/150‑1 (MQ=255)
               ttGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGTCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCttcttc                                                                                                                                      <  1:569895/150‑1 (MQ=255)
               ttGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCttcttc                                                                                                                                      <  1:785866/150‑1 (MQ=255)
                          cAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCAcc                                                                                                                           >  1:812398/1‑150 (MQ=255)
                                aTTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAggg                                                                                                                     <  1:326577/150‑1 (MQ=255)
                                      cGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTAc                                                                                                               <  2:812398/150‑1 (MQ=255)
                                                                          cagcagcagcGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCtt                                                                           >  2:152425/1‑150 (MQ=255)
                                                                                      tgcgctgctgctgctGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCTTGCCTTCGTTGaa                                                               <  1:189503/144‑1 (MQ=255)
                                                                                                  gctgcCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCAc                                                                                                                            <  1:300554/77‑1 (MQ=255)
                                                                                                  gctgcCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCAc                                                                                                                            >  2:300554/1‑77 (MQ=255)
                                                                                                   ctgcCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCTACCACGGTTTCCAGGTGGGTGCCCTTGCCTTCGTTGAAGTTCTGCAGGTAc                                                  >  2:780968/1‑150 (MQ=255)
                                                                                                        cTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTAGGTGCCCTTGCCTTCGTTGAAGTTCTGCAGGTACGGCAg                                             >  2:1208/1‑150 (MQ=255)
                                                                                                           gTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCATGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAggtgg                                                                                   >  1:701354/1‑109 (MQ=255)
                                                                                                           gTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCATGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAggtgg                                                                                   >  1:701302/1‑109 (MQ=255)
                                                                                                           gTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCATGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAggtgg                                                                                   <  2:701354/109‑1 (MQ=255)
                                                                                                           gTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCATGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAggtgg                                                                                   <  2:701302/109‑1 (MQ=255)
                                                                                                               gggATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTAcaccc                                                                                                     >  1:736899/1‑87 (MQ=255)
                                                                                                               gggATGGCAACGGTTGCGCCTTCTTTCAGCGCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTAcaccc                                                                                                     <  2:736899/87‑1 (MQ=255)
                                                                                                                       aaCGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCTTGCCTTCGTTGAAGTTCTGCAGGTACGGCAGCGTCTGGAAGTAGtt                              >  1:439889/1‑150 (MQ=255)
                                                                                                                       aaCGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCTTGCCTTCGTTGAAGTTCTGCAGGTACGGCAGCGTCTGGAAGTAGtt                              >  2:236001/1‑150 (MQ=255)
                                                                                                                               cgcCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCTTGCCTTCGTTGAAGTTCTCCAGGTACGGCAGCGTCTGGAAGTAGTTGGCGTCCa                      >  1:315863/1‑150 (MQ=255)
                                                                                                                                 ccTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCTTGCCTTCGTTGAAGTTCTGCAGGTACGGCAGCGTCTGGAAGTAGTTGGCGTCCa                      >  2:334193/1‑148 (MQ=255)
                                                                                                                                 ccTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCTTGCCTTCGTTGAAGTTCTGCAGGTACGGCAGCGTCTGGAAGTAGTTGGCGTCCa                      <  1:334193/148‑1 (MQ=255)
                                                                                                                                  cttcttTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCTTGCCTTCGTTGAAGTTCTGCAGGTACGGCAGCGTCTGGAAGGAGTTGGTGTCCAGAc                   >  2:196663/1‑150 (MQ=255)
                                                                                                                                   ttcttTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCTTGCCTTCGTTGAAGTTCTGCAGGTACGGCAGCGTCTGGAAGTAGTTGGCGTCCAGACg                  >  1:698105/1‑150 (MQ=255)
                                                                                                                                         cAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCTTGCCTTCGTTGAAGTTCTGCAGGTACGGCAGCGTCTGGAAGTAGTTGGCGTCCAGACGCTTCTg            <  2:698105/150‑1 (MQ=255)
                                                                                                                                          aGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCTTGCCTTCGTTGAAGTTCTGCAGGTACGGCAGCGTCTGGAAGTAGTTGGCGTCCAGACGCTTCTgg           <  2:112933/150‑1 (MQ=255)
                                                                                                                                                 tcAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCTTGCCTTCGTTGAAGTTCTGCAGGTACGGCAGCGTCTGGAAGTAGTTGGCGTCCAGACGCTTCTGGTCCACCt    >  2:131066/1‑150 (MQ=255)
                                                                                                                                                   aGGGCCTTGACCTTCTTCGAGTAGCCACCGAATGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCTTGCCTTCGTTGAAGTTCTGCAGGTACGGCAGCGTCTGGAAGTAGTTGGCGTCCAGACGCTTCTGGTCCACCTGc  >  1:840816/1‑150 (MQ=255)
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TTCTCGGCGATATCCTTGGGGGTGGCCAGGGCATTCTTCGGGTCTTTCAAGGTAATCAGGCCGGCCTTCTGCAGCAGCAGCAGCGCACGGCCGCTGTTGCTGCCCTCGTTAGGGATGGCAACGGTTGCGCCTTCTTTCAGCTCGCTCAGGGCCTTGACCTTCTTCGAGTAGCCACCGAAGGGCTCTACGTGTACACCCACCACGGTTTCCAGGTGGGTGCCCTTGCCTTCGTTGAAGTTCTGCAGGTACGGCAGCGTCTGGAAGTAGTTGGCGTCCAGACGCTTCTGGTCCACCTGC  >  NC_002947/5891180‑5891476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑