Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,936,992 C→T 14.8% N151N (AAC→AAT)  yhiI → translation‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,936,9920CT14.8% 58.4 / 4.4 27N151N (AAC→AAT) yhiItranslation‑like protein
Reads supporting (aligned to +/- strand):  ref base C (9/14);  new base T (2/2);  total (11/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01

CGCCGCACAGGCCAGCGTGCAACTGCGCCAGAGTGAACTGCTGTTGGCCAGCCAGGAGCTCAAGCGCGTACGCGAAATCTTCCAGCGCAAGTTCGCCAGCCAGCAGTTGCTCGACCAACAGCAGGCGCGCTTTGACACGGCCAACGCAGCAGTAGTCGCTGCCCGCGCCCAACTGGCAGCGATCAAGGCCGCCATTGGCGCCGCCGAAGCCCAAGTGGCGCAGCTCACCAGCGAAATCGACGACAGCAGCCTGCGTGCGCCCATCAACGGCATCATCCAGCTGCGC  >  NC_002947/5936848‑5937133
                                                                                                                                                |                                                                                                                                             
cgccgcACAGGCCAGCGTGCAACTGCGCCAGAGTGAACTGCTGTTGGCCAGCCAGGAGCTCAAGCGCGTACGCGAAATCTTCCAGCGCAAGTTCGCCAGCCAGCAGTTGCTCGACCAACAGCAGGCGCGCTTTGACACGGCCAACgcagc                                                                                                                                          <  1:10334/150‑1 (MQ=255)
 gccgcACAGGCCAGCGTGCAACTGCGCCAGAGTGAACTGCTGTTGGCCAGCCAGGAGCTCAAGCGCGTACGCGAAATCTTCCAGCGCAAGTTCGCCAGCCAGCAGTTGCTCGACCAACAGCAGGCGCGCTTTGACACGGCCAACgcagca                                                                                                                                         >  1:496717/1‑150 (MQ=255)
     cacaGGCCAGCGTGCAACTGCGCCAGAGTGAACTGCTGTTGGCCAGCCAGGAGCTCAAGCGCGTACGCGAAATCTTCCAGCGCAAGTTCGCCAGCCAGCAGTTGCTCGACCAACAGCAGGCGCGCTTTGACACGGCCAACGCAGCagtac                                                                                                                                     <  2:638581/150‑2 (MQ=255)
        aGGCCAGCGTGCAACTGCGCCAGAGTGAACTGCTGTTGGCCAGCCAGGAGCTCAAGCGCGTACGCGAAATCTTCCAGCGCAAGTTCGCCAGCCAGCAGTTGCTCGACCAACAGCAGGCGCGCTTTGACACGGCCAACGCAGCAGTAGTCg                                                                                                                                  >  1:686140/1‑150 (MQ=255)
                               agTGAACTGCTGTTGGCCAGCCAGGAGCTCAAGCGCGTACGCGAAATCTTCCAGCGCAAGTTCGCCAGCCAGCAGTTGCTCGACCAACAGCAGGCGCGCTTTGACACGGCCAACGCAGCAGTAGTCGCTGCCCGCGCCCAACTGGCAGCg                                                                                                           >  2:205159/1‑150 (MQ=255)
                                 tGAACTGCTGTTGGCCAGCCAGGAGCTCAAGCGCGTACGCGAAATCTTCCAGCGCAAGTTCGCCAGCCAGCAGTTGCTCGACCAACAGCAGGCGCGCTTTGACACGGCCAACGCAGCAGTAGTCGCTGCCCGCGCCCAACTGGCAGCGAt                                                                                                         <  2:686140/150‑1 (MQ=255)
                                               ccagccagGAGCTCAAGCGCGTACGCGAAATCTTCCAGCGCAAGTTCGCCAGCCAGCAGTTGCTCGACCAACAGCAGGCGCGCTTTGACACGGCCAACGCAGCAGTAGTCGCTGCCCGCGCCCAACTGGCAGCGATCAAGGCCGCCATTg                                                                                           <  1:106157/150‑1 (MQ=255)
                                                           tCAAGCGCGTACGCGAAATCTTCCAGCGCAAGTTCGCCAGCCAGCAGTTGCTCGAACAACCGCAGGCGCGATTTGACACGGCCAACGCAGCATGAGGCGCTGCCCGCGCCGAACTGGCAGCGATCAAGGCCGCCATTGGCGCCGCCGAAg                                                                               <  2:736770/150‑1 (MQ=255)
                                                                 gcgTACGCGAAATCTTCCAGCGCAAGTTCGCCAGCCAGCAGTTGCTCGACCAACAGCAGGCGCGCTTTGACACGGCCAACGCAGCAGTAGTCGCTGCCCGCGCCCAACTGGCAGCGATCAAGGCCGCCATTGGCGCCGCCGAAGCCCAAg                                                                         <  2:433097/150‑1 (MQ=255)
                                                                               ttCCAGCGCAAGTTCGCCAGCCAGCAGTTGCTCGACCAAGAGCAGGCCCGCTTTGACACGGCCAACGCAGCAATAATCGCGGCCCGCGCCCAACTGGCAGCGATCAAGGCCGCCATCGGCCCCGCCGAAGACCCAGTGGCGCAGCTCAcc                                                           >  2:118193/1‑150 (MQ=255)
                                                                                     cgcAAGTTCGCCAGCCAGCAGTTGCTCGACCAACAGCAGGCGCGCTTTGACACGGCCAACGCAGCAGTAGTCGCTGCCCGCGCCCAACTGGCAGCGATCAAGGCCGCCATTGGCGACGCCGAAGCCCGAGTGGCGCAGCTCACCCGCGaa                                                     <  1:323217/150‑1 (MQ=255)
                                                                                           ttCGCCAGCCAGCAGTTGCTCGACCAACAGCAGGCGCGCTTTGACACGGCCAACGCAGCAGTAGTCGCTGCCCGCGCCCAACTGGCAGCGATCAAGGCCGCCATTGGCGCCGCCGAAGCCCAAGTGGCGCAGCTCACCAGCGAAATcgac                                               <  2:719386/150‑1 (MQ=255)
                                                                                           ttCGCCAGCCAGCAGTTGCTCGACCAACAGCAGGCGCGCTTTGACACGGCCAACGCAGCAGTAGTCGCTGCCCGCGCCCAACTGGCAGCGATCAAGGCCGCCATTGGCGCCGCCGAAGCCCAAGTGGCGCAGCTCACCAGCGAAATcgac                                               <  2:496717/150‑1 (MQ=255)
                                                                                             cGCCAGCCAGCAGTTGCTCGACCAACAGCAGGCGCGCTTTGACACGGCCAACGCAGCAGTAGTCGCTGCCCGCGCCCAACTGGCAGCGATCAAGGCCGCCATTGGCGCCGCCGAAGCCCAAGTGGCGCAGCTCACCAGCGAAATcgacga                                             <  1:594003/150‑1 (MQ=255)
                                                                                                   ccagcAGTTGCTCGACCAACAGCAGGCGCGCTTTGACACGGCCAACGCAGCAGTAGTCGCTGCCCGCGCCCAACTGGCAGCGATCAAGGCCGCCATTGGCGCCGCCGAAGCCCAAGTGGCGCAGCTCACCAGCGAAATCGACGAcagcag                                       >  2:201651/1‑150 (MQ=255)
                                                                                                    cagcagTTGCTCGACCAACAGCAGGCGCGCTTTGACACGGCCAACGCACAATTAATCGCTGCCCGCGCCCAACTGGCAGCGGTCAAGGCCGCCATTGGCGCCGCCGAAGCCCCAGTGGCGCAGCTCACCAGCGAAATCGACGACagcagc                                      <  1:692482/150‑1 (MQ=255)
                                                                                                            gCTCGACCAACAGCAGGCGCGCTTTGACACGGCCAACGCAGCAGTAGTCGCTGCCCGCGCCCAACTGGCAGCGAACAAGGCCGCCATTGGCGCCGCCGAAGCCCAAGTGGCTCAGCTCACCAGCGAAATCGACGACAGCAGCCtgcgtgc                              >  1:696736/1‑150 (MQ=255)
                                                                                                             cTCGACCAACAGCAGGCGCGCTTTGACACGGCCAACGCAGCAGTAGTCGCTGCCCGCGCCCAACTGGCAGCGAt                                                                                                         >  2:325175/1‑74 (MQ=255)
                                                                                                             cTCGACCAACAGCAGGCGCGCTTTGACACGGCCAACGCAGCAGTAGTCGCTGCCCGCGCCCAACTGGCAGCGAt                                                                                                         <  1:325175/74‑1 (MQ=255)
                                                                                                              tCGACCAACAGCAGGCGCGCTTTGACACGGCCAACGCAGCAGTAGTCGCTGCCCGCGCCCAACTGGCAGCGATCAAGGCCGCCATTGGCGCCGCCGAAGCCCAAGTGGCGCAGCTCACCAGCGAAATCGACGACAGCAGCCTGCGTgcgc                            >  2:831290/1‑150 (MQ=255)
                                                                                                                    aaCAGCAGGCGCGCTTTGACACGGCCAATGCAGCAGTAGTCGCTGCCCGCGCCCAACTGGCAGCGATCAAGGCCGCCATTGGCGCCGCCGAAGCCCAAGTGGCGCAGCTCACCAGCGAAATcgacga                                             <  2:605218/127‑1 (MQ=255)
                                                                                                                    aaCAGCAGGCGCGCTTTGACACGGCCAATGCAGCAGTAGTCGCTGCCCGCGCCCAACTGGCAGCGATCAAGGCCGCCATTGGCGCCGCCGAAGCCCAAGTGGCGCAGCTCACCAGCGAAATcgacga                                             <  2:605233/127‑1 (MQ=255)
                                                                                                                    aaCAGCAGGCGCGCTTTGACACGGCCAATGCAGCAGTAGTCGCTGCCCGCGCCCAACTGGCAGCGATCAAGGCCGCCATTGGCGCCGCCGAAGCCCAAGTGGCGCAGCTCACCAGCGAAATcgacga                                             >  1:605218/1‑127 (MQ=255)
                                                                                                                    aaCAGCAGGCGCGCTTTGACACGGCCAATGCAGCAGTAGTCGCTGCCCGCGCCCAACTGGCAGCGATCAAGGCCGCCATTGGCGCCGCCGAAGCCCAAGTGGCGCAGCTCACCAGCGAAATcgacga                                             >  1:605233/1‑127 (MQ=255)
                                                                                                                      cagcagGCGCGCTTTGACACGGCCAACGCAGCAGTAGTCGCTGCCCGCGCCCAACTGGCAGCGATCAAGGCCGCCATTGGCGCCGCCGAAGCCCAAGTGGCGCAGCTCACCAGCGAAATCGACGACAGCAGCCTGCGTGCGCCCATCAAc                    <  1:518608/150‑1 (MQ=255)
                                                                                                                         cagGCGCGCTTTGACACGGCCAACGCAGCAGTAGTCGCTGCCCGCGCCCAACTGGCAGCGATCAAGGCCGCCATTGGCGCCGCCGAAGCCCAAGTGGCGCAGCTCACCAGCGAAATCGACGACAGCAGCCTGCGTGCGCCCATCAACGGc                 <  1:297055/150‑1 (MQ=255)
                                                                                                                                        acGGCCAACGCAGCAGTAGTCGCTGCCCGCGCCCAACTGGCAGCGATCAAGGCCGCCATTGGCGCCGCCGAAGCCCAAGTGGCGCAGCTCACCAGCGAAATCGACGACAGCAGCCTGCGTGCGCCCATCAACGGCATCATCCAGCTgcgc  >  2:274520/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                             
CGCCGCACAGGCCAGCGTGCAACTGCGCCAGAGTGAACTGCTGTTGGCCAGCCAGGAGCTCAAGCGCGTACGCGAAATCTTCCAGCGCAAGTTCGCCAGCCAGCAGTTGCTCGACCAACAGCAGGCGCGCTTTGACACGGCCAACGCAGCAGTAGTCGCTGCCCGCGCCCAACTGGCAGCGATCAAGGCCGCCATTGGCGCCGCCGAAGCCCAAGTGGCGCAGCTCACCAGCGAAATCGACGACAGCAGCCTGCGTGCGCCCATCAACGGCATCATCCAGCTGCGC  >  NC_002947/5936848‑5937133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑