Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,951,040	C→A	14.9%	R658L (CGT→CTT)	ppk ←	polyphosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,951,040	0	C	A	14.9%	103.5 / 8.7	41	R658L (CGT→CTT)	ppk	polyphosphate kinase
Reads supporting (aligned to +/- strand):  ref base C (13/21);  new base A (3/3);  total (17/24)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

CTGGTTGCCGGTCGGGGTGCTGCGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGATGTTGTGCGAAACCCCCG  >  NC_002947/5950895‑5951181                                                                                                                                                  |                                                                                                                                              cTGGTTGCCGGTCGGGGTGCTGCGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCtt                                                                                                                                           <  1:461331/150‑1 (MQ=255)                      gcgcACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCtccatcca                                                                                                                      <  1:515532/150‑1 (MQ=255)                          aCGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGTTCCATCCAGTCg                                                                                                                  >  1:783715/1‑150 (MQ=255)                          aCGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCg                                                                                                                  >  1:502183/1‑150 (MQ=255)                                  cccGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGa                                                                                                          <  2:470416/150‑1 (MQ=255)                                   ccGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGAc                                                                                                         <  1:778511/150‑1 (MQ=255)                                    cGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACa                                                                                                        >  2:274291/1‑150 (MQ=255)                                          gCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACACTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGa                                                                                                  >  2:625072/1‑150 (MQ=255)                                            tGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATc                                                                                                >  2:693854/1‑150 (MQ=255)                                              cAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGCCACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTg                                                                                              <  1:701367/150‑1 (MQ=255)                                                 ggTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGctc                                                                                           <  1:140561/150‑1 (MQ=255)                                                     cAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACACTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTcg                                                                                       <  1:625072/150‑1 (MQ=255)                                                             ggTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCAtt                                                                               <  2:193156/150‑1 (MQ=255)                                                                   gTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCATGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGaa                                                                         <  2:455517/150‑1 (MQ=255)                                                                    tCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGa                                                                             >  1:254274/1‑145 (MQ=255)                                                                    tCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGa                                                                             <  2:254274/145‑1 (MQ=255)                                                                            gTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAacac                                                                <  2:757907/150‑1 (MQ=255)                                                                              aGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCt                                                                                               <  1:462731/117‑1 (MQ=255)                                                                              aGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCt                                                                                               >  2:462731/1‑117 (MQ=255)                                                                                ccTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAg                                                                                                       <  2:472554/107‑1 (MQ=255)                                                                                ccTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAg                                                                                                       >  1:472554/1‑107 (MQ=255)                                                                                 cTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCcgcg                                                           >  1:842721/1‑150 (MQ=255)                                                                                  ttCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACAAGCGTGTCGAgac                                                                                                                                   <  1:853124/77‑3 (MQ=255)                                                                                  ttCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACAAGCGTGTCGAgac                                                                                                                                   >  2:853124/1‑75 (MQ=255)                                                                                           cttcttCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGTTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAg                                                 <  2:783715/150‑1 (MQ=255)                                                                                           cttcttCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACAAGCGTGTCGAgac                                                                                                                                   <  1:35094/68‑3 (MQ=38)                                                                                           cttcttCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACAAGCGTGTCGAgac                                                                                                                                   >  2:35094/1‑66 (MQ=38)                                                                                                    ccGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACATGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGcc                                        >  1:150454/1‑150 (MQ=255)                                                                                                    ccGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATc                                                                                                <  2:345844/94‑1 (MQ=255)                                                                                                    ccGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATc                                                                                                >  1:345844/1‑94 (MQ=255)                                                                                                    ccGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGcc                                        >  2:616800/1‑150 (MQ=255)                                                                                                                          tCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCTATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGAtg                  <  2:268419/150‑1 (MQ=255)                                                                                                                             atcgtgAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGAtgttg               <  2:732406/145‑1 (MQ=255)                                                                                                                               cGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGATGTtgtg             <  1:693854/150‑1 (MQ=255)                                                                                                                               cGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCACTCGGCGCTGGACAGGGAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGATGTtgtg             >  1:550855/1‑150 (MQ=255)                                                                                                                                gggAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGATGTTGTGc            <  2:842721/150‑1 (MQ=255)                                                                                                                                      cAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGAtgttgt              <  2:166973/142‑1 (MQ=255)                                                                                                                                      cAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGAtgttgt              >  1:166973/1‑142 (MQ=255)                                                                                                                                        aacctcGACAAGCGTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGc                                            >  1:826083/4‑110 (MQ=255)                                                                                                                                        aacctcGACAAGCGTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGc                                            <  2:826083/107‑1 (MQ=255)                                                                                                                                          tctcGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGATGTTGTGCGAAACCCCCg  <  2:583644/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                              CTGGTTGCCGGTCGGGGTGCTGCGCACGTAGCGCCCGTCTGGCTGCAAGGTCCAGGCGTGGGTATTGTCGGTCAGGTAGCCTTCCAGCTCCTTCTTCACCCGCAGCAACAGTTTCTTGCCTTCCACCGGGAAGCAGGTCTCGACACGCTTGTCGAGGTTGCGCTCCATCCAGTCGGCGCTGGACAGGTAGATCTGCTCTTCGCCGCCATTGAGGAAGTAGAACACCCGCGTGTGCTCGAGGAAGCGGCCAATGATCGAGCGCACCTGGATGTTGTGCGAAACCCCCG  >  NC_002947/5950895‑5951181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑